1887

Abstract

The Tyzzer’s disease organism was grown in primary monolayer cultures of adult mouse hepatocytes prepared by collagenase perfusion. The organisms produced a plaque-like cytopathic effect involving almost the whole culture around 72 h post-infection when the bacterial growth reached a maximum. The organisms showed specific immunofluorescence, and electron microscopy revealed that intracellular organisms had peritrichous flagella and underwent cell division. After intravenous inoculation of the infected cell culture into mice, necrotic hepatitis was produced and the organisms, recovered from the liver lesion, could be propagated in primary culture of mouse hepatocytes.

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-129-2-277
1983-02-01
2024-12-14
Loading full text...

Full text loading...

/deliver/fulltext/micro/129/2/mic-129-2-277.html?itemId=/content/journal/micro/10.1099/00221287-129-2-277&mimeType=html&fmt=ahah

References

  1. Arnheiter H. 1980; Primary monolayer culture of adult mouse hepatocytes - a model for the study of hepatotropic viruses. Archives of Virology 63:11–22
    [Google Scholar]
  2. Craigie J. 1966; ‘Bacillus piliformis’ (Tyzzer) and Tyzzer's disease of the laboratory mouse. I. Propagation of the organism in embryonated eggs. Proceedings of the Royal Society B165:35–60
    [Google Scholar]
  3. Fujiwara K. 1967; Complement fixation reaction and agar gel double diffusion test in Tyzzer’s disease of mice. Japanese Journal of Microbiology 11:103–117
    [Google Scholar]
  4. Fujiwara K. 1971; Problems in checking inapparent infections in laboratory mouse colonies: an attempt at serological checking by anamnestic response. In Defining of Laboratory Animals pp. 77–92 Schneider H. A. Edited by Washington, D.C.: National Academy of Sciences;
    [Google Scholar]
  5. Fujiwara K. 1978; Tyzzer’s disease. Japanese Journal of Experimental Medicine 48:467–480
    [Google Scholar]
  6. Fujiwara K., Takagaki Y., Maejima K., Kato K., Naiki M., Tajima Y. 1963; Tyzzer’s disease in mice: pathologic studies on experimentally infected animals. Japanese Journal of Experimental Medicine 33:183–202
    [Google Scholar]
  7. Fujiwara K., Takagaki Y., Naiki M., Maejima K., Tajima Y. 1964; Tyzzer’s disease in mice. Effects of corticosteroids on the formation of liver lesions and the level of blood transaminases in experimentally infected animals. Japanese Journal of Experimental Medicine 34:59–75
    [Google Scholar]
  8. Fujiwara K., Takahashi R., Kurashina H., Matsunuma N. 1969; Protective serum antibodies in Tyzzer’s disease of mice. Japanese Journal of Experimental Medicine 39:491–504
    [Google Scholar]
  9. Fujiwara K., Yamada A., Ogawa H., Oshima Y. 1971; Comparative studies on the Tyzzer’s organisms from rats and mice. Japanese Journal of Experimental Medicine 41:125–133
    [Google Scholar]
  10. Fujiwara K., Kurashina H., Magaribuchi T., Takenaka S., Yokoiyama S. 1973; Further observations on the difference between Tyzzer's organisms from mice and those from rats. Japanese Journal of Experimental Medicine 43:307–315
    [Google Scholar]
  11. Ganaway J. R., Allen A. M., Moore T. D. 1971a; Tyzzer’s disease of rabbits: isolation and propagation of Bacillus piliformis (Tyzzer) in embryonated eggs. Infection and Immunity 3:429–437
    [Google Scholar]
  12. Ganaway J. R., Allen A. M., Moore T. D. 1971b; Tyzzer’s disease. American Journal of Pathology 64:717–732
    [Google Scholar]
  13. Kanazawa K., Imai A. 1959; Pure culture of the pathogenic agent of Tyzzer’s disease of mice. Nature; London: 1841810–1811
    [Google Scholar]
  14. Rights F. L., Jackson E. B., Smadel J. E. 1947; Observations on Tyzzer’s disease in mice. American Journal of Pathology 23:627–635
    [Google Scholar]
  15. Seglen P. O. 1976; Preparation of isolated rat liver cells. Methods in Cell Biology 13:29–83
    [Google Scholar]
  16. Tanaka K., Sato M., Tomita Y., Ichihara A. 1978; Biochemical studies on liver functions in primary cultured hepatocytes of adult rats. I. Hormonal effects on cell viability and protein synthesis. Journal of Biochemistry 84:937–946
    [Google Scholar]
  17. Tyzzer E. E. 1917; A fatal disease of the Japanese waltzing mouse caused by a spore-bearing bacillus {Bacillus piliformis N. sp.). Journal of Medical Research 37:307–338
    [Google Scholar]
/content/journal/micro/10.1099/00221287-129-2-277
Loading
/content/journal/micro/10.1099/00221287-129-2-277
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error