1887

Abstract

Application of a viscometric assay to the haemagglutination induced by strain 438 showed that shear forces can enhance the strength of bacterial adhesion. The D-mannose/L-fucose-sensitive reaction proceeded in two phases, an initial phase in which the degree of aggregation remained constant during shearing and a second stage, induced by shear, in which agglutination was enhanced as shear was maintained. The results strongly paralleled those found in studies of concanavalin A-induced haemagglutination, providing good evidence that adhesion in this species took place via lectin-like molecules. Methyl---mannoside, which strongly inhibits haemagglutination in this system, would not fully reverse the shear-dependent reaction. EGTA inhibited and reversed both phases, however. The effects of bacterial concentration, temperature, time of growth, pH, and a spectrum of monosaccharide inhibitors were also studied. The results demonstrated that the shear-dependent reaction has a number of features which distinguish it from the initial stage of haemagglutination, implying differences in the underlying biochemical mechanisms involved.

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-129-12-3661
1983-12-01
2022-01-25
Loading full text...

Full text loading...

/deliver/fulltext/micro/129/12/mic-129-12-3661.html?itemId=/content/journal/micro/10.1099/00221287-129-12-3661&mimeType=html&fmt=ahah

References

  1. Brooks D. E., Trust T. J. 1980; An improved technique for the study of bacterial adhesion to surfaces. In Microbriai Adhesion to Surfaces pp. 513–515 Edited by Berkeley R. C. W., Lynch J. M., Moiling V., Butter P. R., Vincent B. Chichester: Ellis Horwood;
    [Google Scholar]
  2. Brooks D. E., Goodwin J. W., Seaman G.V.F. 1974; Rheology of erythrocyte suspensions: electrostatic factors in the dextran-mediated aggregation of erythrocytes. Biorheology 11:69–77
    [Google Scholar]
  3. Brooks D. E., Greig R. G., Janzen J. 1980; Mechanisms of erythrocyte aggregation. In Erythrocyte Mechanics and Blood Flow pp. 119–140 Edited by Cokelet G., Meiselman H., Brooks D. E. New York: Alan R. Liss;
    [Google Scholar]
  4. Bryers J. D., Characklis W. G. 1982; Processes governing primary biofilm formation. Biotechnology and Bioengineering 24:2451–2476
    [Google Scholar]
  5. Duguid J. P., Old D. C. 1980; Adhesive properties of Enterobacteriaceae. In Receptors and Recognition Series B 6 pp. 185–217 Edited by Beachey E. H. London: Chapman & Hall;
    [Google Scholar]
  6. Fowler H. W., Mckay A. J. 1980; The measurement of microbial adhesion. In Microbial Adhesion to Surfaces pp. 143–161 Edited by Berkeley R. C. W., Lynch J. M., Moiling V., Rutter P. R., Vincent B. Chichester: Ellis Horwood;
    [Google Scholar]
  7. Greig R. G., Brooks D. E. 1979; Shear-induced concanavalin A agglutination of human erythrocytes. Nature, London 282:738–739
    [Google Scholar]
  8. Greig R. G., Brooks D. E. 1981; Enhanced concanavalin A agglutination of trypsinised erythrocytes is due to a specific class of aggregation. Biochemica et biophysica acta 641:410–415
    [Google Scholar]
  9. Heard D. H., Seaman G.V.F. 1960; The influences of pH and ionic strength on the electrokinetic ability of the human erythrocyte membrane. Journal of General Physiology 43:635–654
    [Google Scholar]
  10. Jones G. W. 1977; The attachment of bacteria to the surfaces of animal cells. In Receptors and Recognition, Series B 3 pp. 139–176 Edited by Reissig J. L. London: Chapman & Hall;
    [Google Scholar]
  11. Keusch G. T. 1979; Specific membrane receptors: pathogenic and therapeutic implications in infectious disease. Reviews on Infectious Diseases 1:517–529
    [Google Scholar]
  12. Trust T. J., Courtice L. D., Atkinson H. M. 1980; Hemagglutination properties of Aeromonas. In Fish Diseases pp. 218–223 Edited by Ahne W. Berlin: Springer-Verlag;
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-129-12-3661
Loading
/content/journal/micro/10.1099/00221287-129-12-3661
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error