1887

Abstract

Two plasmids transferred from enterotoxigenic (ETEC) of serotype 06. H16 and biotypes A and C coded for mannose-resistant haemagglutination (MRHA) and production of heat-stable enterotoxin (ST) and heat-labile enterotoxin (LT). Both plasmids were non-autotransferring being mobilized most efficiently by the R plasmid R100-1. They were similar in their genetic properties being incompatible with each other and plasmids of the Inc group FI. The wild-type strains produced the colonization factor antigen II (CFA/II) which was made up of different coli surface antigens (CS). The biotype A strains produced CS1 and CS3 while the biotype C strains produced CS2 and CS3. These three antigens have the ability to cause MRHA. When plasmids coding for MRHA were transferred to K12 strains, the degree of haemagglutination was markedly reduced and only CS3 was produced. When both plasmids were transferred back into biotype A strains, good MRHA was restored and the strains produced CS1 and CS3. In a biotype C strain CS2 and CS3 were formed. The production of the antigens was compared by enzyme-linked immunosorbent assay (ELISA). The strains were also examined by electron microscopy where it was found that CS1 and CS2 were fimbrial antigens while CS3 was not.

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1983-12-01
2024-04-25
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References

  1. Anderson E. S., Threlfall E. J. 1974; The characterisation of plasmids in the enterobacteria. Journal of Hygiene 72:471–487
    [Google Scholar]
  2. Awad-Masalmeh A., Moon H. W., Runnels P. L., Schneider R. A. 1982; Pilus production, hemagglutination and adhesion by porcine strains of enterotoxigenic Escherichia coli lacking K88, K99, and 987P antigens. Infection and Immunity 35:305–313
    [Google Scholar]
  3. Birnboim H. C., Doly J. 1979; A rapid alkaline extraction procedure for screening recombinant plasmid DNA. Nucleic Acids Research 7:1513–1523
    [Google Scholar]
  4. Cravioto A., Scotland S. M., Rowe B. 1982; Hemagglutination activity and colonization factor antigens I and II in enterotoxigenic and nonenterotoxigenic strains of Escherichia coli isolated from humans. Infection and Immunity 36:189–197
    [Google Scholar]
  5. Dean A. G., Ching Y.-C., Williams R. G., Harden L. B. 1972; Test tor Escherichia coh enterotoxin using infant mice. Application in a study of diarrhoea in children in Honolulu. Journal of Infectious Diseases 125:407–411
    [Google Scholar]
  6. Donta S. T., Moon H. W., Whipp S. C. 1974; Detection of heat-labile Escherichia coli enterotoxin with the use of adrenal cells in tissue culture. Science 183:334–336
    [Google Scholar]
  7. Duguid J. P., Smith I. W., Dempster G., Edmunds P. N. 1955; Non-flagellar filamentous appendages (fimbriae) and haemagglutinating activities in Bacterium coli. Journal of Pathology and Bacteriology 70:335–348
    [Google Scholar]
  8. Duguid J. P., Clegg S., Wilson M. I. 1979; The fimbrial and non-fimbrial haemagglutinins of Escherichia coli. Journal of Medical Microbiology 12:213–227
    [Google Scholar]
  9. Eshdat Y., Ofek I., Yashouv-Gan, Sharon N., Mirelman D. 1978; Isolation of a mannose- specific lectin from Escherichia coli and its role in the adherence of the bacteria to epithelial cells. Biochemical and Biophysical Research Communications 85:1551–1559
    [Google Scholar]
  10. Evans D. G., Evans D. J. 1978; New surface- associated heat-labile colonization factor antigen (CFA/II) produced by enterotoxigenic Escherichia coli of serogroups 06 and 08. Infection and Immunity 21:638–647
    [Google Scholar]
  11. Evans D. G., Silver R. P., Evans D. J., Chase D. G., Gorbach S. L. 1975; Plasmid-controlled colonization factor associated with virulence in Escherichia coli enterotoxigenic for humans. Infection and Immunity 12:656–667
    [Google Scholar]
  12. Faris A., Sellei J., Lindahl M., Wadstrom T. 1982; Haemagglutination of bovine erythrocytes by enterotoxigenic E. coli (ETEC) of 06 serogroup: evidence for glycoconjugate receptor heterogeneity. Zentralblatt für Bakteriologie, Mikrobiologie und Hygiene 253:175–182
    [Google Scholar]
  13. Gaastra W., De Graaf F. K. 1982; Host-specific fimbrial adhesins of non-invasive enterotoxigenic Escherichia coli strains. Microbiological Reviews 46:129–161
    [Google Scholar]
  14. Guerrant R. L., Brunton L. L., Schnaitman T. C., Rebhun L. I., Gilman A. G. 1974; Cyclic adenosine monophosphate and alteration of Chinese hamster ovary cell morphology: a rapid sensitive in vitro assay for the enterotoxins of Vibrio cholerae and Escherichia coli. Infection and Immunity 10:320–327
    [Google Scholar]
  15. Ip S. M., Crichton P. B., Old D. C., Duguid J. P. 1981; Mannose-resistant and eluting haemagglutinins and fimbriae in Escherichia coli. Journal of Medical Microbiology 14:223–226
    [Google Scholar]
  16. Jacob A. E., Shapiro J. A., Yamamoto L., Smith D. I., Cohen S. N., Berg D. 1977; Plasmids studied in Escherichia coli and other enteric bacteria. In DNA Insertion Elements, Plasmids and Episomes pp 607–704 Edited by Bukhari A. I., Shapiro J. A., Adhya S. L. Cold Spring Harbor, New York: Cold Spring Harbor Laboratory;
    [Google Scholar]
  17. Mcconnell M. M., Willshaw G. A., Smith H. R., Scotland S. M., Rowe B. 1979; Transposition of ampicillin resistance to an enterotoxin plasmid in an Escherichia coli strain of human origin. Journal of Bacteriology 139:346–355
    [Google Scholar]
  18. Mcconnell M. M., Smith H. R., Willshaw G. A., Scotland S. M., Rowe B. 1980; Plasmids coding for heat-labile enterotoxin production isolated from Escherichia coli 078: comparison * of properties. Journal of Bacteriology 143:158–167
    [Google Scholar]
  19. Mcconnell M. M., Smith H. R., Willshaw G. A., Field A. M., Rowe B. 1981; Plasmids coding for colonization factor antigen I and heat-stable enterotoxin production isolated from enterotoxigenic Escherichia coli: comparison of their properties. Infection and Immunity 32:927–936
    [Google Scholar]
  20. Ørskov I., Ørskov F. 1977; Special 0:K:H serotypes among enterotoxigenic E. coli strains from diarrhea in adults and children. Medical Microbiology and Immunology 163:99–110
    [Google Scholar]
  21. Penaranda M. E., Mann M. B., Evans D. G., Evans D. J. 1980; Transfer of an ST :LT: CFA/II plasmid into Escherichia coli K-12 strain RR1 by cotransformation with pSC301 plasmid DNA. FEMS Microbiology Letters 8:251–254
    [Google Scholar]
  22. Robinson M. K., Bennett P. M., Falkow S., Dodd H. M. 1980; Isolation of a temperature- sensitive derivative of RP1. Plasmid 3:343–347
    [Google Scholar]
  23. Scotland S. M., Gross R. J., Rowe B. 1977; Serotype-related enterotoxigenicity in Escherichia coli 06.H16 and 0148. H28. Journal of Hygiene 79:395–403
    [Google Scholar]
  24. Smith A. M., Tedder R. S. 1981; Development of an enzyme-linked immunosorbent assay (ELISA) for hepatitis Be antigen and antibody. Journal of Virological Methods 3:1–11
    [Google Scholar]
  25. Smith H. R., Willshaw G. A., Rowe B. 1982; Mapping of a plasmid, coding for colonization factor antigen I and heat-stable enterotoxin production, isolated from an enterotoxigenic strain of Escherichia coli. Journal of Bacteriology 149:264–275
    [Google Scholar]
  26. Smith H. R., Scotland S. M., Rowe B. 1983; Plasmids coding for production of colonization factor antigen II and enterotoxin production in strains of Escherichia coli. Infection and Immunity 40:1236–1239
    [Google Scholar]
  27. Smyth C. J. 1982; Two mannose-resistant haemag- glutinins of enterotoxigenic Escherichia coli of serotype 06:K15:H16 or H isolated from travellers’ and infantile diarrhoea. Journal of General Microbiology 128:2081–2096
    [Google Scholar]
  28. Thomas L. V., Rowe B. 1982; The occurrence of colonization factors (CFA/I, CFA/II and E8775) in enterotoxigenic Escherichia coli from various countries in South East Asia. Medical Microbiology and Immunology 171:85–90
    [Google Scholar]
  29. Thomas L. V., Cravioto A., Scotland S. M., Rowe B. 1982; A new fimbrial antigenic type (E8775) which may represent a colonization factor in enterotoxigenic Escherichia coli in humans. Infection and Immunity 35:1119–1124
    [Google Scholar]
  30. Wadstrom T., Faris A., Freer J., Habte D., Hallberg D., Ljungh A. 1980; Hydrophobic surface properties of enterotoxigenic E. coli (ETEC) with different colonization factors (CFA/I, CFA/II, K88 and K99) and attachment to intestinal epithelial cells. Scandinavian Journal of Infectious Diseases supplement 24:148–153
    [Google Scholar]
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