1887

Abstract

A mutant of , isolated for inability to form asexual spores (conidia) on complete medium, was found to regain the ability to conidiate if the medium was supplemented with arginine On minimal medium the mutant required arginine for growth but at a much lower concentration than that required for conidiation. This mutant, designated , thus defines a phase-critical gene, i.e. a gene whose function is in greater demand for development than for growth. In addition to its aconidial phenotype, the mutant also exhibited (depending on the medium) aberrant sexual development and a low efficiency of conidial germination. In crosses, each of these developmental phenotypes segregated with arginine auxotrophy. Genetic and biochemical analyses showed the mutation to be an allele of the previously described locus, mutants of which lack ornithine transcarbamylase. Arginine-requiring mutants at at least two other loci were also found to be defective in asexual sporulation, but the germination defect appears to be specific to mutants.

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1983-11-01
2022-01-19
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References

  1. Armitt S., Mccullough W., Roberts C. F. 1976; Analysis of acetate non-utilizing (acu) mutants in Aspergillus nidulans. Journal of General Microbiology 92:263–282
    [Google Scholar]
  2. Atfield G. N., Morris C.J.O.R. 1961; Analytical separations by high voltage paper electrophoresis. Amino acids in protein hydrolysates. Biochemical Journal 81:606–614
    [Google Scholar]
  3. Axelrod D. E., Gealt M., Pastushok M. 1973; Gene control of developmental competence inAspergillus nidulans . Developmental Biology 34:9–15
    [Google Scholar]
  4. Bartnik E., Weglenski P. 1974; Regulation of arginine catabolism inAspergillus nidulans . Nature, London 250:590–592
    [Google Scholar]
  5. Brody S. 1981; Genetic and biochemical studies onNeurospora conidia germination and formation. In The Fungal Spore: Morphogenetic Controls pp. 605–626 Edited by Turian G., Hohl H. R. London: Academic Press;
    [Google Scholar]
  6. Clutterbuck A. J. 1969; A mutational analysis of conidial development inAspergillus nidulans . Genetics 63:317–327
    [Google Scholar]
  7. Clutterbuck A. J. 1972; Absence of laccase from yellow-spored mutants ofAspergillus nidulans . Journal of General Microbiology 70:423–435
    [Google Scholar]
  8. Clutterbuck A. J. 1974; Aspergillus nidulans. In Handbook of Genetics 1447–510 Edited by King R. C. New York: Plenum;
    [Google Scholar]
  9. Cove D. J. 1966; The induction and repression of nitrate reductase in the fungusAspergillus nidulans . Biochimica et biophysica acta 113:51–56
    [Google Scholar]
  10. Cramer C. L., Vaughn L. E., Davis R. H. 1980; Basic amino acids and inorganic polyphosphates inNeurospora crassa: independent regulation of vacuolar pools. Journal of Bacteriology 142:945–952
    [Google Scholar]
  11. Cybis J., Piotrowska M., Weglenski P. 1972; The genetic control of the arginine pathways inAspergillus nidulans mutants blocked in arginine biosynthesis. Acta microbiologica polonica Ser. A 4(21):163–169
    [Google Scholar]
  12. Dorn G. 1970; Genetic and morphological properties of undifferentiated and invasive variants ofAspergillus nidulans . Genetics 66:267–279
    [Google Scholar]
  13. Hermann T. E., Kurtz M. B., Champe S. P. 1983; A laccase localized in hiille cells and cleistothecial primordia ofAspergillus nidulans . Journal of Bacteriology 154:955–964
    [Google Scholar]
  14. Kurtz M. B., Champe S. P. 1981; Dominant spore color mutants ofAspergillus nidulans defective in germination and sexual development. Journal of Bacteriology 148:629–638
    [Google Scholar]
  15. Maitra P. K. 1971; Glucose and fructose metabolism in a phosphoglucoisomeraseless mutant ofSaccharomyces cerevisiae . Journal of Bacteriology 107:759–769
    [Google Scholar]
  16. Martinelli S. D., Clutterbuck A. J. 1971; Aquantitative survey of conidiation mutants in Aspergillus nidulans . Journal of General Microbiology 69:261–268
    [Google Scholar]
  17. Nurse P., Wiemken A. 1974; Amino acid pools and metabolism during the cell division cycle of arginine-grownCandida utilis . Journal of Bacteriology 117:1108–1116
    [Google Scholar]
  18. Pontecorvo G., Roper J. A., Hemmons L. M., Macdonald K. D., Bufton A.W.J. 1953; The genetics ofAspergillus nidulans . Advances in Genetics 5:141–238
    [Google Scholar]
  19. Schmit J. C., Brody S. 1976; Biochemical genetics ofNeurospora crassa conidial germination. Bacteriological Reviews 40:1–41
    [Google Scholar]
  20. Strigini P., Morpurgo G. 1961; Biotin requirement and carbon and sulphur sources inAspergillusandNeurospora . Nature, London 190:557
    [Google Scholar]
  21. Weiss R. L. 1973; Intracellular localization of ornithine and arginine pools inNeurospora . Journal of Biological Chemistry 218:5409–5413
    [Google Scholar]
  22. Wiemken A., Durr M. 1974; Characterization of amino acid pools in the vacuolar compartment ofSaccharomyces cerevisiae . Archives of Microbiology 101:45–57
    [Google Scholar]
  23. Wiemken A., Nurse P. 1973; Isolation and characterization of the amino-acid pools located within the cytoplasm and vacuoles ofCandida utilis . Planta 109:293–306
    [Google Scholar]
  24. Yager L. N., Kurtz M. B., Champe S. P. 1982; Temperature-shift analysis of conidial development inAspergillus nidulans . Developmental Biology 93:92–103
    [Google Scholar]
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