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Summary: The nuclear (n) and mitochondrial (mt) genomes of the take-all fungus, Gaeumannomyces graminis var. tritici, were examined by reassociation kinetics with Escherichia coli DNA as internal standard. Only one kinetic component was detected in each DNA, with second-order rate constants of 0.022 M−1 s−1 for nDNA and 10.9 M−1 s−1 for mtDNA, corresponding to sequence complexities of 29 × 106 base pairs and 60 × 103 base pairs respectively.