@article{mbs:/content/journal/micro/10.1099/00221287-129-11-3487, author = "Leak, David J. and Dalton, Howard", title = "In vivo Studies of Primary Alcohols, Aldehydes and Carboxylic Acids as Electron Donors for the Methane Mono-oxygenase in a Variety of Methanotrophs", journal= "Microbiology", year = "1983", volume = "129", number = "11", pages = "3487-3497", doi = "https://doi.org/10.1099/00221287-129-11-3487", url = "https://www.microbiologyresearch.org/content/journal/micro/10.1099/00221287-129-11-3487", publisher = "Microbiology Society", issn = "1465-2080", type = "Journal Article", abstract = "C2 to C4 primary alcohols and their corresponding aldehydes were oxidized by type I, type II and type X obligate methanotrophs. Reducing equivalents from each oxidation step could be utilized, in vivo, to stimulate methane mono-oxygenase activity. As neither oxidation step produces NADH directly, these observations are presented as evidence for reverse electron transport in methanotrophs. In type II methanotrophs, 5 mm-acetate, propionate and butyrate also stimulate methane mono-oxygenase activity apparently by inducing the breakdown of poly-β-hydroxybutyrate. subsequent metabolism of β-hydroxybutyrate giving rise to NADH.", }