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C2 to C4 primary alcohols and their corresponding aldehydes were oxidized by type I, type II and type X obligate methanotrophs. Reducing equivalents from each oxidation step could be utilized, in vivo, to stimulate methane mono-oxygenase activity. As neither oxidation step produces NADH directly, these observations are presented as evidence for reverse electron transport in methanotrophs. In type II methanotrophs, 5 mm-acetate, propionate and butyrate also stimulate methane mono-oxygenase activity apparently by inducing the breakdown of poly-β-hydroxybutyrate. subsequent metabolism of β-hydroxybutyrate giving rise to NADH.
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