1887

Abstract

A number of representative species of obligate methane-oxidizing bacteria were surveyed for their ability to fix N by growth experiments and the acetylene reduction test. Although all strains exhibited growth on nitrogen-free plates, only type II organisms and the type X methanotroph (Bath) grew well in nitrogen-free liquid medium and were capable of active acetylene reduction. N-fixation in type II methanotrophs was less senstive to O than in the type X methanotroph (Bath) and batch cultures of type II organisms could be established at pO values of up to 0·2 bar. N-fixation in (Bath) was inhibited at pO values above 0·15 bar and the ‘switch-off’ of nitrogenase activity by ammonia was also observed in this organism.

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1983-11-01
2024-12-02
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References

  1. De Bont J.A.M. 1976; Nitrogen fixation by methane-utilizing bacteria. Antonie van Leeuwenhoek 42:245–253
    [Google Scholar]
  2. De Bont J.A.M., Mulder E.G. 1974; Nitrogen fixation and co-oxidation of ethylene by a methaneutilizing bacterium. Journal of General Microbiology 83:113–121
    [Google Scholar]
  3. Cannon F.C., Riedel G.E. 1979; Overlapping sequences of Klebsiella pneumoniae nif DNA cloned and characterized. Molecular and General Genetics 174:59–66
    [Google Scholar]
  4. Daesch G., Mortenson L.E. 1972; Effect of ammonia on the synthesis and function of the N2- fixing enzyme system in Clostridium pasteurianum. . Journal of Bacteriology 110:103–109
    [Google Scholar]
  5. Dalton H. 1980; Chemoautotrophic nitrogen fixation. In Proceedings of the Phytochemical Society of Europe Symposium, 18. Nitrogen Fixation pp. 177–196 Stewart W.D.P., Gallon J.R. Edited by London: Academic Press.;
    [Google Scholar]
  6. Dalton H., Postgate J.R. 1969; Effect of oxygen on growth of Azotobacter chroococcum in batch and continuous cultures. Journal of General Microbiology 54:463–473
    [Google Scholar]
  7. Dalton H., Whittenbury R. 1976; The acetylene reduction technique as an assay for the nitrogenase activity in the methane oxidizing bacterium Methylococcus capsulatus strain Bath. Archives of Microbiology 109:147–151
    [Google Scholar]
  8. Davis J.B., Coty V.F., Stanley J.P. 1964; Atmospheric nitrogen fixation by methane-oxidizing bacteria. Journal of Bacteriology 88:468–474
    [Google Scholar]
  9. Dilworth M.J. 1966; Acetylene reduction by nitrogen-fixing preparations from Clostridium pasteurianum . Biochimica et biophysica acta 127:285–289
    [Google Scholar]
  10. Drozd J., Postgate J.R. 1970; Effects of oxygen on acetylene reduction, cytochrome content and respiratory activity of Azotobacter chroococcum. . Journal of General Microbiology 63:63–73
    [Google Scholar]
  11. Eady R. 1981; Regulation of nitrogenase activity. In Current Perspectives in Nitrogen Fixation pp. 172–181 Gibson A.H., Newton W.E. Edited by Amsterdam: Elsevier/North-Holland Biomedical Press;
    [Google Scholar]
  12. Falk G., Johansson B.C., Nordlund S. 1982; The role of glutamine synthetase in the regulation of nitrogenase activity (‘switch-off’ effect) in Rhodospirillum rubrum . Archives of Microbiology 132:251–253
    [Google Scholar]
  13. Gordon J.K., Shah V.K., Brill W.J. 1981; Feedback inhibition of nitrogenase. Journal of Bacteriology 148:884–888
    [Google Scholar]
  14. Haaker H., Laane C., Hellingwerf K., Hommer B., Konings W.N., Veeger C. 1982; Shortterm regulation of the nitrogenase activity in Rhodopseudomonas sphaeroides . European Journal of Biochemistry 127:639–645
    [Google Scholar]
  15. Herbert D., Phipps P.J., Strange R.E. 1971; Chemical analysis of microbial cells. Methods in Microbiology 5b:209–344
    [Google Scholar]
  16. Hill S., Postgate J.R. 1969; Failure of putative nitrogen-fixing bacteria to fix nitrogen. Journal of General Microbiology 58:277–285
    [Google Scholar]
  17. Ludden P.W., Okon Y., Burris H. 1978; The nitrogenase system of Spirillum lipoferum . Biochemical Journal 173:1001–1003
    [Google Scholar]
  18. Murrell J.C., Dalton H. 1983; Ammonia assimilation in Methylococcus capsulatus (Bath) and other obligate methanotrophs. Journal of General Microbiology 129:1197–1206
    [Google Scholar]
  19. Murrell J.C., Lidstrom M.E., Dalton H. 1983; Nitrogen fixation in obligate methanotrophs. In Abstracts of the Annual Meeting, American Society for Microbiology, New Orleans 1983 p. 180 Washington, D.C.: American Society for Microbiology;
    [Google Scholar]
  20. Schöllhorn R., Burris R.H. 1967; The reduction of azide by the N2-fixing system. Proceedings of the National Academy of Sciences of the United States of America 57:1317–1323
    [Google Scholar]
  21. Van Dijken J.P., Harder W. 1975; Growth yields of microorganisms on methanol and methane. A theoretical study. Biotechnology and Bioengineering 17:15–30
    [Google Scholar]
  22. Whittenbury R., Dalton H. 1980; The methylo- trophic bacteria. In The Prokaryotes pp. 894–902 Starr M.P., Stolp H., Triiper H.G., Balows A., Schlegel H.G. Edited by New York: SpringerVerlag;
    [Google Scholar]
  23. Whittenbury R., Dalton H. 1982; The obligate methane-oxidizing bacteria and their biotechnological potential. In Basic Biology of New Developments in Biotechnology pp. 439–460 Hoilaender A., Laskin A.I., Rogers P. Edited by New York: Plenum Press;
    [Google Scholar]
  24. Whittenbury R., Phillips K.C., Wilkinson J.F. 1970; Enrichment, isolation and some properties of methane-utilizing bacteria. Journal of General Microbiology, 61:205–218
    [Google Scholar]
  25. Zumft W.G., Alef K., Mummler S. 1981; Regulation of nitrogenase activity in Rhodospirilla- ceae . In Current Perspectives in Nitrogen Fixation pp. 190–193 Gibson A.H., Newton W.E. Edited by Amsterdam: Elsevier/North-Holland Biomedical Press;
    [Google Scholar]
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