Summary: and accumulated zinc from zinc-containing medium. Uptake was biphasic and consisted of an initial, rapid, metabolism-independent binding of zinc to cell surfaces which was followed by slower, metabolism-dependent intracellular uptake of could bind approximately eight times more zinc, per unit surface area, than could Metabolism-dependent zinc uptake followed Michaelis-Menten kinetics with values of 0.09 and 5.00 mM-Zn for and respectively; corresponding values were 0.51 and 9.09 nmol Zn (mg dry wt) min. Zinc uptake by viable cells was not accompanied by potassium release in either yeast, but zinc levels which affected viability in S. cerevisiae caused this yeast to release K. No efflux of K was observed for despite its greater sensitivity to zinc.


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