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Summary: Sporobolomyces roseus and Saccharomyces cerevisiae accumulated zinc from zinc-containing medium. Uptake was biphasic and consisted of an initial, rapid, metabolism-independent binding of zinc to cell surfaces which was followed by slower, metabolism-dependent intracellular uptake of zinc. Spor. roseus could bind approximately eight times more zinc, per unit surface area, than could S. cerevisiae. Metabolism-dependent zinc uptake followed Michaelis-Menten kinetics with K m values of 0.09 and 5.00 mM-Zn2+ for Spor. roseus and S. cerevisiae, respectively; corresponding V max values were 0.51 and 9.09 nmol Zn2+ (mg dry wt)−1 min−1. Zinc uptake by viable cells was not accompanied by potassium release in either yeast, but zinc levels which affected viability in S. cerevisiae caused this yeast to release K+. No efflux of K+ was observed for Spor. roseus despite its greater sensitivity to zinc.