Liquid medium supplemented with 35% (w/v) PEG supported regeneration of the cell wall in protoplasts of Saccharomyces cerevisiae. The regeneration was followed by reversion to normal cells. Both these morphogenetic processes were similar to those described previously in gelatin media. The frequency of reversion was estimated to be 20 to 30%. The regeneration medium with PEG also induced protoplast fusion. When complementary auxotrophs were used for fusion only the fused products proliferated, giving first regenerated protoplasts and then hybrid cells.
KopeckáM., FarkašV.1979; RNA synthesis and the formation of the cell wall. Effect of lomofungin on regenerating protoplasts of Saccharomyces cerevisiae. Journal of General Microbiology 110:453–463
KopeckáM., ČtvrtnxíčekO., NečasO.1967; Formation and properties of the fibrillar network formed in yeast protoplasts as the first step of biosynthesis of the cell wall. In Symposium über Hefeprotoplasten pp. 73–76MüllerR. Edited by Berlin: Akademie Verlag;
LeupoldU.1955; Versuche zur genetischen Klassi- fizierung adeninabhängiger Mutanten von Schizo-saccharomyces pombe. Archiv der Julius Klaus-Stif-tung für Vererbungsforschung, Sozialanthropologie und Rassenhygiene 30:506–516
NečasO., SvobodaA.1981; Morphogenesis in protoplasts. In Yeast Cell Envelopes - Biochemistry, Biophysics, and Ultrastructure pp. 105–127ArnoldW.N. Edited by Boca Raton: CRC Press;
SvobodaA., NečasO.1970; Experimental decoupling in the synthesis of fibrillar and amorphous components during regeneration of the cell wall of Saccharomvces cerevisiae protoplasts. In Yeast Protoplasts pp. 211–215NečasO., SvobodaA. Edited by Brno: J. E. Purkyne University;