1887

Abstract

Summary: Liquid medium supplemented with 35% (w/v) PEG supported regeneration of the cell wall in protoplasts of The regeneration was followed by reversion to normal cells. Both these morphogenetic processes were similar to those described previously in gelatin media. The frequency of reversion was estimated to be 20 to 30%. The regeneration medium with PEG also induced protoplast fusion. When complementary auxotrophs were used for fusion only the fused products proliferated, giving first regenerated protoplasts and then hybrid cells.

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/content/journal/micro/10.1099/00221287-129-11-3371
1983-11-01
2019-10-22
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http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-129-11-3371
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