1887

Abstract

Liquid medium supplemented with 35% (w/v) PEG supported regeneration of the cell wall in protoplasts of The regeneration was followed by reversion to normal cells. Both these morphogenetic processes were similar to those described previously in gelatin media. The frequency of reversion was estimated to be 20 to 30%. The regeneration medium with PEG also induced protoplast fusion. When complementary auxotrophs were used for fusion only the fused products proliferated, giving first regenerated protoplasts and then hybrid cells.

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-129-11-3371
1983-11-01
2022-01-17
Loading full text...

Full text loading...

/deliver/fulltext/micro/129/11/mic-129-11-3371.html?itemId=/content/journal/micro/10.1099/00221287-129-11-3371&mimeType=html&fmt=ahah

References

  1. Ballou C. 1976; Structure and biosynthesis of the mannan component of the yeast cell envelope. Advances in Microbial Physiology 14:93–158
    [Google Scholar]
  2. Carpita N.C., Delmer D.P. 1980; Protection of cellulose synthesis! in detached cotton fibers by polyethylene glycol. Plant Physiology 66:911–916
    [Google Scholar]
  3. Chung C.W., Nickerson W.J. 1954; Polysaccharide synthesis in growing yeasts. Journal of Biological Chemistry 208:395–407
    [Google Scholar]
  4. Darling S. 1976; Polyethylene glycol-induced reversion of yeast protoplasts in liquid medium. Yeast Newsletter 25:5–6
    [Google Scholar]
  5. Eddy A.A., Williamson D.H. 1959; Formation of aberrant cell walls and of spores by growing yeast protoplasts. Nature; London: 1831101–1104
    [Google Scholar]
  6. Kopecká M., Farkaš V. 1979; RNA synthesis and the formation of the cell wall. Effect of lomofungin on regenerating protoplasts of Saccharomyces cerevisiae. Journal of General Microbiology 110:453–463
    [Google Scholar]
  7. Kopecká M., Čtvrtnxíček O., Nečas O. 1967; Formation and properties of the fibrillar network formed in yeast protoplasts as the first step of biosynthesis of the cell wall. In Symposium über Hefeprotoplasten pp. 73–76 Müller R. Edited by Berlin: Akademie Verlag;
    [Google Scholar]
  8. Lampen J.O. 1968; External enzymes of yeast: their nature and formation. Antonie van Leeuwenhoek 34:1–18
    [Google Scholar]
  9. Leupold U. 1955; Versuche zur genetischen Klassi- fizierung adeninabhängiger Mutanten von Schizo-saccharomyces pombe. Archiv der Julius Klaus-Stif-tung für Vererbungsforschung, Sozialanthropologie und Rassenhygiene 30:506–516
    [Google Scholar]
  10. Moor H., Mühlethaler K. 1963; Fine structure of frozen-etched yeast cells. Journal of Cell Biology 17:609–628
    [Google Scholar]
  11. Nečas O. 1961; Physical conditions as important factors for the regeneration of naked yeast protoplasts. Nature; London: 1921664–1665
    [Google Scholar]
  12. Nečas O. 1971; Cell wall synthesis in yeast protoplasts. Bacteriological Reviews 35:149–170
    [Google Scholar]
  13. Nečas O., Svoboda A. 1976; Regeneration of yeast protoplasts. A freeze-etching study. Zeitschrift für allgemeine Mikrobiologie 16:615–625
    [Google Scholar]
  14. Nečas O., Svoboda A. 1981; Morphogenesis in protoplasts. In Yeast Cell Envelopes - Biochemistry, Biophysics, and Ultrastructure pp. 105–127 Arnold W.N. Edited by Boca Raton: CRC Press;
    [Google Scholar]
  15. Svoboda A. 1966; Regeneration of yeast protoplasts in agar gels. Experimental Cell Research 44:640–642
    [Google Scholar]
  16. Svoboda A. 1978; Fusion of yeast protoplasts induced by polyethylene glycol. Journal of General Microbiology 109:169–175
    [Google Scholar]
  17. Svoboda A., Nečas O. 1970; Experimental decoupling in the synthesis of fibrillar and amorphous components during regeneration of the cell wall of Saccharomvces cerevisiae protoplasts. In Yeast Protoplasts pp. 211–215 Nečas O., Svoboda A. Edited by Brno: J. E. Purkyne University;
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-129-11-3371
Loading
/content/journal/micro/10.1099/00221287-129-11-3371
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error