1887

Abstract

Gene fusions between the structural genes and various genes of the hexuronate system of K12 were isolated by the technique of Casadaban. Mud(Ap ) and p-Mu insertion mutants were constructed in which the genes were fused to the regulatory region of the uxu operon. In all the fusion strains, -galactosidase expression was shown to be inducible by the natural inducers of the operon (glucuronate and fructuronate) and sensitive to catabolite repression by glucose. In addition we isolated a Mud(Ap ) fusion where the genes were fused to the regulatory gene. In this fusion the synthesis of -galactosidase reflects the regulation of the gene. In the presence of a wild-type allele, partial repression of -galactosidase expression was found; the repression was removed when inducer was added. This result indicates that while the gene is subject to autogenous control, the repressor may have only a low affinity for its own operator.

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1983-11-01
2024-04-18
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References

  1. Ashwell G. 1962; Enzymes of glucuronic and galacturonic acid metabolism in bacteria. Methods in Enzymology 5:190–208
    [Google Scholar]
  2. Bachmann J.B., Low K.B. 1980; Linkage map of Escherichia coli K-12, edition 6. Microbiological Reviews 44:1–56
    [Google Scholar]
  3. Brent R., Ptashne M. 1980; The lexA gene product represses its own promoter. Proceedings of the National Academy of Sciences of the United States of America 77:1932–1936
    [Google Scholar]
  4. Bukhari A.I., Ljungquist E. 1977; Bacteriophage Mu: methods for cultivation and use. In DNA Insertion Elements, Plasmids and Episomes pp. 749–756 Bukhari A.I., Shapiro J.A., Adhya S.L. Edited by New York:: Cold Spring Harbor Laboratory.;
    [Google Scholar]
  5. Camakaris H., Pittard J. 1982; Autoregulation of the tyrR gene. Journal of Bacteriology 150:70–75
    [Google Scholar]
  6. Casadaban M.J. 1976a; Transposition and fusion of the lac genes to selected promoters in Escherichia coliusing bacteriophage lambda and Mu. Journal of Molecular Biology 104:541–555
    [Google Scholar]
  7. Casadaban M.J. 1976b; Regulation of the regulatory gene for the arabinose pathway araC. Journal of Molecular Biology 104:557–566
    [Google Scholar]
  8. Casadaban M.J., Cohen S.N. 1979; Lactose genes fused to exogenous promoters in one step using a Mu-/ac bacteriophage: in vivo probe for transcriptional control sequences. Proceedings of the National Academy of Sciences of the United States of America 76:4530–4533
    [Google Scholar]
  9. DÉbarbouillÉ M., Schwartz M. 1979; The use of gene fusions to study the expression of malT, the positive regulator gene of the maltose regulon. Journal of Molecular Biology 132:521–534
    [Google Scholar]
  10. Gunsalus R.P., Yanofsky C. 1980; Nucleotide sequence and expression of Escherichia coli trpR,the structural gene for the trpaporepressor. Proceedings of the National Academy of Sciences of the United States of America 77:7117–7121
    [Google Scholar]
  11. Howe M.M., Bade E.G. 1975; Molecular biology of bacteriophage Mu. Science 190:624–632
    [Google Scholar]
  12. Hugouvieux-Cotte-Pattat N., Robert-Baudouy J. 1981; Isolation of fusions between the lac genes and several genes of the exuregulon: analysis of their regulation, determination of the transcription direction of the uxaC-uxaA operon, in Escherichia coli K- 12. Molecular and General Genetics 182:279–287
    [Google Scholar]
  13. Hugouvieux-Cotte-Pattat N., Robert-Baudouy J. 1982; Control of exuR, a negative regulator gene of the hexuronate system in Escherichia coli K-12. Journal of Molecular Biology 156:221–228
    [Google Scholar]
  14. Komeda Y., Iino T. 1979; Regulation of expression of the flagellin gene (hag) in Escherichia coli K- 12: analysis of hag-lac fusions. Journal of Bacteriology 139:721–729
    [Google Scholar]
  15. Miller J.H. 1972 Experiments in Molecular Genetics. New York:: Cold Spring Harbor Laboratory.;
    [Google Scholar]
  16. Novel M., Novel G. 1976; Regulation of β-glucuronidase synthesis in Escherichia coliK-12: pleiotropic constitutive mutations affecting uxu and uidA expression. Journal of Bacteriology 127:418–432
    [Google Scholar]
  17. Portalier R., Stoeber F. 1972; Dosages colorimétriques des oxydoréductases aldoniques d’Escherichia coli K-12: applications. Biochimica et biophysica acta 289:19–27
    [Google Scholar]
  18. Portalier R., Robert-Baudouy J., Stoeber F. 1980; Regulation of the hexuronate system genes in Escherichia coli K-12: the exuregulon. Journal of Bacteriology 143:1095–1107
    [Google Scholar]
  19. Ritzenthaler P., Mata-Gilsinger M. 1982; Use of in vitro gene fusions to study the uxuR regulatory gene in Escherichia coli K-12: direction of transcription and regulation of its expression. Journal of Bacteriology 150:1040–1047
    [Google Scholar]
  20. Ritzenthaler P., Mata-Gilsinger M. 1983; Use of uxu-lac fusion strains to study the regulation of the uxuAB operon in Escherichia coli. Journal of General Microbiology 129:3335–3343
    [Google Scholar]
  21. Robert-Baudouy J., Portalier R. 1974; Mutations affectant le catabolisme du glucuronate chez Escherichia coli K-12. Molecular and General Genetics 131:31–46
    [Google Scholar]
  22. Robert-Baudouy J., Stoeber F. 1973; Purification et propriétés de la d-mannonate hydrolyase d’Escherichia coli. Biochimica et biophysica acta 309:474–485
    [Google Scholar]
  23. Robert-Baudouy J., Portalier R., Stoeber F. 1974; Régulation du métabolisme des hexuronates chez Escherichia coli K-12: modalités de l’induction des enzymes du système hexuronate. European Journal of Biochemistry 43:1–15
    [Google Scholar]
  24. Robert-Baudouy J., Portalier R., Stoeber F. 1981; Regulation of hexuronate system genes in Escherichia coli K-12: multiple regulation of the uxuoperon by the exuR and uxuR gene products. Journal of Bacteriology 145:211–220
    [Google Scholar]
  25. Smith G.R., Magasanik B. 1971; Nature and selfregulated synthesis of the repressor of the hutoperons in Salmonella typhimurium. Proceedings of the National Academy of Sciences of the United States of America 68:1493–1497
    [Google Scholar]
  26. Van Gijsegem F., Toussaint A. 1982; Chromosome transfer and R-prime formation by an RP4:: mini-Mu derivative in Escherichia coli, Salmonella typhimurium, Klebsiellapneumoniae and Proteus mirabilis. Plasmid 7:30–44
    [Google Scholar]
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