1887

Abstract

In the hexuronate system of , the operon is negatively controlled by the UxuR and ExuR repressors. A Mud phage was used, by the method of Casadaban, to construct strains where a truncated was fused to From these fusion strains, deletions of various lengths extending into the region were created by selecting temperature-insensitive mutants. Operator-constitutive mutations were also selected for in such strains and their preliminary analysis is presented. Large amounts of either ExuR or UxuR repressor caused a strong decrease of the constitutive expression of the operon in the operator mutants. The implications of this repression for the presence of one or two operator sites in the operon are discussed.

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1983-11-01
2024-04-19
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References

  1. Ashwell G. 1962; Enzymes of glucuronic and galacturonic acid metabolism in bacteria. Methods in Enzymology 5:190–208
    [Google Scholar]
  2. Bachmann B.J. 1983; Linkage map of Escherichia coli K-12, edition 7. Microbiological Reviews 47:180–230
    [Google Scholar]
  3. Beny G., Boyen A., Charlier D., Lissens W., Feller A., Glansdorff N. 1982; Promoter mapping and selection of operator mutants by using insertion of bacteriophage Mu in the argECBH divergent operon of Escherichia coli K-12. Journal of Bacteriology 151:62–67
    [Google Scholar]
  4. Bukhari A.I. 1975; Reversal of mutator phage Mu integration. Journal of Molecular Biology 96:87–99
    [Google Scholar]
  5. Bukhari A.I. 1976; Bacteriophage Mu as a transposition element. Annual Review of Genetics 10:389–412
    [Google Scholar]
  6. Casadaban M.J. 1976; Transposition and fusion of the lac genes to selected promoters in Escherichia coli using bacteriophage lambda and Mu. Journal of Molecular Biology 104:541–555
    [Google Scholar]
  7. Casadaban M.J., Cohen S.N. 1979; Lactose genes fused to exogenous promoters in one step using a Mu-lac bacteriophage: in vivo probe for transcriptional control sequences. Proceedings of the National Academy of Sciences of the United States of America 76:4530–4533
    [Google Scholar]
  8. Chang A.C.Y., Cohen S.N. 1978; Construction and characterization of amplifiable multicopy DNA cloning vehicles derived from the P15A cryptic miniplasmid. Journal of Bacteriology 134:1141–1156
    [Google Scholar]
  9. Faelen M., Toussaint A. 1980; Inversion induced by temperate bacteriophage Mu-1 in the chromosome of Escherichia coli K-12. Journal of Bacteriology 142:391–399
    [Google Scholar]
  10. Low K.B. 1972; Escherichia coli K-12 F-prime factors: old and new. Bacteriological Reviews 36:587–607
    [Google Scholar]
  11. Mandel M., Higa H. 1970; Calcium-dependent bacteriophage DNA infection. Journal of Molecular Biology 53:159–162
    [Google Scholar]
  12. Miller J.H. 1972 Experiments in Molecular Genetics New York: Cold Spring Harbor Laboratory;
    [Google Scholar]
  13. Mulligan J.T., Margolin W., Krueger J.H., Walker G.C. 1982; Mutations affecting regulation of methionine biosynthetic genes isolated by use of met-lac fusions. Journal of Bacteriology 151:609–619
    [Google Scholar]
  14. Novel G., Didier-Fichet M.L., Stoeber F. 1974; Inducibility of β-glucuronidase in wild-type and hexuronate-negative mutants of Escherichia coli K-12. Journal of Bacteriology 120:89–95
    [Google Scholar]
  15. Novel M., Novel G. 1976a; Regulation of β- glucuronidase synthesis in Escherichia coli K-12: constitutive mutants specially derepressed for uidA expression. Journal of Bacteriology 127:406–417
    [Google Scholar]
  16. Novel M., Novel G. 1976b; Regulation of β- glucuronidase synthesis in Escherichia coli K-12: pleiotropic constitutive mutations affecting uxu and uidA expression. Journal of Bacteriology 127:418–432
    [Google Scholar]
  17. Portalier R., Stoeber F. 1972a; Dosages colorimetriques des oxydoréductases aldoniques d‘E. coli K-12: applications. Biochimica et biophysica acta 289:19–27
    [Google Scholar]
  18. Portalier R., Stoeber F. 1972b; Lad-manno- nate:NAD+ oxydoreductase d‘Escherichia coli K-12: purification, propriétés et individualité. European Journal of Biochemistry 26:290–300
    [Google Scholar]
  19. Portalier R., Robert-Baudouy J., Stoeber F. 1980; Regulation of Escherichia coli K-12 hexuron- ate system genes: exu regulon. Journal of Bacteriology 143:1095–1107
    [Google Scholar]
  20. Ritzenthaler P., Mata-Gilsinger M. 1982; Use of in vitro gene fusions to study the uxuR regulatory gene in Escherichia coli K-12: direction of transcription and regulation of its expression. Journal of Bacteriology 150:1040–1047
    [Google Scholar]
  21. Ritzenthaler P., Mata-Gilsinger M., Stoeber F. 1980; Construction and expression of hybrid plasmids containing Escherichia coli K-12 uxu genes. Journal of Bacteriology 143:1116–1126
    [Google Scholar]
  22. Ritzenthaler P., Mata-Gilsinger M., Stoeber F. 1981; Molecular cloning of Escherichia coli K-12 hexuronate system genes: exu region. Journal of Bacteriology 145:181–190
    [Google Scholar]
  23. Ritzenthaler P., Blanco C., Mata-Gilsinger M. 1983; Interchangeability of repressors for the control of the uxu and uid operons in E. coli K-12. Molecular and General Genetics 191:263–270
    [Google Scholar]
  24. Robert-Baudouy J., Stoeber F. 1973; Purification et propriétés de lad-mannonate hydrolyase d‘Escherichia coli. . Biochimica et biophysica acta 309:473–485
    [Google Scholar]
  25. Robert-Baudouy J., Portalier R., Stoeber F. 1974; Regulation du metabolisme des hexuronates chez Escherichia coli K-12: modalites de l‘induction des enzymes du systeme hexuronate. European Journal of Biochemistry 43:1–15
    [Google Scholar]
  26. Robert-Baudouy J., Portalier R., Stoeber F. 1981; Regulation of hexuronate system genes in Escherichia coli K-12: multiple regulation of the uxu operon by exuR and uxuR gene products. Journal of Bacteriology 145:211–220
    [Google Scholar]
  27. Van De Putte P.G., Westmaas G., Giphart M., Wijffelman C. 1977; On the kil gene of bacteriophage Mu. In DNA Insertion Elements, Plasmids and Episomes pp. 287–294 Edited by Bukhari A.I., Shapiro J.A., Adhya S.L. New York: Cold Spring Harbor Laboratory;
    [Google Scholar]
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