Summary: In the hexuronate system of the operon is negatively controlled by the UxuR and ExuR repressors. A Mud phage was used, by the method of Casadaban, to construct strains where a truncated was fused to From these fusion strains, deletions of various lengths extending into the region were created by selecting temperature-insensitive mutants. Operator-constitutive mutations were also selected for in such strains and their preliminary analysis is presented. Large amounts of either ExuR or UxuR repressor caused a strong decrease of the constitutive expression of the operon in the operator mutants. The implications of this repression for the presence of one or two operator sites in the operon are discussed.


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