In contrast to most yeasts, which ferment glucose more rapidly than fructose, Saccharomyces bailii ferments fructose first, then glucose. Thus, in a medium containing fructose and glucose, diauxic growth results. Cells of S. bailii that were grown on fructose were unable to ferment glucose when suspended in a glucose-containing buffer solution. Fructose-grown cells were cryptic for glucose fermentation but contained the enzymes for glucose metabolism. When suspended for 2 h in a growth medium containing glucose, fructose-grown cells acquired the ability to ferment glucose, due to the synthesis of a carrier protein. This induction was prevented by cycloheximide. In S. bailii, fructose was transported into the cells by a constitutive carrier system that was insensitive to uranyl ions. The inducible glucose carrier system was completely inhibited by 10−4 M-uranyl ions. If subsequent metabolism of hexoses was inhibited by iodoacetic acid, the uptake of hexoses could be measured by the increase in their intracellular concentrations. Fructose-grown cells took up only fructose whereas glucose-grown cells possessed an inducible glucose carrier and uptake of both glucose and fructose was observed. A model is proposed to explain the sequential fermentation of fructose and glucose in S. bailii.
BeckerJ.U.,
BetzA.1972; Membrane transport as controlling pacemaker of glycolysis in Saccharomyces carlsbergensis. Biochimica et biophysica acta 274:584–597
BergmeyerH.U.,
BerntE.,
SchmidtF.,
StorkH.1974; d-Glucose: Bestimmungmit Hexokinase und Glucose-6-phosphat-Dehydrogenase. In Methoden der enzymatischen Analyse2 pp. 1241–1246BergmeyerH.U.
Edited by Weinheim:: Verlag Chemie.;
BerntE.,
BergmeyerH.U.1974; d-Fructose: Bestimmungmit Hexokinase, Glucose-6-phosphat-Dehydrogenase und Phosphoglucose-Isomerase. In Methoden der enzymatischenAnalyse2 pp. 1349–1352BergmeyerH.U.
Edited by Weinheim:: Verlag Chemie.;
CirilloV.P.1968; Relationship between sugar structure and competition for the sugar transport system in baker’s yeast. Journal of Bacteriology 95:603–611
DubrunfautM.1847; Sur une propriété analytique des fermentations alcoolique et lactique, et surleur application á l’étude des sucres. Annales de chimie et de physique 3:167–178
KotykA.,
MichaljanicovaD.1968; Properties of the sugar carrier in baker’s yeast. IV. An asymmetric component of monosaccharide transport. Folia microbiologica 13:212–220
KuczynskiJ.T.,
RadlerF.1982; The anaerobic metabolism of malate of Saccharomyces bailii and the partial purification and characterization of malic enzyme. Archives of Microbiology 131:266–270
RomanoA.H.1982; Facilitated diffusion of 6-deoxy-d-glucose in baker’s yeast: evidence against phosphorylation-associated transport of glucose. Journal of Bacteriology 152:1295–1297
SolsA.1968; Regulation of carbohydrate transport and metabolism in yeast. In Aspects of Yeast Metabolism pp. 47–66MillsA.K.
Edited by Oxford & Edinburgh:: Blackwell Scientific Publications.;
Van SteveninckJ.1969; The mechanisms of transmembrane glucose transport in yeast: evidence for phosphorylation, associated with transport. Archives of Biochemistry 130:244–252
TilburyR.H.1980; Xerotolerant (osmophilic) yeast. In Biology and Activities of Yeast pp. 153–179SkinnerF.A.,
PassmoreS.M.,
DavenportR.R.
Edited by London:: Academic Press.;