RT Journal Article SR Electronic(1) A1 Van Laere, andr J.YR 1983 T1 Stimulation of Phosphofructokinase from Phycomyces blakesleeanus and Some Other Fungi by Micromolar Concentrations of Fructose 2,6-bisphosphate JF Microbiology, VO 129 IS 10 SP 3281 OP 3285 DO https://doi.org/10.1099/00221287-129-10-3281 PB Microbiology Society, SN 1465-2080, AB The phosphofructokinase in crude extracts of Phycomyces blakesleeanus required the presence of ammonium salts, AMP or fructose 2,6-bisphosphate for its activity. The enzyme had slightly sigmoidal kinetics with respect to fructose 6-phosphate as substrate. It was slightly inhibited by high ATP concentrations and by citrate. Fructose 2,6-bisphosphate stimulated the Phycomyces blakesleeanus phosphofructokinase; the K m for fructose 6-phosphate decreased, the inhibition by ATP was completely relieved and the affinity for the activator ammonia was increased. AMP also stimulated the catalytic activity but there was poor co-operation with fructose 2,6-bisphosphate. Preliminary experiments showed that fructose 2,6-bisphosphate also stimulated the phosphofructokinase from Mucor rouxii, Neurospora tetrasperma and Agaricus bisporus. , UL https://www.microbiologyresearch.org/content/journal/micro/10.1099/00221287-129-10-3281