1887

Abstract

Summary: The phosphofructokinase in crude extracts of required the presence of ammonium salts, AMP or fructose 2,6-bisphosphate for its activity. The enzyme had slightly sigmoidal kinetics with respect to fructose 6-phosphate as substrate. It was slightly inhibited by high ATP concentrations and by citrate. Fructose 2,6-bisphosphate stimulated the phosphofructokinase; the for fructose 6-phosphate decreased, the inhibition by ATP was completely relieved and the affinity for the activator ammonia was increased. AMP also stimulated the catalytic activity but there was poor co-operation with fructose 2,6-bisphosphate. Preliminary experiments showed that fructose 2,6-bisphosphate also stimulated the phosphofructokinase from and

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/content/journal/micro/10.1099/00221287-129-10-3281
1983-10-01
2020-01-22
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http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-129-10-3281
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