1887

Abstract

The genes and encoding the first two enzymes of the proline biosynthetic sequence in Escherichia were subcloned from a ColE1 hybrid plasmid containing 23·3 kilobases of genomic DNA. and are contiguous and constitute a single operon transcribed in the direction . The operon is contiguous with the gene . Hybridization experiments showed no homology between of and the other regions of the genome or with the DNA of several other bacterial species.

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1983-10-01
2021-10-28
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References

  1. Bachmann B.J., Low K.B. 1980; Linkage map of Escherichia coli K-12, . , edition 6.. Microbiological Reviews 44:1–56
    [Google Scholar]
  2. Baich A. 1969; Proline synthesis in Escherichia coli.A proline-inhibitible glutamic acid kinase. Biochi-mica et biophysica acta 192:462–467
    [Google Scholar]
  3. Baich A., Pierson D.J. 1965; Control of proline biosynthesis in Escherichia coli. Biochimica et biophysica acta 104:397–404
    [Google Scholar]
  4. Birnboim M.C., Doly J. 1979; A rapid alkaline extraction procedure for screening recombinant plasmid DNA. Nucleic Acids Research 7:1513–1524
    [Google Scholar]
  5. Broda P. 1974; Modified map positions for lac and pro markers in Escherichia coli K12. Journal of Bacteriology 117:741–746
    [Google Scholar]
  6. Clarke L. 1976; Colony bank containing synthetic ColEl hybrid plasmids representative of the entire E. coli genome. Cell 9:91–99
    [Google Scholar]
  7. Condamine H. 1971; Mutants des voies de biosynth-ese et de dégradation de la proline chez Escherichia coli (K12). Annales de l’Institut Pasteur 120:9–22
    [Google Scholar]
  8. Edlund T., Grundstrom T., Normark S. 1979; Isolation and characterization of DNA repetitions carrying the chromosomal β-lactamase gene of Escherichia coli K-12. Molecular and General Genetics 173:115–125
    [Google Scholar]
  9. Hayzer D.J., Leisinger TH. 1980; The gene-enzyme relationships of proline biosynthesis in Escherichia coli. . Journal of General Microbiology 118:287–293
    [Google Scholar]
  10. Hayzer D.J., Leisinger TH. 1982; Proline biosynthesis in Escherichia coli: purification and characterisation of glutamate semialdehyde dehydrogenase. European Journal of Biochemistry 121:561–565
    [Google Scholar]
  11. Humphreys G.O., Willshaw G.A., Anderson E.S. 1975; A simple method for the preparation of large quantities of pure plasmid DNA. Biochimica et biophysica acta 383:457–463
    [Google Scholar]
  12. Johnson J.L. 1981; Genetic characterisation. In Manual of Methods of General Bacteriology p. 452 Washington, D.C.: American Society of Microbiology;
    [Google Scholar]
  13. Krishna R.V., Leisinger TH. 1979; Biosynthesis of proline in Pseudomonas aeruginosa. Partial purification and characterisation of γ-glutamyl kinase. Biochemical Journal 181:215–222
    [Google Scholar]
  14. Krishna R.V., Beilstein P., Leisinger TH. 1979; Biosynthesis of proline in Pseudomonas aeruginosa. Properties of γ-glutamyl phosphate reductase and l-pyrroline-5-carboxylate reductase. Biochemical Journal 181:223–230
    [Google Scholar]
  15. Lerudulier D., Valentine R.C. 1982; Genetic engineering in agriculture: osmoregulation. Trends in Biochemical Sciences 7:431–433
    [Google Scholar]
  16. Menslee J.C., Jones M.E. 1982; Ornithine synthesis from glutamate in rat small intestinal mucosa. Archives of Biochemistry and Biophysics 219:186–197
    [Google Scholar]
  17. Miller J.H. 1972 Experiments in Molecular Genetics. Cold Spring Harbor, New York: Cold Spring Harbor Laboratory;
    [Google Scholar]
  18. Pardee A.B., Prestidge L.S. 1961; The initial kinetics of enzyme induction. Biochimica et biophysica acta 49:77–88
    [Google Scholar]
  19. Pemberton J., Holloway B.W. 1972; Chromosome mapping in Pseudomonas aeruginosa. . Genetical Research 19:251–260
    [Google Scholar]
  20. Roberts L.M., Reeve E.C.R. 1970; Two mutations giving low level streptomycin resistance in Escherichia coli K-12. Genetical Research 16:359–365
    [Google Scholar]
  21. Royle P.L., Matsumoto H., Holloway B.W. 1981; Genetic circularity of the Psuedomonas aeruginosa PAO chromosome. Journal of Bacteriology 145:145–155
    [Google Scholar]
  22. Sanderson K.E., Hartman P.E. 1978; Linkage map of Salmonella typhimurium. . Microbiological Reviews 43:471
    [Google Scholar]
  23. Southern E.M. 1975; Detection of specific sequences among DNA fragments separated by gel electrophoresis. Journal of Molecular Biology 98:503–517
    [Google Scholar]
  24. Sutcliffe J.G. 1978; Complete nucleotide sequence of the Escherichia coli plasmid pBR322. Cold Spring Harbor Symposium on Quantitative Biology 43:77–90
    [Google Scholar]
  25. Thomas P.S. 1980; Hybridisation of denatured RNA and small DNA fragments transferred to nitrocellulose. Proceedings of the National Academy of Sciences of the United States of America 77:5201–5205
    [Google Scholar]
  26. Tommassen J., Lugtenberg B. 1981; Localisation of phoE, the structural gene for outer membrane protein e in Escherichia coli K12. Journal of Bacteriology 147:118–123
    [Google Scholar]
  27. Tommassen J., Van Der Ley P., Lugtenberg B. 1981; Genetic and biochemical characterisation of an Escherichia coli K-12 mutant with an altered outer membrane protein. Antonie van Leeuwenhoek 47:325–337
    [Google Scholar]
  28. Tommassen J., Overduir P., Lugtenberg B., Bergmans H. 1982; Cloning ofphoE, the structural gene for the Escherichia coli phosphate limitation- inducible outer membrane pore protein. Journal of Bacteriology 149:668–672
    [Google Scholar]
  29. Vogel H.J., Davies B.D. 1952; Glutamic γ- semialdehyde and △1-pyrroline-5-carboxylic acid, intermediates in the biosynthesis of proline. Journal of the American Chemical Society 74:109–112
    [Google Scholar]
  30. Yanofsky C. 1981; Attenuation in the control of expression of bacterial operons. Nature; London: 289751–758
    [Google Scholar]
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