Summary: Plasmid pHM2 contains a gene and is able to replicate in both and The plasmid was mobilized at low frequency by the F plasmid. Nearly 30% of the mobilized plasmids contained an insert of Tn1000 (γδ). Fourteen of the inserts were in the DNA portion of pHM2. Of these, two adjacent inserts abolished expression of the plasmid gene in From the map positions of flanking inserts that do not abolish gene expression, it is estimated that the gene is probably not more than 700 bp long.


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