1887

Abstract

The outer membrane of strain 3283-61 (serotype O2:K3) was isolated from blebs released upon spheroplast formation, in the presence of lysozyme and EDTA, by isopycnic sucrose density gradient centrifugation. SDS-PAGE of the outer membrane fraction prepared from cells grown in nutrient broth containing 3% (w/v) NaCl revealed five major proteins, designated a to e, with apparent approximate molecular weights: a, 44000; b, 36 000; c, 33500; d, 26500; e, 22000. An increase in NaCl concentration in the growth medium resulted in an increase of proteins b and c, whereas a decrease to 0·5% (w/v) induced two additional major proteins with respective molecular weights of about 35000 and 32000. Proteins a and b appeared to be loosely associated with the peptidoglycan layer since they were largely retained after extraction with 2% (w/v) SDS at 50 C for 30 min. Proteins c and/or e may play a role in phage VP1-receptors since phage-resistant mutants derived from strain 3283-61 had significantly diminished amounts of both proteins. The major outer membrane proteins varied in number and molecular weight in strains of belonging to different K-serotypes.

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-129-10-3185
1983-10-01
2021-10-25
Loading full text...

Full text loading...

/deliver/fulltext/micro/129/10/mic-129-10-3185.html?itemId=/content/journal/micro/10.1099/00221287-129-10-3185&mimeType=html&fmt=ahah

References

  1. Bassford P.J.JR, Schnaitman C.L., Reeves P. 1977; Outer membrane proteins of Escherichia coli. VI. Protein alterations in bacteriophage-resistant mutants. Journal of Bacteriology 131:608–622
    [Google Scholar]
  2. Beuchat L.R., Worthington R.E. 1976; Relationships between heat resistance and phospholipid fatty acid composition of Vibrio parahaemolyticus. Applied and Environmental Microbiology 31:389–394
    [Google Scholar]
  3. Blake P.A., Weaver R.E., Hollis D.G. 1980; Diseases of human (other than cholera) caused by vibrios. Annual Review of Microbiology 34:341–367
    [Google Scholar]
  4. Buckley J.T., Howard S.P., Trust T.J. 1981; Influence of virulence plasmid and geographic origin on outer membrane proteins of Vibrio anguillarum. FEMS Microbiology Letters 11:41–46
    [Google Scholar]
  5. Crosa J.H., Hodges L.L. 1981; Outer membrane proteins induced under conditions of iron limitation in the marine fish pathogen Vibrio anguillarum. Infection and Immunity 31:223–227
    [Google Scholar]
  6. Deneke C.F., Colwell R.R. 1973; Studies of the cell envelope of Vibrio parahaemolyticus. Canadian Journal of Microbiology 19:241–245
    [Google Scholar]
  7. Fairbanks G., Steck T.L., Wallach D.H.F. 1971; Electrophoretic analysis of the major poly-peptides of the human erythrocyte membrane. Biochemistry 10:2606–2617
    [Google Scholar]
  8. Hantke K. 1978; Major outer membrane proteins of Escherichia coli K12 serve as receptors for the phages T2 (protein la) and 434 (protein lb). Molecular and General Genetics 164:131–135
    [Google Scholar]
  9. Hasin M., Rottem S., Razin S. 1975; The outer membrane of Proteus mirabilis. I. Isolation and characterization of the outer and cytoplasmic membrane fractions. Biochimica et biophysica acta 375:381–394
    [Google Scholar]
  10. Henning U., Haller I. 1975; Mutants of Escherichia coli K12 lacking all “major” proteins of the outer cell envelope membrane. FEBS Letters 55:161–164
    [Google Scholar]
  11. Henning U., Jann K. 1979; Two-component nature of bacteriophage T4 receptor activity in Escherichia coli K-12. Journal of Bacteriology 137:664–666
    [Google Scholar]
  12. Hiramatsu T., Yano I., Masui M. 1980; Effect of NaCl concentration on the protein species and phospholipid composition of the outer membrane in a moderately halophilic bacterium. FEMS Microbiology Letters 7:289–292
    [Google Scholar]
  13. Hisatsune K., Kiuye A., Kondo S. 1980; Sugar composition of O-antigenic lipopolysaccharides isolated from Vibrio parahaemolyticus. Microbiology and Immunology 24:691–701
    [Google Scholar]
  14. Hisatsune K., Kiuye A., Kondo S. 1981; A comparative study of the sugar composition of O- antigenic lipopolysaccharides isolated from Vibrio alginolyticus and Vibrio parahaemolyticus. Microbiology and Immunology 25:127–136
    [Google Scholar]
  15. Hofstra H.H., Van Tol M.J.D., Dankert J. 1980; Cross-reactivity of major outer membrane proteins of Enterobacteriaceae, studied by crossed immunoelectrophoresis. Journal of Bacteriology 143:328–337
    [Google Scholar]
  16. Kabir S. 1980; Composition and immunochemical properties of outer membrane proteins of Vibrio cholerae. Journal of Bacteriology 144:382–389
    [Google Scholar]
  17. Kellenberger E., Ryter A., Séchaud J. 1958; Electron microscope study of DNA-containing plasms. II. Vegetative and mature phage-DNA as compared with normal bacterial nucleoids in different physiological states. Journal of Biophysical and Biochemical Cytology 4:671–678
    [Google Scholar]
  18. Kelley J.T., Parker C.D. 1981; Identification and preliminary characterization of Vibrio choleraeouter membrane proteins. Journal of Bacteriology 145:1018–1024
    [Google Scholar]
  19. Koga T., Kawata T. 1981; Structure of a novel bacteriophage VP3 for Vibrio parahaemolyticus. Microbiology and Immunology 25:737–740
    [Google Scholar]
  20. Koga T., Toyoshima S., Kawata T. 1982; Morphological varieties and host ranges of Vibrio parahaemolyticus bacteriophages isolated from seawater. Applied and Environmental Microbiology 44:466–470
    [Google Scholar]
  21. Laemmli U.K. 1970; Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature, London 227:680–685
    [Google Scholar]
  22. Lugtenberg B., Bronstein H., Van Selm N., Peters R. 1977; Peptidoglycan-associated outer membrane proteins in gram negative bacteria. Biochimica et biophysica acta 465:571–578
    [Google Scholar]
  23. Manning P.A., Imbesi F., Haynes D.R. 1982; Cell envelope proteins in Vibrio cholerae. FEMS Microbiology Letters 14:159–166
    [Google Scholar]
  24. Masuda K., Kawata T. 1979; Ultrastructure and partial characterization of a regular array in the cell wall of Lactobacillus brevis. Microbiology and Immunology 23:941–953
    [Google Scholar]
  25. Matsushita K., Adachi O., Shinagawa E., Ameyama M. 1978; Isolation and characterization of outer and inner membranes from Pseudomonas aeruginosa and effect of EDTA on the membranes. Journal of Biochemistry 83:171–181
    [Google Scholar]
  26. Miwatani T., Takeda Y. 1976 Vibrio parahaemolyticus. A causative bacterium of food poisoning Tokyo: Saikon Publishing Co. Ltd;
    [Google Scholar]
  27. Mizuno T., Kageyama M. 1978; Separation and characterization of the outer membrane of Pseudomonas aeruginosa. Journal of Biochemistry 84:179–191
    [Google Scholar]
  28. Mizuno T., Kageyama M. 1979; Isolation and characterization of major outer membrane proteins of Pseudomonas aeruginosa strain PAO with special reference to peptidoglycan-associated protein. Journal of Biochemistry 86:979–989
    [Google Scholar]
  29. Mizushima S., Yamada H. 1975; Isolation and characterization of two outer membrane preparations from Escherichia coli. Biochimica et biophysica acta 375:44–53
    [Google Scholar]
  30. Mutoh N., Furukawa H., Mizushima S. 1978; Role of lipopolysaccharide and outer membrane protein of Escherichia coli K-12 in the receptor activity for bacteriophage T4. Journal of Bacteriology 136:693–699
    [Google Scholar]
  31. Nurminen M., Lounatmaa K., Sarvas M., Mäkela P.H., Nakae T. 1976; Bacteriophage- resistant mutants of Salmonella typhimurium deficient in two major outer membrane proteins. Journal of Bacteriology 127:941–955
    [Google Scholar]
  32. Osborn M.J., Gander J.E., Parisi E., Carson J. 1972; Mechanism of assembly of the outer membrane of Salmonella typhimurium. Isolation and characterization of cytoplasmic and outer membrane. Journal of Biological Chemistry 247:3962–3972
    [Google Scholar]
  33. Rosenbusch J.P. 1974; Characterization of the major envelope protein from Escherichia coli. Regular arrangement on the peptidoglycan and unusual dodecyl sulfate binding. Journal of Biological Chemistry 249:8019–8029
    [Google Scholar]
  34. Skurray R.A., Hancock R.E.W., Reeves P. 1974; Con mutants: class of mutants in Escherichia coli K-12 lacking a major cell wall protein and defective in conjugation and adsorption of a bacteriophage. Journal of Bacteriology 119:726–735
    [Google Scholar]
  35. Wolf-Watz H., Normark S., Bloom G.D. 1973; Rapid method for isolation of large quantities of outer membrane from Escherichia coli K-12 and its application to the study of envelope mutants. Journal of Bacteriology 115:1191–1197
    [Google Scholar]
  36. Yu F., Mizushima S. 1982; Roles of lipopolysaccharide and outer membrane protein OmpC of Escherichia coli K-12 in the receptor function for bacteriophage T4. Journal of Bacteriology 151:718–722
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-129-10-3185
Loading
/content/journal/micro/10.1099/00221287-129-10-3185
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error