1887

Abstract

The localization of the lectins (PA-I and PA-II) was studied using methods of osmotic shock, freezing and thawing and spheroplast formation. Very slight release of the two lectins occurred when was exposed to magnesium-osmotic shock or was frozen and thawed. Under these conditions, release of the periplasmic 5′-nucleotidase occurred, whereas no release of cytoplasmic glucose-6-phosphate dehydrogenase activity was detected. Formation of spheroplasts from by gradual removal of the bacterial envelopes revealed low lectin activity in the treatment fluids. Osmotic shock treatment of the lysozyme treated mureinoplasts resulted in low release of glucose-6-phosphate dehydrogenase and the two lectins (10–13%) and a considerable activity (38–4%) of 5′-nucleotidase. The presence of the lectins on the outer and the cytoplasmic membranes enabled intact cells and spheroplasts of to agglutinate papain-treated human eiythrocytes. These results indicate that the two lectins are located mainly in the cytoplasm with small fractions on the cytoplasmic and outer membranes and in the periplasmic space.

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1983-10-01
2024-04-24
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References

  1. Bhatti A.R., Devoe I.W., Ingram J.M. 1976; The release and characterization of some periplasm located enzymes of Pseudomonas aeruginosa. Canadian Journal of Microbiology 22:1425–1429
    [Google Scholar]
  2. Buchanan T.M., Pearce W.A. 1976; Pili as a mediator of the attachment of gonococci to human erythrocytes. In fection and Immunity 13:1483–1489
    [Google Scholar]
  3. Cheng K.-J., Ingram J.M., Costerton J.W. 1970; Release of alkaline phosphatase from cells of Pseudomonas aeruginosa by manipulation of cation concentration and pH. Journal of Bacteriology 104:748–753
    [Google Scholar]
  4. Cheng K.-J., Ingram J.M., Costerton J.W. 1971; Interactions of alkaline phosphatase and the cell wall of Pseudomonas aeruginosa. Journal of Bacteriology 107:325–336
    [Google Scholar]
  5. Cisar J.O., Barsumian E.L., Curl S.H., Vatter A.E., Sandberg A.L., Siraganian R.P. 1981; Detection and localization of a lectin on Actinomyces viscosus T14V by monoclonal antibodies. Journal of Immunology 127:1318–1322
    [Google Scholar]
  6. Collins F.M. 1964; Composition of cell walls of ageing Pseudomonas aeruginosa and Salmonella bethesda. Journal of General Microbiology 34:379–388
    [Google Scholar]
  7. Cumsky M., Zusman D. R. 1979; Myxobacterial hemagglutinin: a development-specific lectin of Myxococcus xanthus. Proceedings of the National Academy of Sciences of the United States of America 76:5505–5509
    [Google Scholar]
  8. Dasgupta B. R., Sugiyama H. 1977; Inhibition of Clostridium botulinum types A and B hemagglutinins by sugars. Canadian Journal of Microbiology 23:1257–1260
    [Google Scholar]
  9. Draper R.K., Chin D., Simon M.I. 1978; Diphtheria toxin has the properties of a lectin. Proceedings of the National Academy of Sciences of the United States of America 75:261–265
    [Google Scholar]
  10. Duguid J.P., Gillies R.R. 1957; Fimbriae and adhesive properties in dysentery bacilli. Journal of Pathology and Bacteriology 74:397–411
    [Google Scholar]
  11. Eshdat Y., Ofek I., Yashouv-Gan Y., Sharon N., Mirelman D. 1978; Isolation of a mannose-specific lectin from Escherichia coli and its role in the adherence of the bacteria to epithelial cells. Biochemical and Biophysical Research Communications 85:1551–1559
    [Google Scholar]
  12. Forsberg C.W., Costerton J.M., Macleod R.A. 1970; Separation and localization of cell wall layers of a Gram-negative bacterium. Journal of Bacteriology 104:1338–1353
    [Google Scholar]
  13. Fujita Y., Oishi K., Suzuki K., Imahori K. 1975; Purification and properties of an anti-B hemagglutinin produced by Streptomyces sp. Biochemistry 14:4465–4470
    [Google Scholar]
  14. Furth A.J. 1975; Purification and properties of a constitutive /1-lactamase from Pseudomonas aeruginosa strain Dalgleish. Biochimica et biophysica acta 377:431–443
    [Google Scholar]
  15. Garber N., Nachshon I. 1980; Localization of cholinesterase in Pseudomonas aeruginosa strain K. Journal of General Microbiology 117:279–283
    [Google Scholar]
  16. Garber N., Glick J., Gilboa-Garber N., Heller A. 1981; Interactions of Pseudomonas aeruginosa lectins with Escherichia coli strains bearing blood group determinants. Journal of General Microbiology 123:359–363
    [Google Scholar]
  17. Gilboa-Garber N. 1972a; Inhibition of broad spectrum hemagglutinin from Pseudomonas aeruginosa by d-galactose and its derivatives. FEBS Letters 20:242–244
    [Google Scholar]
  18. Gilboa-Garber N. 1972b; Purification and properties of hemagglutinin from Pseudomonas aeruginosaand its reaction with human blood cells. Biochimica et biophysica acta 273:165–173
    [Google Scholar]
  19. Gilboa-Garber N. 1982; Pseudomonas aeruginosalectins. In Methods in Enzymology, Complex Carbohydrates Part D 83 pp. 378–385 Edited by Ginsburg V. New York: Academic Press;
    [Google Scholar]
  20. Gilboa-Garber N. 1983; The biological functions of Pseudomonas aeruginosa lectins. In Lectins: Biology, Biochemistry, Clinical Biochemistry 3 pp. 495–502 Edited by Bog-Hansen T. C., Spengler G. A. Berlin & New York: Walter de Gruyter;
    [Google Scholar]
  21. Gilboa-Garber N., Mizrahi L. 1979; Interaction of the mannose-philic lectins of Pseudomonas aeruginosa with luminous species of marine enterobacteria. Microbios 26:31–36
    [Google Scholar]
  22. Gilboa-Garber N., Mizrahi L., Garber N. 1977; Mannose-binding hemagglutinins in extracts of Pseudomonas aeruginosa. Canadian Journal of Biochemistry 55:975–981
    [Google Scholar]
  23. Glick J., Malik Z., Garber N. 1981; Lectinbearing protoplasts of Pseudomonas aeruginosa induce capping in human peripheral blood lymphocytes. Microbios 32:181–188
    [Google Scholar]
  24. Hart D.A. 1980; Lectins in biological systems: applications to microbiology. American Journal of Clinical Nutrition 33:2416–2425
    [Google Scholar]
  25. Malamy M.H., Horecker B.H. 1964; Release of alkaline phosphatase from cells of E. coli upon lysozyme spheroplast formation. Biochemistry 3:1889–1893
    [Google Scholar]
  26. Nelson D.R., Cumsky M.G., Zusman D.R. 1981; Localization of myxobacterial hemagglutinin in the periplasmic space and on the cell surface of Myxococcus xanthus during developmental aggregation. Journal of Biological Chemistry 256:12589–12595
    [Google Scholar]
  27. Neu H.C., Heppel L.H. 1965; The release of enzymes from Escherichia coli by osmotic shock and during the formation of spheroplasts. Journal of Biological Chemistry 240:3685–3692
    [Google Scholar]
  28. Richards R.L., Moss J., Alving C.R., Fishman P.H., Brady R.O. 1979; Choleragen (cholera toxin): a bacterial lectin. Proceedings of the National Academy of Sciences of the United States of America 76:1673–1676
    [Google Scholar]
  29. Salit I.E., Gotschlich E.C. 1977; Hemagglutination by purified type I Escherichia coli pili. Journal of Experimental Medicine 146:1169–1181
    [Google Scholar]
  30. Sharabi Y., Gilboa-Garber N. 1980; Interactions of Pseudomonas aeruginosa hemagglutinins with Euglena gracilis, Chlamydomonas reinhardii andTetrahymena pyriformis. Journal of Protozoology 27:80–83
    [Google Scholar]
  31. Springer W.R., Haywood P.L., Barondes S.H. 1980; Endogenous cell surface lectin in Dictyoste-lium: quantitation elution by sugar and elicitation by divalent immunoglobulin. Journal of Cell Biology 87:682–690
    [Google Scholar]
  32. Weiss R.L., Fraser D. 1973; Surface structure of intact cells and spheroplasts of Pseudomonas aeruginosa. Journal of Bacteriology 113:963–968
    [Google Scholar]
  33. Without B., Van Heerikhuizen H., De Leij L. 1976; How does lysozyme penetrate through the bacterial outer membrane. Biochimica et biophysica acta 443:534–544
    [Google Scholar]
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