1887

Abstract

Salmonella isolates belonging to different serogroups have been analysed with respect to physicochemical surface properties and interaction with human polymorphonuclear granulocytes (PMNs). Most (22/34) recent isolates of the different serotypes showed hydrophilic surface properties and little if any negative charge accompanied by resistance to phagocytosis by PMN similar to the old laboratory S strains 395MS and S99 (main group). However, all isolates belonging to the serogroups Cl (5 isolates), E4 (2), O43 (1), and one out of three E1 isolates (C1/E4 group) differed from the main group. In aqueous biphasic partition in dextran-polyethyleneglycol (PEG) systems the bacteria in the main group accumulated in the PEG-rich phase to 55–97%, those in the C1/E4 group to less than 10%, and R-mutants only to 1–2%.

The bacteria in the C1/E4 group displayed a negative surface charge and a susceptibility to phagocytosis by PMNs that were greater than those for strains in the main group but much lower than those shown by the R-mutants. Bacteria belonging to serogroup C1 also displayed a significant susceptibility to hydrophobic interaction. The results are discussed in relation to the pathogenicity of salmonella.

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1983-10-01
2024-04-24
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References

  1. Bailey N.T.J. 1981 Statistical Methods in Biology London: Hodder & Stoughton;
    [Google Scholar]
  2. Crosa J.F., Brenner D.J., Ewing W.H., Falkow S. 1973; Molecular relationships among the Salmonellae. Journal of Bacteriology 115:307–315
    [Google Scholar]
  3. Duguid J.P., Old D.C. 1980; Adhesive properties in Enterobacteriaceae. Bacterial Adherence Receptors and Recognition, series B, vol. 6 pp. 185–217 Edited by Beachey E.H. London: Chapman & Hall;
    [Google Scholar]
  4. Duguid J.P., Darekar M.R., Wheater D.W.F. 1976; Fimbriae and infectivity inSalmonella typhimurium. Journal of Medical Microbiology 9:459–473
    [Google Scholar]
  5. Edebo L., Normann B. 1970; Virulence and immunogenicity of mutant strains ofSalmonella typhimurium. Acta pathologica et microbiologica scan-dinavica 78B:75–84
    [Google Scholar]
  6. Edebo L., Kihlström E., Magnusson K.-E., Stendahl O. 1980; The hydrophobic effect and charge effects in the adhesion of enterobacteria to animal cell surfaces and the influence of antibodies of different immunoglobulin classes. In Cell Adhesion and Motility Third Symposium of the British Society for Cell Biology pp. 65–101 Edited by Curtis A. S. G., Pitts J.D. Cambridge: Cambridge University Press;
    [Google Scholar]
  7. Edebo L., Nilsson B.-O., Lindberg A.A., Sven-Ungsson B., Coombs R.R.A. 1981; Class of serum antibodies towardsSalmonella enteritidis in gastro-enteritis, as measured by mixed reverse passive antiglobulin haemmagglutination (MRPAH). Acta pathologica et microbiologica scan-dinavica 89B:341–346
    [Google Scholar]
  8. Hed J. 1977; The extinction of fluorescence by crystal violet and its use to differentiate between attached and ingested microorganisms in phagocytosis. FEMS Microbiology Letters 1:357–361
    [Google Scholar]
  9. Hormaeche C.E. 1979; Natural resistance toSalmonella typhimurium in different inbred mouse strains. Immunology 37:311–318
    [Google Scholar]
  10. Kauffmann F. 1930; Die Technik Typhus-Paraty-phusgruppe er Typenbestimmung in der Zentralblatt fur Bakteriologie. Parasitenkunde und Infektions-krankheiten (Abteilung I,Originate A) 119:152–160
    [Google Scholar]
  11. Kihlström E., Magnusson K.-E. 1980; Association with HeLa cells of LPS mutants ofSalmonella typhimurium andSalmonella minnesota in relation to their physicochemical surface properties. Cell Biophysics 2:177–189
    [Google Scholar]
  12. Liang-Takasaki C.-J., Mäkelä P.H., Leive L. 1982; Phagocytosis of bacteria by macrophages: Changing the carbohydrate of lipopolysaccharide alters interaction with complement and macrophages. Journal of Immunology 128:1229–1235
    [Google Scholar]
  13. Lindberg A.A. 1980; Bacterial virulence factors - with particular reference toSalmonella bacteria. Scandinavian Journal of Infectious Diseases supplement 24:86–92
    [Google Scholar]
  14. Lindberg A.A., Le Minor L. 1983; Serology ofSalmonella. In Methods in Microbiology in the Press
    [Google Scholar]
  15. Mäkelä P.H., Valtonen M.V., Valtonen V.V. 1973; Role of O-antigen (lipopolysaccharide) factors in the virulence ofSalmonella. Journal of Infectious Diseases supplement 128:81–85
    [Google Scholar]
  16. Nikaido H. 1980; Nonspecific transport through the outer membrane. In Bacterial Outer Membranes, Biogenesis and Functions pp. 361–409 Edited by Inouy M. New York: Wiley;
    [Google Scholar]
  17. öhman L., Normann B., Stendahl O. 1981; Physicochemical surface properties ofEscherichia coli strains isolated from different types of urinary tract infections. Infection and Immunity 32:951–955
    [Google Scholar]
  18. öhman L., Magnusson K.-E., Stendahl O. 1982; The mannose-specific lectin activity ofEscherichia coli type 1 fimbriae assayed by agglutination of glycolipid-containing liposomes, erythrocytes, and yeast cells and hydrophobic interaction chromatography. FEMS Microbiology Letters 14:149–153
    [Google Scholar]
  19. Osborn M.J., Wu H.C.P. 1980; Proteins of the outer membrane of gram-negative bacteria. Annual Review of Microbiology 34:369–422
    [Google Scholar]
  20. Plant J.E., Glynn A.A. 1982; Genetic control of resistance toSalmonella typhimurium infection in high and low antibody responder mice. Clinical and Experimental Immunology 50:283–290
    [Google Scholar]
  21. Ruitenberg E.J., Kampelmacher E.H., Van Noorle Jansen M. 1971; Effect of the method of administration on the invasive capacity ofSalmonella. Zentralblatt für Bakteriologie (Originate A) 219:336–347
    [Google Scholar]
  22. Smith H.W., Parsell Z. 1971; The effect on virulence of converting the O antigen ofSalmonella cholerae-suis from 627 to 6,7 by phage. Journal of General Microbiology 81:217–224
    [Google Scholar]
  23. Stendahl O., Edebo L. 1972; Phagocytosis of mutants ofSalmonella typhimurium by rabbit polymorphonuclear cells. Acta pathologica et microbiologica scandinavica 80B:481–488
    [Google Scholar]
  24. Stendahl O., Magnusson K.-E., Tagesson C., Cunningham R., Edebo L. 1973a; Characterization of mutants ofSalmonella typhimurium by counter-current distribution in an aqueous two-polymer phase system. Infection and Immunity 7:573–577
    [Google Scholar]
  25. Stendahl O., Tagesson C., Edebo L. 1973b; Partition ofSalmonella typhimurium in a two-polymer aqueous system in relation to liability to phagocytosis. Infection and Immunity 8:36–41
    [Google Scholar]
  26. Valtonen M.V. 1977; Role of phagocytosis in mouse virulence ofSalmonella typhimurium recombinants with O antigen 6,7 or 4,12. Infection and Immunity 18:574–582
    [Google Scholar]
  27. Valtonen M.V., Plosila M., Valtonen V.V., Mäkelä P.H. 1975; Effect of the quality of the lipopolysaccharide on mouse virulence ofSalmonella enteritidis. Infection and Immunity 12:828–832
    [Google Scholar]
  28. Valtonen M.V., Nurminen M., Johansson V., Mäkelä P.H. 1977; Mouse virulence ofSalmonella typhimurium mutants deficient in two major outer membrane proteins. Infection and Immunity 18:454–458
    [Google Scholar]
  29. Valtonen V.V. 1970; Mouse virulence ofSalmonella strains: the effect of different smooth-type O side chains. Journal of General Microbiology 64:255–268
    [Google Scholar]
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