1887

Abstract

Summary: Salmonella isolates belonging to different serogroups have been analysed with respect to physicochemical surface properties and interaction with human polymorphonuclear granulocytes (PMNs). Most (22/34) recent isolates of the different serotypes showed hydrophilic surface properties and little if any negative charge accompanied by resistance to phagocytosis by PMN similar to the old laboratory S strains 395MS and S99 (main group). However, all isolates belonging to the serogroups Cl (5 isolates), E4 (2), O43 (1), and one out of three E1 isolates (C1/E4 group) differed from the main group. In aqueous biphasic partition in dextran-polyethyleneglycol (PEG) systems the bacteria in the main group accumulated in the PEG-rich phase to 55-97%, those in the C1/E4 group to less than 10%, and R-mutants only to 1-2%.

The bacteria in the C1/E4 group displayed a negative surface charge and a susceptibility to phagocytosis by PMNs that were greater than those for strains in the main group but much lower than those shown by the R-mutants. Bacteria belonging to serogroup C1 also displayed a significant susceptibility to hydrophobic interaction. The results are discussed in relation to the pathogenicity of salmonella.

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-129-10-3075
1983-10-01
2019-09-21
Loading full text...

Full text loading...

http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-129-10-3075
Loading
This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error