1887

Abstract

When was grown in a sodium polypectate/yeast extract/salts medium adjusted with sorbitol to water activity () values of 0·990 and 0·980, extracellular pectate lyase (PL) production was repressed, whereas intracellular PL levels were not affected. Inorganic solutes (NaCl. LiCl, KCl, NaSO and a NaCl/KCl/NaSO mixture) at 0·990 aw strongly stimulated the intracellular levels of PL (6-to 17-fold greater than the control), whereas extracellular levels were only slightly increased. Intra-and extracellular PL levels were not affected by LiCl, NaCl and KCl at 0·995 . A NaCl/sorbitol mixture (0·990 ) completely inhibited extracellular but not intracellular PL production. Lowering of the aw of cultures during mid-exponential growth phase with NaCl (0·990 ) resulted within 30 min in a 70-fold increase in intracellular PL activity. When sorbitol was used in similar experiments, extracellular PL production was inhibited to a greater extent than intracellular levels.

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1983-10-01
2021-10-22
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References

  1. Chatterjee A.K., Buchanan G.E., Behrens M.K., Starr M.P. 1979; Synthesis and excretion of polygalacturonic acid transeliminase inErwinia, Yersinia andKlebsiella species. Canadian Journal of Microbiology 25:94–102
    [Google Scholar]
  2. Chatterjee A.K, Thurn K.K., Tyrell D.J. 1981; Regulation of pectolytic enzymes in soft rotErwinia. . In Proceedings of the Fifth International Conference on Plant Pathogenic Bacteria pp. 252–262 Cali, Colombia Edited by Lozano J.C.
    [Google Scholar]
  3. Chirife J., Ferro Fontan C., Scorza O.C. 1981; The intracellular water activity of bacteria in relation to the water activity of the growth medium. Journal of Applied Bacteriology 50:475–479
    [Google Scholar]
  4. Kanemasa Y., Katayama T., Hayashi H., Takatsu T., Tomochika K., Okabe A. 1976; The barrier role of cytoplasmic membrane in salt tolerance mechanism inStaphylococcus aureus. . Zentralblattfiir Bakteriologie, Parasitenkunde, Infektionskrankheiten und Hygiene (Abteilung 1)Supplement 5:189–201
    [Google Scholar]
  5. Kennedy E.P. 1982; Osmotic regulation and the biosynthesis of membrane-derived oligosaccharides inEscherichia coli. . Proceedings of the National Academy of Sciences of the United States of America 79:1092–1095
    [Google Scholar]
  6. Kuo S.C., Lampen J.O. 1971; Osmotic regulation of invertase formation and secretion by protoplasts ofSaccharomyces. . Journal of Bacteriology 106:183–191
    [Google Scholar]
  7. Laimins L.A., Rhoads D.B., Epstein W. 1981; Osmotic control of kdp operon expression inEscherichia coli. . Proceedings of the National Academy of Sciences of the United States of America 78:464–468
    [Google Scholar]
  8. Measures J.C. 1975; Role of amino acids in osmoregulation of non-halophilic bacteria. Nature, London 257:398–400
    [Google Scholar]
  9. Mildenhall J.P., Prior B.A., Brand J.M. 1981a; Relation of water activity to growth and extracellular pectate lyase production byErwinia chrysanthemi. . In Proceedings of the Fifth Intemationl Conference on Plant Pathogenic Bacteria pp. 235–245 Cali, Colombia Edited by Lozano J.A.
    [Google Scholar]
  10. Mildenhall J.P., Prior B.A., Trollope L.A. 1981; b)Water relations ofErwinia chrysanthemi:Growth and extracellular pectic acid lyase production. Journal of General Microbiology 127:27–34
    [Google Scholar]
  11. Prior B.A., Kenyon C.P. 1980; Water relations of glucose-catabolizing enzymes inPseudomonas fluorescens. . Journal of Applied Bacteriology 48:211–222
    [Google Scholar]
  12. Prior B.A., Casaleggio C., Van Vuuren H.J.J. 1977; Psychometric determination of water activity in the higha w range. Journal of Food Protection 40:537–539
    [Google Scholar]
  13. Robinson R.A., Stokes R.H. 1955 Electrolyte Solutions London: Butterworth Scientific Publications;
    [Google Scholar]
  14. Roller S.D., Anagnostopoulos G.D. 1982; Accumulation of carbohydrate byEscherichia coliB/r/1 during growth at low water activity. Journal of Applied Bacteriology 52:425–434
    [Google Scholar]
  15. Scott W.J. 1953; Water relations ofStaphylococcus aureus at 30 °C. Australian Journal of Biological Sciences, 6:549–564
    [Google Scholar]
  16. Troller J. 1972; Effect of water activity on enterotoxin A production and growth ofStaphylococcus aureus. . Applied Microbiology 24:440–443
    [Google Scholar]
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