Summary: Isoelectric focusing of extracts of carrot root and apple fruit parenchyma infected by each of three isolates of showed the presence of forms of endopolygalacturonase (PG) with isoelectric points (pI) of about 4.5 (a minor peak) and 8.3-8.8. One isolate gave the hitherto unreported peak of pI 8.8 in both tissues, whereas the other two isolates gave a peak of 8.3. The molecular weights of the PGs of pI 8.3-8.8 were estimated to be about 30000. The variation in pi probably reflects the variability of No evidence of specific enzymic adaptation to host tissues was shown by the ability of PGs to cause changes in permeability of carrot and apple parenchyma.


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