The relationship between culture density or phase of growth at 24.5 °C and the ability of Candida albicans to form germ tubes when shifted to 37°C was investigated. Evidence is presented demonstrating germ tube production from liquid synthetic medium cultures at all phases of growth. Previous studies reported that only cells from stationary phase cultures were competent to form germ tubes. Comparisons between exponential and stationary phase cultures indicate more rapid and more synchronous germ tube production from cells growing in the exponential phase.
BedellG.W.,
SollD.R.1979; Effects of low concentrations of zinc on the growth and dimorphism of Candida albicans’, evidence for zinc-resistant and -sensitive pathways for mycelium formation. Infection and Immunity 26:348–354
BellW.M.,
ChaffinW.L.1980; Nutrient-limited yeast growth in Candida albicans: effect on yeast-mycelial transition. Canadian Journal of Microbiology 26:102–105
LeeK.L.,
BuckleyH.R.,
CampbellC.C.1975; An amino acid liquid synthetic medium for development of mycelial and yeast forms of Candida albicans. Sabouraudia 13:148–153
MitchellL.H.,
SollD.R.1979; Commitment to germ tube or bud formation during release from stationary phase in Candida albicans. Experimental Cell Research 120:167–179
SollD.R.,
BedellG.W.1978; Bud formation and the inducibility of pseudo-mycelium outgrowth during release from stationary phase in Candida albicans. Journal of General Microbiology 108:173–180