1887

Abstract

In yeast, thiopyronine (TP) acts both as a growth inhibitor (in the dark) and a photodynamic sensitizer (in the light). We have isolated a large number of TP-resistant mutants of and characterized them by genetic and photobiological techniques. Resistance to the growth-inhibitory (dark) effects of TP can arise by mutations in at least four separate genes. Growth-inhibitory and photodynamic effects of TP can be genetically separated, as evidenced by the finding that resistance mutants affected in three of the genes were not altered in their photodynamic response to the dye. In contrast, a mutation in the fourth gene simultaneously conferred high resistance to both the growth-inhibitory and photosensitizing effects of TP, suggesting that a mutation had occurred at a step common to the expression of both effects. Preliminary data suggest that this mutation reduces the ability of the cells either to take up, or bind, TP.

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/content/journal/micro/10.1099/00221287-129-1-63
1983-01-01
2021-08-02
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References

  1. Dujon B. 1981; Mitochondrial genetics and functions. In The Molecular Biology of the Yeast Saccharomyces, Life Cycles and Inheritance pp. 505–635 Strathem J. N., Jones E. W., Broach J. R. Edited by New York: Cold Spring Harbor Laboratory;
    [Google Scholar]
  2. Farber E. M. 1980; Psoralen and ultraviolet A (PUVA) - a critique. Journal of the American Academy of Dermatology 2:342–344
    [Google Scholar]
  3. Lochmann E.-R., Herrmann C., Pietsch I., Micheler A. 1976; Distribution of [3H]thiopyronine in yeast cells (Saccharomyces cerevisiae). Zeitschrift für Naturforschung 31C:481–483
    [Google Scholar]
  4. Lochmann E. -R., Käufer N., Schütze S., Kreutzfeldt C. 1981; Thiopyronine-sensitized photodynamic effect on RNA synthesis in Saccharomyces cells in vivo. International Journal of Radiation Biology 40:1–9
    [Google Scholar]
  5. Marquardt H., Von Laer U. 1966; Nichtmutagenität von Thiopyronin in einem Vorwärts- und Rückwartsmutationssystem der Hefe. Die Nawissenschaften 53:185
    [Google Scholar]
  6. Micheler A. 1972 Untersuchungen zum molekularen Mechanismus des photodynamischen Effekts Ph.D. thesis Freie Universitat Berlin, F.R.G:
    [Google Scholar]
  7. Micheler A., Lochmann E. -R. 1971; Die Aufnahme von Thiopyronin in Saccharomyceszellen XI. Mitteilung fiber photodynamische Wirkung von Farbstoffen. Studio biophysica 26:207–214
    [Google Scholar]
  8. Nishiyama-Watanabe S. 1976; Photodynamic action of thiopyronine on the respiration and fermenta-tion in yeast. International Journal of Radiation Biology 30:501–509
    [Google Scholar]
  9. Parrish J. A., Fitzpatrick T. B., Tannenbaum L., Pathak M. A. 1974; Photochemotherapy of psoriasis with oral methoxsalen and longwave ultra-violet light. New England Journal of Medicine 291:1207–1211
    [Google Scholar]
  10. Roth R. M., Dampier C. 1972; Dependence of ribonucleic acid synthesis on continuous protein synthesis in yeast. Journal of Bacteriology 109:773–779
    [Google Scholar]
  11. Roth R. M., Fogel S. 1971; A system selective for yeast mutants deficient in meiotic recombination. Molecular and General Genetics 112:295–305
    [Google Scholar]
  12. Roth R. M., Papierniak K. J., Anderson J. M. 1978; Mutations in Saccharomyces cerevisiae affecting sensitivity to photodynamic inactivation. Photochemistry and Photobiology 27:795–798
    [Google Scholar]
  13. Sherman F., Lawrence C. W. 1974 Saccharomyces.In Handbook of Genetics 1 pp. 359–393 King R. C. Edited by New York: Plenum Press;
    [Google Scholar]
  14. Spikes J. D. 1977; Photosensitization. In The Science of Photobiology pp. 87–112 Smith K. C. Edited by New York: Plenum Press;
    [Google Scholar]
  15. Stern R. S., Thibodeau L. A., Kleinerman R. A., Parrish J. A., Fitzpatrick T. B. 22 Participating Investigators 1979; Risk of cutaneous carcinoma in patients treated with oral methoxsalen photochemotherapy for psoriasis. New England Journal of Medicine 300:809–813
    [Google Scholar]
  16. Wolff K., Fitzpatrick T. B., Parrish J. A., Gschnait F., Gilchrest B., Hönigsmann H., Pathak M. A., Tannenbaum L. 1976; Photo-chemotherapy for psoriasis with orally administered methoxsalen. Archives of Dermatology 112:943–950
    [Google Scholar]
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