Mass Spectrometric Analysis of Drug-induced Changes in Na and K Contents of Single Bacterial Cells Free

Abstract

Time-dependent changes in the intracellular Na/K ratio of , induced by the nitrofuran derivative HN32 [2,4-diamino-6-(5-nitrofuryl-2)-5-ethylpyrimidine], were measured by laser-induced mass spectrometry of single bacterial cells. The results show good agreement with data on viable cell and total cell counts, release of ATP and CO production demonstrating that the single cell analysis of intracellular sodium and potassium concentrations may supply reliable information on cell viability and, furthermore, offer additional information not available from established gross methods.

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/content/journal/micro/10.1099/00221287-129-1-51
1983-01-01
2024-03-28
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References

  1. Anton A. H. 1960; The relation between the binding of sulfonamides to albumin and their antibacterial efficacy. Journal of Pharmacology and Experimental Therapy 129:282–292
    [Google Scholar]
  2. Glynn J. M., Karlish S. J. D., Cavieres J. D., Ellory J. C., Lew V. L., Jörgensen P. L. 1974; The effects of antiserum to (Na+, K+)-ATPase on the ion-transporting and hydrolytic activities of the enzyme. Annals of the New York Academy of Sciences 242:357–371
    [Google Scholar]
  3. Heinen H. J., Hillenkamp F., Kaufmann R., Schröder W., Wechsung R. 1980; LAMMA: a new laser microprobe mass analyzer for biomedicine and material analysis. In Recent Developments in Mass Spectrometry in Biochemistry and Medicine 6 pp. 435–451 Frigerio A., McCamish M. Edited by Amsterdam: Elsevier;
    [Google Scholar]
  4. Lopez J. M., Fortnagel P. 1981; Nitrofurantoin prompts the stringent response in Bacillus subtilis. Journal of General Microbiology 126:491–496
    [Google Scholar]
  5. Lundin A. 1981; Applications of firefly luminescence. In Proceedings of the 2nd International Symposium on Analytical Application of Bioluminescence and Chemiluminescence 1980 pp. 187–196 De Luca M. A., McElroy W. D. Edited by New York: Academic Press;
    [Google Scholar]
  6. Mcelroy W. D., De Luca M. A. 1981; The chemistry and applications of firefly luminescence. In Proceedings of the 2nd International Symposium on Analytical Applications of Bioluminescence and Chemiluminescence 1980 pp. 179–186 De Luca M. A., McElroy W. D. Edited by New York: Academic Press;
    [Google Scholar]
  7. Seydel J. K., Wempe E. 1980; A simple, fast and inexpensive kinetic method to differentiate between total and viable bacteria using coulter counter technique. Arzneimittel-Forschung/Drug Research 30:298–301
    [Google Scholar]
  8. Seydel U., Heinen H. J. 1980; First results on fingerprinting of single mycobacterial cells with LAMMA. In Recent Developments in Mass Spectrometry in Biochemistry and Medicine 6 pp. 489–496 Frigerio A., McCamish M. Edited by Amsterdam: Elsevier;
    [Google Scholar]
  9. Seydel U., Lindner B. 1981; Qualitative and quantitative investigations on mycobacteria with LAMMA. Fresenius’ Zeitschrift für analytische Chemie 308:253–257
    [Google Scholar]
  10. Seydel U., Lindner B., Seydel J. K., Brandenburg K. 1982; Detection of externally induced impairments in single bacterial cells by laser microprobe mass analysis. International Journal of Leprosy 50:90–95
    [Google Scholar]
  11. Shephard C. C. 1960; The experimental disease that follows the injection of human leprosy bacilli into foot pads of mice. Journal of Experimental Medicine 112:445–456
    [Google Scholar]
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