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Inoculation of wheat straw, contained in glass columns and moistened by continuous recirculation of a solution containing mineral salts, with a cellulolytic fungus, Penicillium corylophilum, and a N2-fixing anaerobe, Clostridium butyricum, increased the decomposition rate constant from 0·0096 d−1 to 0·0139 d−1 compared with non-inoculated straw. N2 fixation during the utilization of the straw resulted in again of 11.5 mg N (g straw lost)−1 over a period of 8 weeks at 25°C.
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