1887

Abstract

Addition of the antifungal antibiotic papulacandin B to an exponential culture of inhibited incorporation of glucose into the alkali-insoluble and alkali-soluble glucan fractions of the hyphal wall, although the rate of growth was practically unaltered. Synthesis of other cell wall components (i.e. galactomannan and chitin) was not affected. Papulacandin B also induced the proliferation of branches along the hyphae which continued to branch dichotomously resulting in a ‘colonial’ pattern of growth. Aculeacin A, another antifungal antibiotic that inhibited -glucan synthesis also caused morphological alterations similar to those described for papulacandin B. Inhibition of -glucan synthesis and the altered growth pattern persisted for several hours after removal of the antibiotic. Recovery of -glucan synthesis and restoration of the normal pattern of growth occurred simultaneously. Growth of in -sorbose medium also led to inhibition of -glucan synthesis and dichotomous branching.

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/content/journal/micro/10.1099/00221287-129-1-245
1983-01-01
2024-04-25
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References

  1. Algranati I. D., Behrens N., Carminatti H., Cabib E. 1966; Mannan synthetase from yeast. Methods in Enzymology 8:411–416
    [Google Scholar]
  2. Baguley B. C., Rommele G., Gruner J., Wehrli W. 1979; Papulacandin B: an inhibitor of glucan synthesis in yeast sphaeroplasts. European Journal of Biochemistry 97:345–351
    [Google Scholar]
  3. De Terra N., Tatum E. L. 1961; Colonial growth of Neurospora. Science 134:1066–1068
    [Google Scholar]
  4. Durán A., Cabib E. 1978; Solubilization and partial purification of yeast chitin synthase. Confirmation of the zymogenic nature of the enzyme. Journal of Biological Chemistry 253:4419–4425
    [Google Scholar]
  5. Fiddy C., Trinci A. P. J. 1976; Nuclei, septation, branching and growth of Geotrichum candidum. Journal of General Microbiology 97:185–192
    [Google Scholar]
  6. Keller F. A., Cabib E. 1971; Chitin and yeast budding.Properties jof chitin synthetase from Saccharomyces carlsbergensis. Journal of Biological Chemistry 246:160–166
    [Google Scholar]
  7. Mishra N. C., Tatum E. L. 1972; Effect of l-sorbose on polysaccharide synthetase of Neurospora crassa. Proceedings of the National Academy of Sciences of the United States of America 69:313–317
    [Google Scholar]
  8. Miyata M., Kitamura J., Miyata H. 1980; Lysis of growing fissing-yeast cells induced by Aculeacin A, a new antifungal antibiotic. Archives of Microbiology 127:11–16
    [Google Scholar]
  9. Mizoguchi J., Saito K., Mizuno K., Hayano K. 1977; On the mode of action of a new antifungal antibiotic, Aculeacin A: inhibition of cell wall synthesis in Saccharomyces cerevisiae. Journal of Antibiotics 30:308–313
    [Google Scholar]
  10. Mizuno K., Yagi S., Satoi S., Takada M., Hayashi M., Asano K., Matsuda T. 1977; Studies on Aculeacin: isolation and characterization of Aculeacin A. Journal of Antibiotics 30:297–302
    [Google Scholar]
  11. Molano J., Durán A., Cabib E. 1977; A rapid and sensitive assay for chitinase using tritiated chitin. Analytical Biochemistry 83:648–656
    [Google Scholar]
  12. Pérez P., Varona R., García-Acha I., Durán A. 1981; Effect of papulacandin B and aculeacinA on β-(l,3)-glucan synthase from Geotrichumlactis. FEBS Letters 129:249–252
    [Google Scholar]
  13. Santos T., Villanueva J. R., Nombela C. 1978; Regulation of β-(l,3)-glucanase synthesis in Penicillium italicum. Journal of Bacteriology 133:542–548
    [Google Scholar]
  14. Shematek E. M., Braatz J. A., Cabib E. 1980; Biosynthesis of the yeast cell wall. Preparation and properties of β-(l,3)-glucansynthetase. Journal of Biological Chemistry 255:888–894
    [Google Scholar]
  15. Traxler P., Gruner J., Auden J. A. L. 1977; Papulacandins, a new family of antibiotics with antifungal activity: fermentation, isolation, chemical and biological characterization of papulacandins A, B, C, and E. Journal of Antibiotics 30:289–296
    [Google Scholar]
  16. Trinci A. P. J. 1979; The duplication cycle and branching in fungi. In Fungal Walls and Hyphal Growth pp. 319–358 Burnett J. H., Trinci A. P. J. Edited by Cambridge: Cambridge University Press;
    [Google Scholar]
  17. Wilson R. W. 1970; Influence of cellobiose on the colonial morphology and glycosidases of several fungi. Canadian Journal of Microbiology 16:629–634
    [Google Scholar]
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