1887

Abstract

A K99 variant of B41 was produced by growing the parent strain in the presence of antiserum to K12K99. Two mannose-resistant and eluting (MRE) haemagglutinins with molecular weights greater than 20 × 10 were extracted from the cell surface of the variant. One was an anionic antigen, partially purified by ammonium sulphate and isoelectric point precipitation, which adhered to calf intestinal brush borders; it was a protein composed of subunits with mol. wt 34000. Electron microscopy showed that this material did not have a regular fimbrial appearance, but contained some fine fibrillar structures. A second MRE haemagglutinin which was also partially purified by ammonium sulphate precipitation, had a definite fimbrial structure, being a protein composed of two subunits of mol. wt 49 500 and 48000. This antigen was probably responsible for the fimbrial appearance of the K99 variant, but it was antigenically distinct from the anionic adhesin and did not adhere to calf intestinal brush borders.

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-129-1-235
1983-01-01
2024-04-25
Loading full text...

Full text loading...

/deliver/fulltext/micro/129/1/mic-129-1-235.html?itemId=/content/journal/micro/10.1099/00221287-129-1-235&mimeType=html&fmt=ahah

References

  1. Ashwell G. 1957; Colorimetric analysis of sugars. Methods in Enzymology 3:73–105
    [Google Scholar]
  2. Awad-Masalmeh M., Moon H. W., Runnels P. L., Schneider R. A. 1982; Pilus production, haem-agglutination, and adhesion by porcine strains of enterotoxigenic Escherichia coli lacking K88, K99, and 987P antigens. Infection and Immunity 35:305–313
    [Google Scholar]
  3. Bradley D. E. 1965; Replica and shadowing techniques. In Techniques for Electron Microscopy, 2nd edn. pp. 96–153 Kay D. H. Edited by Oxford: Blackwell Scientific Publications;
    [Google Scholar]
  4. Burrows M. R., Sellwood R., Gibbons R. A. 1976; Haemagglutinating and adhesive properties associated with K99 antigen of bovine strains of Escherichia coli . Journal of General Microbiology 96:269–275
    [Google Scholar]
  5. Chanter N. 1982; Structural and functional differences of the anionic and cationic antigens in K99 extracts of Escherichia coli B41. Journal of General Microbiology 128:1585–1589
    [Google Scholar]
  6. De Pamphilis M. L., Adler J. 1971; Fine structure and isolation of the hook-basal body complex of flagella from Escherichia coli and Bacillus subtilis. Journal of Bacteriology 105:384–395
    [Google Scholar]
  7. Ehresmann B., Imbault P., Weil J. H. 1973; Spectrophotometric determination of protein concentration of cell extracts containing tRNAs and rRNAs. Analytical Biochemistry 54:454–463
    [Google Scholar]
  8. Eshdat Y., Silverblatt F. J., Sharon N. 1981; Dissociation and reassembly of Escherichia coli type 1 pili. Journal of Bacteriology 148:308–314
    [Google Scholar]
  9. Fuchs S., Sela M. 1978; Immunoadsorbents. In Handbook of Experimental Immunology,, 3rd edn. pp. 10·1–10·6 Weir D. M. Edited by Oxford: Blackwell Scientific Publications;
    [Google Scholar]
  10. Hofstra H., Dankert J. 1979; Antigenic cross reactivity of major outer membrane proteins in Enterobacteriaceae species. Journal of General Microbiology 111:293–302
    [Google Scholar]
  11. Hunter W. M. 1978; Radioimmunoassay. In Handbook of Experimental Immunology,, 3rd edn. pp. 14·1–14·50 Weir D. M. Edited by Oxford: Blackwell Scientific Publications;
    [Google Scholar]
  12. Iino T. 1969; Genetics and chemistry of bacterial flagella. Bacteriological Reviews 33:454–475
    [Google Scholar]
  13. Isaacson R. E. 1977; K99 surface antigen of Escherichia coli: purification and partial characterisation. Infection and Immunity 15:272–279
    [Google Scholar]
  14. Isaacson R. E. 1981; Pili of enterotoxigenic Escherichia coli. In Proceedings of the Third International Symposium on Neonatal Diarrhoea pp. 213–236 Acres S. D., Forman A. J., Fast H. Edited by Saskatoon, Canada: VIDO;
    [Google Scholar]
  15. Laemmli U. K. 1970; Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature; London: 227681–685
    [Google Scholar]
  16. Lugtenberg B., Peters R., Bernheimer H., Berendsen W. 1976; Influence of cultural conditions and mutations on the composition of the outer membrane proteins of Escherichia coli. Molecular and General Genetics 147:251–262
    [Google Scholar]
  17. Martin R. B. 1964; Protein conformation. In Introduction to Biophysical Chemistry pp. 225–239 Martin R. B. Edited by New York: McGraw-Hill;
    [Google Scholar]
  18. Moon H. W., Nagy B., Isaacson R. E., Ørskov I. 1977; Occurrence of K99 antigen on Escherichia coli isolated from pigs and colonisation of pig ileum by K99+ enterotoxigenic E. coli from calves and pigs. Infection and Immunity 15:614–685
    [Google Scholar]
  19. Morris J. A., Hussaini S. N. 1974; Characterisation of the antibodies detected by the microscopic agglutination test for bovine leptospirosis. Journal of Hygiene 73:425–432
    [Google Scholar]
  20. Morris J. A., Thorns C. J., Sojka W. J. 1980; Evidence for two adhesive antigens on the K99 reference strain Escherichia coli B41. Journal of General Microbiology 118:107–113
    [Google Scholar]
  21. Ouchterlony O., Nilsson L.-A. 1978; Immunodiffusion and immunoelectrophoresis. In Handbook of Experimental Immunology,, 3rd edn. pp. 19·1–19·43 Weir D. M. Edited by Oxford: Blackwell Scientific Publications;
    [Google Scholar]
  22. Paakkanen J., Gotschlich E. C. 1979; Protein K: a new major outer membrane protein found in encapsulated Escherichia coli. Journal of Bacteriology 139:835–841
    [Google Scholar]
  23. Smith H., Williams, Lingood M. A. 1972; Further observations on Escherichia coli enterotoxins with particular regard to those produced by atypical piglet strains and by calf and lamb strains: the transmissible nature of these enterotoxins and of a K antigen possessed by calf and lamb strains. Journal of Medical Microbiology 5:243–250
    [Google Scholar]
  24. Sojka W. J. 1965; Escherichia coli in domestic animals and poultry. Review Series, Commonwealth. Bureau of Animal Health7
    [Google Scholar]
  25. Stirm S., Ørskov F., Ørskov I., Mansa B. 1967; Episome carried surface antigen K88 of Escherichia coli. II. Isolation and chemical analysis. Journal of Bacteriology 93:731–739
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-129-1-235
Loading
/content/journal/micro/10.1099/00221287-129-1-235
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error