1887

Abstract

Ecological factors that control the establishment of cellulolytic bacteria and ciliate protozoa in the lamb rumen were studied in meroxenic animals. Axenic lambs received dilutions of rumen liquor from either conventional lambs and sheep (pool A) or meroxenic lambs (pool B).

The total number of bacteria established in the rumen was between 10 and 5 × 10 g. In lambs inoculated with dilutions (10, 10, 10) of pool A, cellulolytic bacteria did not become established. However, subsequent inoculation with , resulted in colonization in lambs that had received 10 and 10 dilutions of pool A. However, became established in only one of three lambs that received the 10 dilution. Similar results were obtained for the protozoan sp.

With pool B, lambs were inoculated earlier and cellulolytic bacteria were established directly from the 10 and 10 inocula. establishment occurred readily when inoculated into the lambs which had received the 10 dilution of pool B.

Results obtained in this study suggest that establishment of cellulolytic bacteria and protozoa requires an abundant and complex flora and is favoured by early animal inoculation.

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-129-1-213
1983-01-01
2021-08-04
Loading full text...

Full text loading...

/deliver/fulltext/micro/129/1/mic-129-1-213.html?itemId=/content/journal/micro/10.1099/00221287-129-1-213&mimeType=html&fmt=ahah

References

  1. Allison M. J. 1965; Nutrition of rumen bacteria. In Physiology of Digestion in the Ruminant pp. 369–378 Dougherty R. W. Edited by London & Washington: Butterworths;
    [Google Scholar]
  2. Allison M. J., Robinson J. A., Booth G. D. 1979; Characterisation of bacterial population from the pig gut based upon the energy sources used for growth. Applied and Environmental Microbiology 37:1142–1151
    [Google Scholar]
  3. Barr M. E. J., Mann S. O., Richardson A. J., Stewart C. S., Wallace R. S. 1980; Establish-ment of ureolytic staphylococci in the rumen of gnotobiotic lambs. Journal of Applied Bacteriology 49:325–330
    [Google Scholar]
  4. Bonhomme A., Fonty G., Senaud J. 1982; Essai d’obtention et de survie de ciliés entodiniomorphes du rumen en cultures axéniques. Annales de Microbiologie de l’Institut Pasteur 133B:335–341
    [Google Scholar]
  5. Bryant M. P. 1973; Nutritional requirements of the predominant rumen cellulolytic bacteria. Federation Proceedings 32:1809–1813
    [Google Scholar]
  6. Bryant M. P. 1977; Microbiology of the rumen. In Dukes’ Physiology of Domestic Animals, 9th edn. pp. 287–304 Swenson M. J. Edited by Cornell University Press: Ithaca & London;
    [Google Scholar]
  7. Bryant M. P., Burkey L. A. 1953; Cultural methods and some characteristics of some of the more numerous groups of bacteria in the bovine rumen. Journal of Dairy Science 36:205–217
    [Google Scholar]
  8. Cheng K. J., Wallace J. J. 1979; The mechanism of passage of endogenous urea through the rumen wall and the role of ureolytic epithelium in the urea flux. British Journal of Nutrition 42:553–557
    [Google Scholar]
  9. Church D. C. 1970; Rumen microbiology. In Digestive Physiology and Nutrition of Ruminants, 1st edn. 1 pp. 151–197 Corvallis, Oregon U.S.A: O.S.U. Book Stores;
    [Google Scholar]
  10. Clarke R. T. J. 1977; Protozoa in the rumen. In Microbial Ecology of the Gut pp. 251–275 Clarke R. T. J., Bauchop T. Edited by New York, London, San Francisco: Academic Press;
    [Google Scholar]
  11. Coleman G. S. 1975; The interrelations between rumen ciliate protozoa and bacteria. In Digestion and Metabolism in the Ruminant pp. 149–164 McDonald I. W., Warner A. C. I. Edited by Armidale, New South Wales, Australia: University of New England Publishing Unit;
    [Google Scholar]
  12. Dehority B. A., Grubb S. A. 1976; Basal medium for the selective enumeration of rumen bacteria utilizing specific energy sources. Applied and Environmental Microbiology 32:703–710
    [Google Scholar]
  13. Dehority B. A., Scott H. W., Kowalerk P. 1967; Volatile fatty acids requirements of cellulolytic rumen bacteria. Journal of Bacteriology 94:537–543
    [Google Scholar]
  14. Eadie J. M., Hobson P. N. 1962; Effect of presence or absence of ciliates on the total bacteria count in lambs. Nature; London: 193503–505
    [Google Scholar]
  15. Eadie J. M., Mann S. O. 1970; Development of the rumen microbial populations: high starch diets and instability. In Physiology of Digestion and Metabolism in the Ruminant pp. 335–347 Phillipson A. T. Edited by Newcastle upon Tyne, England: Oriel University Press;
    [Google Scholar]
  16. Giesecke D. 1968; Zur Kemzahlbestimmung der anaeroben lactilytischen Bakteriengruppe im Pansen. Zentralblatt fur Bakteriologie (I. Abteilung) 109:101–106
    [Google Scholar]
  17. Gouet PH., Riou Y., Contrepois M., Dubourguier H. C. 1979; Efficacité d’une technique simple de production d’agneaux axéniques et de veaux oligox#x00E9;niques par décontamination à la naissance. Annales de recherches vétérinaires 10:49–51
    [Google Scholar]
  18. Grubb J. A., Dehority B. A. 1976; Variation in colony counts of total viable anaerobic rumen bacteria as influenced by media and cultural methods. Applied and Environmental Microbiology 31:262–267
    [Google Scholar]
  19. Henning P. A. 1979; Examination of methods for enumerating hemicellulose-utilizing bacteria in the rumen. Applied and Environmental Microbiology 38:13–17
    [Google Scholar]
  20. Hobson P. N. 1966; Rumen bacteria. Methods in Microbiology 3B:133–149
    [Google Scholar]
  21. Hobson P. N., Howard B. H. 1969; Microbial transformations. In Handbuch der Tierernährung 1 pp. 207–254 Lenkeit W., Brenem K., Craseman E. Edited by Hamburg & Berlin: Paul Parey;
    [Google Scholar]
  22. Hungate R. E. 1966a The Rumen and its Microbes New York & London : Academic Press;
    [Google Scholar]
  23. Hungate R. E. 1966b; A roll tube method for cultivation of strict anaerobes. Methods in Micro-biology 3B:117–132
    [Google Scholar]
  24. Jouany J. P. 1978 Contribution à l’étude des protozoaires ciliés du rumen, leur dynamique, leur rôle dans la digestion et leur intérêt pour le ruminant Thèse Doctorat d’Etat, no. d’ordre 256. Université de Clermont II. France:
    [Google Scholar]
  25. Kurihara Y., Eadie J. M., Hobson P. N., Mann S. O. 1968; Relationships between bacteria and ciliate protozoa in the sheep rumen. Journal of General Microbiology 51:267–288
    [Google Scholar]
  26. Lysons R. J., Alexander T. J. L. 1975; The gnotobiotic ruminant and in vivo studies of defined bacterial populations. In Digestion and Metabolism in the Ruminant pp. 180–192 McDonald I. W., Warner A. C. I. Edited by Armidale, New South Wales, Australia: University of New England Publishing Unit;
    [Google Scholar]
  27. Lysons R. J., Alexander T. J. L., Hobson P. N., Mann S. O., Stewart C. S. 1971; Establishment of a limited rumen microflora in gnotobiotic lambs. Research in Veterinary Science 12:486–487
    [Google Scholar]
  28. Lysons R. J., Alexander T. J. L., Wellstead P. D., Hobson P. N., Mann S. O., Stewart C. S. 1976a; Defined bacterial population in the rumen of gnotobiotic lambs. Journal of General Microbiology 94:257–269
    [Google Scholar]
  29. Lysons R. J., Alexander T. J. L., Wellstead P. D., Jennings I. W. 1976b; Observations on the alimentary tract of gnotobiotic lambs. Research in Veterinary Science 20:70–76
    [Google Scholar]
  30. Males J. 1973 Ration digestibility, rumen bacteria and several rumen parameters in sheep bom and reared in isolation Dissertation, The Ohio State University:
    [Google Scholar]
  31. Mann S. O. 1968; An improved method for determining cellulolytic activity in anaerobic bacteria. Journal of Applied Bacteriology 31:241–244
    [Google Scholar]
  32. Mann S. O., Stewart C. S. 1974; Establishment of a limited rumen flora in gnotobiotic lambs fed on a roughage diet. Journal of General Microbiology 84:379–382
    [Google Scholar]
  33. Petkov A., Enev E. I. 1979; Rumen digestion parameters in lambs fed with pelleted diet. Annales de recherches vétérinaires 10:440–441
    [Google Scholar]
  34. Peyraud J. L., Thivend P., Gouet PH. 1981; Role of digestive microflora on starch digestion in the preruminant lamb. In Recent Advances in Germfree Research, Proceedings of the VIIth International Symposium on Gnotobiology pp. 285–288 Sasaki S., Ozawa A., Hashimoto K. Edited by Tokyo: Tokai University Press;
    [Google Scholar]
  35. Prins R. A. 1977; Biochemical activities of gut microorganisms. In Microbial Ecology of the Gut pp. 73–183 Clarke R. T. J., Bauchop T. Edited by London, New York, San Francisco: Academic Press;
    [Google Scholar]
  36. Raibaud P., Dickinson A. B., Sacquet E., Charlier R., Mocquot G. 1966; La microflore du tube digestif du rat. I. Techniques d’étude et milieux de culture proposés. Annales de l’Institut Pasteur 110:568–590
    [Google Scholar]
  37. Riou Y., Gouet PH., Dubourguier H. C., Contrepois M., Dardillat C., Levaivre J. 1977; Techniques for obtaining fistulization and rearing of axenic or gnotoxenic lambs, kids and calves. Annales de recherches vétérinaires 8:13–24
    [Google Scholar]
  38. Senaud J., Groliere C. A., Zainab B., Grain J., Jouany J. P. 1980; Implantation et developement des protozoaires ciliés (Polyplastron multivesiculatum, Entodinium sp., Isotricha prostoma) dans le rumen de moutons recevant différents régimes alimentaires. I. Régime luzeme-orge. Protistologica 16:329–337
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-129-1-213
Loading
/content/journal/micro/10.1099/00221287-129-1-213
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error