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DNA colony hybridization was found to have a high generic and strain specificity for Rhizobium trifolii. We have assessed the use of this technique to determine occupancy of nodules by strains of Rhizobium and found that its high degree of specificity allowed successful identification of rhizobia from nodules. Other techniques currently available for determining the Rhizobium strain involved in forminsg a particular nodule lack specificity or interfere with symbiotic capabilities of the rhizobia.
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