1887

Abstract

DNA colony hybridization was found to have a high generic and strain specificity for We have assessed the use of this technique to determine occupancy of nodules by strains of and found that its high degree of specificity allowed successful identification of rhizobia from nodules. Other techniques currently available for determining the strain involved in forminsg a particular nodule lack specificity or interfere with symbiotic capabilities of the rhizobia.

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/content/journal/micro/10.1099/00221287-129-1-207
1983-01-01
2021-08-03
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References

  1. Beynon J. C., Josey D. P. 1980; Demonstration of heterogeneity in a natural population ofRhizobium phaseoli using variation in intrinsic antibiotic resistance. Journal of General Microbiology 118:437–442
    [Google Scholar]
  2. Bohlool B. B., Schmidt E. L. 1980; The immunofluorescence approach in microbial ecology. Microbial Ecology 4:203–241
    [Google Scholar]
  3. Date R. A., Brockwell J. 1978; Rhizobium strain competition and host interaction for nodulation. In Plant Relations in Pastures pp. 202–216 Wilson J. R. Edited by East Melbourne: CS1RO;
    [Google Scholar]
  4. Grunstein M., Hogness D. S. 1975; Colony hybridization: a method for the isolation of cloned DNAs that contain a specific gene. Proceedings of the National Academy of Sciences of the United States of America 72:3961–3965
    [Google Scholar]
  5. Lowendorf H. S. 1980; Factors affecting survival ofRhizobium in soil. Advances in Microbial Ecology 4:87–125
    [Google Scholar]
  6. Schwinghamer E. A., Dudman W. F. 1973; Evaluation of spectinomycin resistance as a marker for ecological studies withRhizobium spp. Journal of Applied Bacteriology 36:263–272
    [Google Scholar]
  7. Vincent J. M. 1970 A Manualfor the Practical Study of the Root-Nodule Bacteria Oxford: Blackwell;
    [Google Scholar]
  8. Wetmur J. G. 1976; Hybridization and renaturation kinetics of nucleic acids. Annual Review of Biophysics and Bioengineering 5:337–361
    [Google Scholar]
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