RT Journal Article SR Electronic(1) A1 Nicholson-Guthrie, Catherine S. A1 Hudock, George A.YR 1983 T1 Growth, Ultrastructure and Carotenoid Spectra of the Chlorophyll-less y-y Mutant of Chlamydomonas reinhardtii JF Microbiology, VO 129 IS 1 SP 159 OP 165 DO https://doi.org/10.1099/00221287-129-1-159 PB Microbiology Society, SN 1465-2080, AB The isolation of a stable, chlorophyll-less Mendelian mutant (y-y) of Chlamydomonas reinhardtii is described and its growth, carotenoid spectra and ultrastructure are compared to chlorophyll-containing strains. Unlike most chlorophyll-less mutants, y-y did not die in the light but grew as bright yellow colonies. It was unable to grow in a mineral medium with CO2 and it required acetate for growth. When compared to green C. reinhardtii strains, y-y grew slower in the light but at about the same rate in the dark. Light-grown and dark-grown y-y cells had no detectable chlorophyll and similar amounts of total carotenoids, which were 20–35% of the values for the green strains. The only noticeable difference in ultrastructure between y-y and the green cells was in the inner chloroplast membranes. In the light and dark y-y lacked lamellae, although the outer chloroplast membrane was retained., UL https://www.microbiologyresearch.org/content/journal/micro/10.1099/00221287-129-1-159