%0 Journal Article %A Nicholson-Guthrie, Catherine S. %A Hudock, George A. %T Growth, Ultrastructure and Carotenoid Spectra of the Chlorophyll-less y-y Mutant of Chlamydomonas reinhardtii %D 1983 %J Microbiology, %V 129 %N 1 %P 159-165 %@ 1465-2080 %R https://doi.org/10.1099/00221287-129-1-159 %I Microbiology Society, %X The isolation of a stable, chlorophyll-less Mendelian mutant (y-y) of Chlamydomonas reinhardtii is described and its growth, carotenoid spectra and ultrastructure are compared to chlorophyll-containing strains. Unlike most chlorophyll-less mutants, y-y did not die in the light but grew as bright yellow colonies. It was unable to grow in a mineral medium with CO2 and it required acetate for growth. When compared to green C. reinhardtii strains, y-y grew slower in the light but at about the same rate in the dark. Light-grown and dark-grown y-y cells had no detectable chlorophyll and similar amounts of total carotenoids, which were 20–35% of the values for the green strains. The only noticeable difference in ultrastructure between y-y and the green cells was in the inner chloroplast membranes. In the light and dark y-y lacked lamellae, although the outer chloroplast membrane was retained. %U https://www.microbiologyresearch.org/content/journal/micro/10.1099/00221287-129-1-159