1887

Abstract

sp. MT14, isolated from soil as a result of its ability to utilise -toluate as sole source of carbon, contains two large catabolic plasmids. One of these, pWW14, is a TOL plasmid of about 270 kb which determines the ability to grow on toluene, -xylene and -xylene as sole carbon sources. The other, pWW17, which is about 280 kb, determines both the ability to grow on phenylacetate and resistance to mercury salts. Growth of MT14 on benzoate results in the segregation of derivatives in which either (i) one or both plasmids are completely lost, or (ii) one or both plasmids have undergone a large deletion which results in a change in the phenotype determined by that plasmid. Deletion of about 100 kb from pWW14 results in a regulatory mutation which allows its host to grow on -xylene but not on its metabolite -toluate (B3 mutants). Deletion of about 95 kb from pWW17 results in loss of the ability of its host to grow on phenylacetate but the mercury resistance is not lost.

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/content/journal/micro/10.1099/00221287-129-1-153
1983-01-01
2024-12-05
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References

  1. Dagley S., Fewster M. E., Happold F. C. 1953; The bacterial oxidation of aromatic compounds. Journal of General Microbiology 8:1–7
    [Google Scholar]
  2. Kunita N. 1955; Bacterial oxidation of phenylacetic acid. I. The pathway through homoprotocatechuic acid. Medical Journal of Osaka University 6:697–702
    [Google Scholar]
  3. Pickup R. W., Williams P. A. 1982; Spontaneous deletions in the TOL plasmid pWW20 which give rise to the B3 regulatory mutants of Pseudomonas putida MT20. Journal of General Microbiology 128:1385–1390
    [Google Scholar]
  4. Wheatcroft R., Williams P. A. 1981; Rapid methods for the study of both stable and unstable plasmids in Pseudomonas. Journal of General Microbiology 124:433–437
    [Google Scholar]
  5. Williams P. A., Murray K. 1974; Metabolism of benzoate and the methylbenzoates by Pseudomonas putida (arvilla) mt-2: evidence for the existence of a TOL plasmid. Journal of Bacteriology 120:416–423
    [Google Scholar]
  6. Williams P. A., Worsey M. J. 1976; Ubiquity of plasmids in coding for toluene and xylene metabolism in soil bacteria: evidence for the existence of new TOL plasmids. Journal of Bacteriology 125:818–828
    [Google Scholar]
  7. Worsey M. J., Williams P. A. 1975; Metabolism of toluene and xylenes by Pseudomonas putida(arvilla) mt-2: evidence for a new function of the TOL plasmid. Journal of Bacteriology 124:7–13
    [Google Scholar]
  8. Worsey M. J., Williams P. A. 1977; Characterisation of a spontaneously occurring mutant of the TOL20 plasmid in Pseudomonas putidaMT20: possible regulatory implications. Journal of Bacteriology 130:1149–1158
    [Google Scholar]
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