Summary: A method is described for deletion mapping of essential genes in . It involves the isolation of secondary-site insertions of c 1857 plac 5 into an F’ plasmid. The transposed Z gene is useful both for the ready screening of plasmid–phage cointegrates and for rapid analysis of deletions that extend from the site of phage integration into the bacterial genes carried on the substituted plasmid. Such deletions may be used to ‘hook-up’ bacterial cistrons to the powerful promoter. We report the application of this technique to the study of the interval, a cluster of genes encoding components of the transcription–translation apparatus.


Article metrics loading...

Loading full text...

Full text loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error