1887

Abstract

A spore coat protein of QM B1551 was purified from an SDS-DTT extract of lysozyme-digested spores using Sephadex, DEAE-cellulose chromatography and polyacrylamide disc gel electrophoresis. The molecular weight of the protein was approximately 12000. It constituted about 13% of spore dry weight and was rich in lysine, aspartic acid, glycine and alanine residues. The sum of these four amino acids constituted 53.9% of the total amino acids. Small amounts of phosphorus and sugar were detected, but no amino sugar. The protein was synthesized in the cytosol of sporulating mother cells at , and accumulated on the forespores.

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/content/journal/micro/10.1099/00221287-128-9-2149
1982-09-01
2024-12-08
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