1887

Abstract

An extracellular soluble common protein (ECP) has been purified from extracellular soluble fractions of exponential phase cultures of OMZ9, of a representative strain of each of Bratthall's seven serological groups of , and of one strain each of and . The ECP antigens from the different strains were prepared from SDS-dissociated immunoprecipitates by affinity chromatography on an anti-rabbit immunoglobulin column. The identity of such purified ECP antigens was demonstrated by their behaviour in immunodiffusion analysis, in SDS–PAGE, in which an identical molecular weight (60000) was found, and by virtue of their similar amino acid and sugar compositions. This common antigen (ECP) consisted of 90% protein and 10% sugar.

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1982-09-01
2024-12-10
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References

  1. Archer D. B. 1979; Immunoprecipitation of Triton X-100-solubilized Mycoplasma mycoides proteins. Journal of General Microbiology 115:111–116
    [Google Scholar]
  2. Bjerrum O. J., Lundahl P. 1976; Detergent-con-taining gels for immunological studies of solubilized erythrocyte membranes components. In A Manual for Quantitative Immunoelectrophoresis pp. 139–143 Axelsen N. M., Krohll J., Weeke B. Edited by Oslo, Bergen, Tromse: Universitets Forlaget;
    [Google Scholar]
  3. Bratthall D. 1970; Demonstration of five serological groups of Streptococcus strains, resembling Streptococcus mutans. Odontologisk revy 21:143–152
    [Google Scholar]
  4. Brown T. A., Bleiweis A. S. 1979; Chemical, immunological and structural studies of the cross reactive antigens of Streptococcus mutans AHT and B12. Infection and Immunity 24:326–336
    [Google Scholar]
  5. Burgess T. E., Edwards J. R. 1973; Chemical characterization of a cell wall antigen from Streptococcus mutans FA-1. Infection and Immunity 8:491–493
    [Google Scholar]
  6. Carlsson J. 1972; Nutritional requirements of Streptococcus sanguis. Archives of Oral Biology 17:1327–1332
    [Google Scholar]
  7. Dankert J., Hofstra H. 1978; Antibodies against outer membrane proteins in rabbit antisera prepared against Escherichia colt O26 K60. Journal of General Microbiology 104:311–320
    [Google Scholar]
  8. Davis B. J. 1964; Disc electrophoresis. II. Method and application to human serum proteins. Annals of the New York Academy of Sciences 121:404–427
    [Google Scholar]
  9. Ey P. L., Prowse S. J., Jenkin C. R. 1978; Isolation of pure IgG,, IgG2 and IgG2b immunoglobulins from mouse serum using protein A-Sepharose. Immunochemistry 15:429–436
    [Google Scholar]
  10. Hamada S., Slade H. D. 1980; Biology, immunology and cariogenicity of Streptococcus mutans. Microbiological Reviews 44:331–384
    [Google Scholar]
  11. Hughes M., Marchardy S. M., Sheppard A. J., Woods N. C. 1980; Evidence for an immunological relationship between Streptococcus mutans and human cardiac tissue. Infection and Immunity 27:576–588
    [Google Scholar]
  12. Iacono V. J., Taubman M. A., Smith D. J., Levine M. J. 1975; Isolation and immunochemical charac-terization of the group-specific antigen of Streptococcus mutans 6715. Infection and Immunity 11:117–128
    [Google Scholar]
  13. Inouye S., Takeiski K., De Martini M., Hiroshima A., Inouye M. 1976; Lipoprotein from the outer membrane of Escherichia coli: purification, paracrystallization, and some properties of its free form. Journal of Bacteriology 127:555–563
    [Google Scholar]
  14. Kelstrup J., Funder-Nielsen I. D. 1974; Adhesion of dextran to Streptococcus mutans. Journal of General Microbiology 81:485–489
    [Google Scholar]
  15. Kuramitsu H. K., Ingersoll L. 1978; Interaction of glucosyltransferases with the cell surface of Streptococcus mutans. Infection and Immunity 20:652–659
    [Google Scholar]
  16. Lehner T., Russell M. W., Caldwell J. 1980; Immunization with a purified protein from Streptococcus mutans against dental caries in Rhesus monkeys. Lancet 1:995–996
    [Google Scholar]
  17. Mccabe M. M., Smith E. E. 1973; Origin of the cell associated dextransucrase of Streptococcus mutans. Infection and Immunity 7:829–838
    [Google Scholar]
  18. Morris D. L. 1948; Quantitative determination of carbohydrate with Dreywood’s anthrone reagent. Science 107:254–258
    [Google Scholar]
  19. Mukasa H., Slade H. D. 1973; Structure and immunological specificity of the Streptococcus mutans group b cell wall antigen. Infection and Immunity 7:578–585
    [Google Scholar]
  20. Nesbitt W. E., Staat R., Rosan B., Taylor K. G., Doyle R. J. 1980; Association of protein with the cell wall of Streptococcus mutans. Infection and Immunity 28:118–126
    [Google Scholar]
  21. Ouchterlony O. 1958; Diffusion in gel methods for immunological analysis. Progress in Allergy 5:1–19
    [Google Scholar]
  22. Owen P., Doherty H. 1979; Immunochemical analysis of Triton XI00 insoluble residues from Micrococcus lysodeikticus membranes. Journal of Bacteriology 140:881–887
    [Google Scholar]
  23. Perch B., Kjems E., Ravn T. 1974; Biochemical and serological properties of Streptococcus mutans from various human and animal sources. Acta pathologica et microbiologica scandinavica section B 82:357–370
    [Google Scholar]
  24. Russell M. W., Lehner T. 1978; Characterization of antigens extracted from cells and culture fluids of Streptococcus mutans serotype c. Archives of Oral Biology 23:7–15
    [Google Scholar]
  25. Russell M. W., Bergmeier L. A., Zanders E. D., Lehner T. 1980a; Protein antigens of Streptococcus mutans. Purification and properties of a double antigen and its protease-resistant component. Infection and Immunity 28:486–493
    [Google Scholar]
  26. Russell M. W., Challacombe S. J., Lehner T. 1980b; Specificity of antibodies induced by Streptococcus mutans during immunization against dental caries. Immunology 40:97–106
    [Google Scholar]
  27. Russell R. R. B. 1979; Wall-associated protein antigens of Streptococcus mutans. Journal of General Microbiology 114:109–115
    [Google Scholar]
  28. Schöller M., Klein J. P., Frank R. M. 1981; Common antigens of streptococcal and non streptococcal oral bacteria: immunochemical studies of extracellular and cell-wall associated antigens from Streptococcus sanguis, Streptococcus mutans, Lactobacillus salivarius and Actinomyces viscosus. Infection and Immunity 31:52–60
    [Google Scholar]
  29. Stone J. S., Patterson J. M., Phillips P. 1979; Isolation and purification of bovine IgM by dissociating immunoglobulin-Brucella complexes. Journal of Immunological Methods 31:379–387
    [Google Scholar]
  30. Weber K., Osborn M. 1969; The reliability of molecular weight determination by dodecyl sulphate polyacrylamide gel electrophoresis. Journal of Biological Chemistry 244:4406–4412
    [Google Scholar]
  31. Wetherell J. R., Bleiweis A. S. 1978; Antigens of Streptococcus mutans. Isolation of a serotype-specific and a cross-reactive antigen from walls of strain V.100 (serotype e). Infection and Immunity 19:160–169
    [Google Scholar]
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