RT Journal Article SR Electronic(1) A1 Lambden, Paul R.YR 1982 T1 Biochemical Comparison of Pili from Variants of Neisseria gonorrhoeae P9 JF Microbiology, VO 128 IS 9 SP 2105 OP 2111 DO https://doi.org/10.1099/00221287-128-9-2105 PB Microbiology Society, SN 1465-2080, AB Four different types of pili produced by variants of Neisseria gonorrhoeae P9 were isolated and characterized. The pili differed in subunit molecular weight with SDS-PAGE and in subunit isoelectric point on agarose gels. Isoelectric points of the major molecular species in Tritonagarose gels of octylglucoside solubilized pili were: δ, pI 6·5; α;, pI 6·0; β, pI 5·3 and γ, pI 5·5. Amino acid analyses of pili showed close homology between different types but a reduction in the content of aspartate and serine was notable in the low molecular weight δ pili; also β and γ pili contained more alanine residues. Structural homology was also demonstrated in peptide maps of tryptic/chymotryptic digests of pili with several major peptides apparently common to all four pilus types., UL https://www.microbiologyresearch.org/content/journal/micro/10.1099/00221287-128-9-2105