@article{mbs:/content/journal/micro/10.1099/00221287-128-9-2105, author = "Lambden, Paul R.", title = "Biochemical Comparison of Pili from Variants of Neisseria gonorrhoeae P9", journal= "Microbiology", year = "1982", volume = "128", number = "9", pages = "2105-2111", doi = "https://doi.org/10.1099/00221287-128-9-2105", url = "https://www.microbiologyresearch.org/content/journal/micro/10.1099/00221287-128-9-2105", publisher = "Microbiology Society", issn = "1465-2080", type = "Journal Article", abstract = "Four different types of pili produced by variants of Neisseria gonorrhoeae P9 were isolated and characterized. The pili differed in subunit molecular weight with SDS-PAGE and in subunit isoelectric point on agarose gels. Isoelectric points of the major molecular species in Tritonagarose gels of octylglucoside solubilized pili were: δ, pI 6·5; α;, pI 6·0; β, pI 5·3 and γ, pI 5·5. Amino acid analyses of pili showed close homology between different types but a reduction in the content of aspartate and serine was notable in the low molecular weight δ pili; also β and γ pili contained more alanine residues. Structural homology was also demonstrated in peptide maps of tryptic/chymotryptic digests of pili with several major peptides apparently common to all four pilus types.", }