1887

Abstract

Four different types of pili produced by variants of P9 were isolated and characterized. The pili differed in subunit molecular weight with SDS-PAGE and in subunit isoelectric point on agarose gels. Isoelectric points of the major molecular species in Tritonagarose gels of octylglucoside solubilized pili were: , pI 6·5; α;, pI 6·0; , pI 5·3 and γ, pI 5·5. Amino acid analyses of pili showed close homology between different types but a reduction in the content of aspartate and serine was notable in the low molecular weight pili; also and γ pili contained more alanine residues. Structural homology was also demonstrated in peptide maps of tryptic/chymotryptic digests of pili with several major peptides apparently common to all four pilus types.

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-128-9-2105
1982-09-01
2024-04-19
Loading full text...

Full text loading...

/deliver/fulltext/micro/128/9/mic-128-9-2105.html?itemId=/content/journal/micro/10.1099/00221287-128-9-2105&mimeType=html&fmt=ahah

References

  1. Bates D. L., Perham R. N., Coggins J. R. 1975; Methods for obtaining peptide maps of proteins on a subnanomole scale. Analytical Biochemistry 68:175–184
    [Google Scholar]
  2. Brinton C. C., Bryan J., Dillon J. -A., Guerina N., Jacobson L. J., Labik A., Lee S., Levine A., Lim S., Mcmichael J., Polen S., Rogers K., To A. C. -C., To S. C. -M. 1978; Uses of pili in gonorrhea control: role of bacterial pili in disease, purification and properties of gonococcalpili, and progress in the development of a gonococcalpilus vaccine for gonorrhea. In Immunobiology of Neisseria gonorrhoeae pp. 145–154 Brooks G. F., Gotschlich E. C., Holmes K. K., Sawyer W. D., Young F. E. Edited by Washington, D.C: American Society for Microbiology;
    [Google Scholar]
  3. Buchanan T. M. 1977; Surface antigens: pili. In The Gonococcus pp. 255–272 Roberts R. B. Edited by New York: John Wiley;
    [Google Scholar]
  4. Buchanan T. M., Pearce W. A., Chen K. C. S. 1978; Attachment of Neisseria gonorrhoeae pili to human cells, and investigations of the chemical nature of the receptor for gonococcalpili. In Immunobiology of Neisseria gonorrhoeae pp. 242–249 Brooks G. F., Gotschlich E. C., Holmes K. K., Sawyer W. D., Young F. E. Edited by Washington, D. C: American Society for Microbiology;
    [Google Scholar]
  5. Chang C. N., Inouye H., Model P., Beckwith J. 1980; Processing of alkaline phosphatase precursor to the mature enzyme by an Escherichia coli inner membrane preparation. Journal of Bacteriology 142:726–728
    [Google Scholar]
  6. Dilworth J. A., Hendley J. O., Mandell G. L. 1975; Attachment and ingestion of gonococci by human neutrophils. Infection and Immunity 11:512–516
    [Google Scholar]
  7. Heckels J. E. 1981; Structural comparison of Neisseria gonorrhoeae outer membrane proteins. Journal of Bacteriology 145:736–742
    [Google Scholar]
  8. Hermodson M. A., Chen K. C. S., Buchanan T. M. 1978; Neisseria pili proteins: amino terminal amino acid sequences and identification of an unusual amino acid. Biochemistry 17:442–445
    [Google Scholar]
  9. Kutsukake K., Iino T. 1980; A trans-acting factor mediates inversion of a specific DNA segment in flagellar phase variation of Salmonella. Nature; London: 284479–481
    [Google Scholar]
  10. Laemmli U. K. 1970; Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature; London: 227680–685
    [Google Scholar]
  11. Lambden P. R., Heckels J. E. 1979; The influence of outer membrane protein composition on the colonial morphology of Neisseria gonorrhoeae strain P9. FEMS Microbiology Letters 5:263–265
    [Google Scholar]
  12. Lambden P. R., Heckels J. E., James L. T., Watt P. J. 1979; Variations in surface protein composition associated with virulence properties in opacity types of Neisseria gonorrhoeae. Journal of General Microbiology 114:305–312
    [Google Scholar]
  13. Lambden P. R., Robertson J. N., Watt P. J. 1980; Biological properties of two distinct pilus types produced by isogenic variants of Neisseria gonorrhoeae P9. Journal of Bacteriology 141:393–396
    [Google Scholar]
  14. Lambden P. R., Heckels J. E., Mcbride H., Watt P. J. 1981a; The identification and isolation of novel pilus types produced by variants of N. gonorrhoeae P9 following selection in vivo. FEMS Microbiology Letters 10:339–341
    [Google Scholar]
  15. Lambden P. R., Robertson J. N., Watt P. J. 1981b; The preparation and properties of α; and β pili from variants of Neisseria gonorrhoeae P9. Journal of General Microbiology 124:109–117
    [Google Scholar]
  16. Ofek L, Beachey E. H., Bisno A. L. 1974; Resistance of Neisseria gonorrhoeae to phagocytosis: relationship to colonial morphology and surface pili. Journal of Infectious Diseases 129:310–316
    [Google Scholar]
  17. Penke B., Ferenczi R., Kovacs K. 1974; A new acid hydrolysis method for determining tryptophan in peptides and proteins. Analytical Biochemistry 60:45–50
    [Google Scholar]
  18. Robertson J. N., Vincent P., Ward M. E. 1977; The preparation and properties of gonococcal pili. Journal of General Microbiology 102:169–177
    [Google Scholar]
  19. Salit I. E., Blake M., Gotschlich E. C. 1980; Intrastrain heterogeneity of gonococcalpili is related to opacity colony variance. Journal of Experimental Medicine 151:716–725
    [Google Scholar]
  20. Swanson J. 1973; Studies on gonococcus infection. IV. Pili: their role in attachment of gonococci to tissue culture cells. Journal of Experimental Medicine 137:571–589
    [Google Scholar]
  21. Wood F. T., Wu M. M., Gerhart J. C. 1975; The radioactive labelling of proteins with an iodinated amidination reagent. Analytical Biochemistry 69:339–349
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-128-9-2105
Loading
/content/journal/micro/10.1099/00221287-128-9-2105
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error