1887

Abstract

Four different types of pili produced by variants of P9 were isolated and characterized. The pili differed in subunit molecular weight with SDS-PAGE and in subunit isoelectric point on agarose gels. Isoelectric points of the major molecular species in Tritonagarose gels of octylglucoside solubilized pili were: , pI 6·5; α;, pI 6·0; , pI 5·3 and γ, pI 5·5. Amino acid analyses of pili showed close homology between different types but a reduction in the content of aspartate and serine was notable in the low molecular weight pili; also and γ pili contained more alanine residues. Structural homology was also demonstrated in peptide maps of tryptic/chymotryptic digests of pili with several major peptides apparently common to all four pilus types.

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/content/journal/micro/10.1099/00221287-128-9-2105
1982-09-01
2021-05-18
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