1887

Abstract

strain S5 (O15 : K:H21) isolated from a septicaemic lamb and previously shown to possess a virulence plasmid, Vir, attached to calf epithelial tissue from the ileum, oesophagus and trachea in the presence of 0·5% (w/v) -mannose. The Vir recombinant strains 711 v and H209av, which had received the Vir plasmid(s) from strain S5, also attached to these epithelia but the parent strains 711 and H209a without the Vir plasmid were non-adhesive. The attachment of the Vir strain 711 v to intestinal brush borders was inhibited by antiserum to live Vir strain H209av but not by antiserum to strain H209a lacking Vir. No adherence occurred with Vir organisms grown at 18 °C or after heating at 65 °C. Adhesion was unaffected by 0·5 % (w/v) formaldehyde. Glucosamine, mannosamine, their -acetyl derivatives and wheat germ lectin each inhibited attachment of Vir strain 711v to brush border epithelia.

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/content/journal/micro/10.1099/00221287-128-9-2097
1982-09-01
2021-10-17
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