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Existing direct isolation methods may seriously underestimate the actual numbers of actinophages in soil, since major ‘losses’ may be incurred during agitation, filter sterilization and, to a lesser extent, centrifugation. Two methods were devised for the isolation and enumeration of soil actinophages and were compared experimentally with two previously described methods. Numbers of up to 2 × 104 p.f.u. (g soil)−1 were detected in compost soil.
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