1887

Abstract

Existing direct isolation methods may seriously underestimate the actual numbers of actinophages in soil, since major ‘losses’ may be incurred during agitation, filter sterilization and, to a lesser extent, centrifugation. Two methods were devised for the isolation and enumeration of soil actinophages and were compared experimentally with two previously described methods. Numbers of up to 2 × 10 p.f.u. (g soil) were detected in compost soil.

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1982-09-01
2024-04-25
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References

  1. Adams M. H. 1948; Surface inactivation of bacterial viruses and of proteins. Journal of General Physiology 31:417–431
    [Google Scholar]
  2. Adams M. H. 1959 In Bacteriophages London: Wiley-Interscience;
    [Google Scholar]
  3. Ball D. F. 1964; Loss-on-ignition as an estimate of organic matter and organic carbon in non-calcareous soils. Journal of Soil Science 15:84–92
    [Google Scholar]
  4. Billing E. 1969; Isolation, growth and preservation of bacteriophages. Methods in Microbiology 3B:315–329
    [Google Scholar]
  5. Bitton G. 1975; Adsorption of viruses on to surfaces in soil and water. Water Research 2:473–484
    [Google Scholar]
  6. Bradley S. G., Anderson D. L., Jones L. A. 1961; Phylogeny of actinomycetes as revealed by susceptibility to actinophage. Developments in Industrial Microbiology 2:223–237
    [Google Scholar]
  7. Burge W. D., Enkiri N. K. 1978; Virus adsorption by five soils. Journal of Environmental Quality 7:73–76
    [Google Scholar]
  8. Carlson G. F., Woodward F. W., Wentworth D. P., Sproul O. J. 1968; Virus inactivation on clay particles in natural waters. Journal of the Water Pollution Control Federation 40:R89–R106
    [Google Scholar]
  9. Casida L. E., Liu L. 1974; Arthrobacter globiformis and its bacteriophage in soil. Applied Microbiology 28:951–959
    [Google Scholar]
  10. Collard C. A. 1970; Comparative studies of methods of isolation of actinophage starting with their natural habitat. Comptesrendus des séances de la Société de Biologic 164:465–468
    [Google Scholar]
  11. Crosse J. E., Hingorani M. K. 1958; A method for isolating Pseudomonas mors-prunorum phages from the soil. Nature; London: 181:60–61
    [Google Scholar]
  12. Day P. R. 1965; Particle Fractionation and Particle-size analysis. In ‘Methods of Soil Analysis’ Agronomy No. 9, Part I pp. 545–567 Edited by Black C. A., Evans D. D., White J. L., Ensminger L. F., Clarke F. E. Madison, Wisconsin: American Society of Agronomy;
    [Google Scholar]
  13. De Vicente J. 1959; A study of the adsorption of bacteriophage by filters. Applied Microbiology 7:239–247
    [Google Scholar]
  14. Dowding J. E. 1973; Characterisation of a bacteriophage virulent for Streptomyces coelicolor A3(2). Journal of General Microbiology 76:163–176
    [Google Scholar]
  15. Dowding J. E., Hopwood D. A. 1973; Temperate bacteriophages for Streptomyces coelicolor A3(2) isolated from soil. Journal of General Microbiology 78:1–11
    [Google Scholar]
  16. Duboise S. M., Moore B. E., Sagik B. P. 1976; Poliovirus survival and movement in a sandy forest soil. Applied and Environmental Microbiology 31:536–543
    [Google Scholar]
  17. Eisenstark A. 1967; Bacteriophage techniques. In ‘Methods in Virology’ I pp. 450–524 Edited by Maramorosch K., Koprowski H. London & New York: Academic Press;
    [Google Scholar]
  18. Gerba C. P., Smith E. M., Melnick J. L. 1977; Development of a quantitative method for detecting enteroviruses in estuarine sediments. Applied and Environmental Microbiology 34:158–163
    [Google Scholar]
  19. Germida J. J., Casida L. E. 1981; Isolation of Arthrobacter bacteriophage from soil. Applied and Environmental Microbiology 41:1389–1393
    [Google Scholar]
  20. Gray T. R. G., Williams S. T. 1971; Microbial productivity in soil. In ‘Microbes and Biological Productivity’ Edited by Hughes E. D., Rose A. Cambridge: Cambridge University Press;
    [Google Scholar]
  21. Hurst C. J., Gerba C. P. 1979; Development of a quantitative method for the detection of enteroviruses in soil. Applied and Environmental Microbiology 37:626–632
    [Google Scholar]
  22. Hurst C. J., Farrah S. R., Gerba C. P., Melnick J. L. 1978; Development of quantitative methods for the detection of enteroviruses in sewage sludge during activation and following land disposal. Applied and Environmental Microbiology 36:81–89
    [Google Scholar]
  23. Jakubowski W. 1969; Adsorption of poliovirus in seawater by clay minerals and marine sediments. Bacteriological ProceedingsV198
    [Google Scholar]
  24. Khavina E. S. 1954; Isolation of actinophage from soils. Trudy Instituta mikrobiologii. Akademiya nauk SSSR 3:224
    [Google Scholar]
  25. Khavina E. S., Rautenstein Y. I. 1959; Effect of pH of the medium on the isolation of actinophages from podzol soil. Microbiology 28:685–691
    [Google Scholar]
  26. Landry E. F., Vaughn J. M., Thomas M. Z., Vicale T. J. 1978; Efficiency of beef extract for the recovery of poliovirus from wastewater effluents. Applied and Environmental Microbiology 36:544–548
    [Google Scholar]
  27. Newbould F. H. S., Garrard E. H. 1954; Studies onactinophage for Streptomyces scabies (Thaxt.) Waksman and Henrici. Canadian Journal of Botany 32:386–391
    [Google Scholar]
  28. Ostle A. G., Holt J. G. 1979; Elution and inactivation of bacteriophages on soil and cation-exchange resin. Applied and Environmental Microbiology 38:59–65
    [Google Scholar]
  29. Ramia S., Sattar S. A. 1979; Second-step concentration of viruses in drinking and surface waters using polyethylene glycol hydro-extraction. Canadian Journal of Microbiology 25:587–592
    [Google Scholar]
  30. Reanney D. C. 1968; An assay for Bacillus stearothermophilus using thermophilic virus. New Zealand Journal of Agricultural Research 11:763–770
    [Google Scholar]
  31. Reanney D. C., Marsh S. C. N. 1973; The ecology of viruses attacking Bacillus stearothermophilus in soil. Soil Biology and Biochemistry 5:399–408
    [Google Scholar]
  32. Retinskaya V. I., Rautenstein Y. I. 1960; The lysogenicity of Actinomyces erythreus cultures and the isolation of their specific actinophages. Microbiology 29:613–617
    [Google Scholar]
  33. Robinson J. B., Corke C. T. 1959; Preliminary studies on the distribution of actinophages in soil. Canadian Journal of Microbiology 5:479–484
    [Google Scholar]
  34. Sykes K. I. 1977 Effects of pH on actinophage Ph.D. thesis, University of Liverpool
    [Google Scholar]
  35. Sykes K. I., Williams S. T. 1978; Interactions of actinophage and clays. Journal of General Microbiology 108:97–102
    [Google Scholar]
  36. Sykes K. I., Lanning S., Williams S. T. 1981; The effect of pH on soil actinophage. Journal of General Microbiology 122:271–280
    [Google Scholar]
  37. Tan J. S. H., Reanney D. C. 1976; Interactions between bacteriophages and bacteria in soil. Soil Biology and Biochemistry 8:145–150
    [Google Scholar]
  38. Troubworst T., Kuyper S., De Jong J. C., Plantinga A. D. 1974; Inactivation of some bacterial and animal viruses by exposure to liquid-air interfaces. Journal of General Virology 24:155–165
    [Google Scholar]
  39. Wellington E. M. H., Williams S. T. 1978; Preservation of actinomycete inoculum in frozen glycerol. Microbios Letters 6:151–157
    [Google Scholar]
  40. Welsch M., Minon A., Schonfield J. K. 1955; Isolation of actinophages. Experientia 11:24–25
    [Google Scholar]
  41. Willoughby L. G., Smith S. M., Bradshaw R. M. 1972; Actinomycete virus in freshwater. Freshwater Biology 2:19–26
    [Google Scholar]
  42. Winer B. J. 1962 Statistical Principles in Experimental Design New York & London: McGraw-Hill;
    [Google Scholar]
  43. Zachary A. 1974; Isolation of bacteriophage of the marine bacterium Beneckea natriegens from coastal salt marshes. Applied Microbiology 27:980–982
    [Google Scholar]
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