@article{mbs:/content/journal/micro/10.1099/00221287-128-9-1981, author = "Mcdonald, I. J. and Calleja, G. B. and Johnson, Byron F.", title = "Conjugation in Chemostat Cultures of Schizosaccharomyces pombe", journal= "Microbiology", year = "1982", volume = "128", number = "9", pages = "1981-1987", doi = "https://doi.org/10.1099/00221287-128-9-1981", url = "https://www.microbiologyresearch.org/content/journal/micro/10.1099/00221287-128-9-1981", publisher = "Microbiology Society", issn = "1465-2080", type = "Journal Article", abstract = "In chemostat cultures, Schizosaccharomyces pombe (NCYC 132 and derivative strains) formed conjugants in glucose-limited but not in ammonia-limited synthetic medium. Conjugation occurred at a low growth rate (doubling time, t d approx. 11 h), low aeration rate (0.1 fermenter vol. air min−1) and 32C, but not at an increased growth rate (t d approx. 4·5 h) or at lower temperatures (20–25 °C). Conjugation of S. pombe 968h90 in chemostat culture was greater under glucose-limitation than ammonia-limitation. Batch cultures of S. pombe 968h90 in synthetic medium produced conjugants when the glucose supply was limited, but when glucose was in excess the amount of nitrogen influenced the degree of conjugation. Conjugation of strain 968h90 was inhibited in batch cultures in synthetic medium under conditions such that a large quantity of glucose was metabolized, and in cultures grown in synthetic medium containing added ethanol (0·5%). Inhibition of conjugation also occurred in batch cultures of S. pombe NCYC 132 and 968h90 in malt extract broth containing added ethanol.", }