1887

Abstract

One hundred and two strains representing the four species of the genus and sixty-four strains representing fourteen other genera of the family Enterobacteriaceae were tested for 192 characters based on their morphology, biochemistry and physiology. The data were subjected to a number of numerical analyses. The results (confirmed by the use of overlap statistics) showed that four phenons can be distinguished within the genus . These correspond with the species and .

Of the other bacteria studied the Alkalescens-Dispar group was most closely related to the genus and the need for this group to be studied further is noted. The importance of using computer-based matrices for the routine identification of strains of and other enterobacteria is discussed.

Strains of the genus clustered separately from the shigellae. Three distinct species were evident within this genus: and a new species. The latter can be equated with the ‘BG3’ group in the DNA-DNA hybridization studies of Brenner (1978) .

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-128-9-1933
1982-09-01
2021-05-11
Loading full text...

Full text loading...

/deliver/fulltext/micro/128/9/mic-128-9-1933.html?itemId=/content/journal/micro/10.1099/00221287-128-9-1933&mimeType=html&fmt=ahah

References

  1. Andrewes F. W., Inman A. C. 1919; A study of the serological races of the Flexner group of dysen-tery bacilli. Medical Research Committee Special Report. Series No. 42 London:
    [Google Scholar]
  2. Baker F. J. 1967 Handbook of Bacteriological Techniques, 2nd edn. London: Butterworths;
    [Google Scholar]
  3. Bascomb S., Lapage S. P., Willcox W. R., Curtis M. A. 1971; Numerical classification of the tribe Klebsiellae. Journal of General Microbiology 66:279–295
    [Google Scholar]
  4. Bascomb S., Lapage S. P., Curtis M. A., Willcox W. R. 1973; Identification of bacteria by computer: identification of reference strains. Journal of General Microbiology 77:291–315
    [Google Scholar]
  5. Brenner D. J., Fanning G. R., Miklos G. V., Steigerwalt A. G. 1973; Polynucleotide sequence relatedness among Shigella species. International Journal of Systematic Bacteriology 23:1–7
    [Google Scholar]
  6. Brenner D. J., Farmer J. J., Fanning G. R., Steigerwalt A. G., Klyken P., Wathen H. G., Hickman F. W., Ewing W. H. 1978; DNA relatedness of Proteus and Providencia species. Inter-national Journal of Systematic Bacteriology 28:269–282
    [Google Scholar]
  7. Carpenter K. P. 1961; The relationship of the Enterobacterium A12 (Sachs) to Shigella boydii 14. Journal of General Microbiology 26:535–542
    [Google Scholar]
  8. Carpenter K. P. 1974; Genus Shigella. In Bergey’s Manual of Determinative Bacteriology, 8th edn. Buchanan R. E., Gibbons N. E. Edited by Baltimore: Williams & Wilkins Co;
    [Google Scholar]
  9. Catalogue Of The National Collection Of Type Cultures 1972 London: HMSO;
  10. Cowan S. T. 1974a In Bergey’s Manual of Determinative Bacteriology, 8th edn. Buchanan R. E., Gibbons N. E. Edited by Baltimore: Williams & Wilkins Co;
    [Google Scholar]
  11. Cowan S. T. 1974b Cowan and Steel’s Manual for the Identification of Medical Bacteria, 2nd edn. Cambridge: Cambridge University Press;
    [Google Scholar]
  12. Craigie J. 1931; Studies on the serological reactions of the flagella of B. typhosus. Journal of Immunology 21:417
    [Google Scholar]
  13. De Assis A. 1948; Shigella guanabara tipo sorologica destacado do grupo do B. ceylonensis-dispar. Hospital Rio de Janeiro 33:505–518
    [Google Scholar]
  14. Edwards P. R., Ewing W. H. 1972 Identification ofEnterobacteriaceae Minneapolis: Burgess Publishing Co;
    [Google Scholar]
  15. Ewing W. H. 1949; Shigella nomenclature. Journal of Bacteriology 57:633–638
    [Google Scholar]
  16. Ewing W. H. 1963; An outline of nomenclature for the family Enterobacteriaceae. International Bulletin of Bacteriological Nomenclature and Taxonomy 13:95–110
    [Google Scholar]
  17. Ewing W. H., Kauffman F. 1950; A new coli O-antigen group. United States Public Health Service Public Health Report 65:1341–1343
    [Google Scholar]
  18. Ewing W. H., Reavis R. W., Davis B. R. 1958; Provisional Shigella serotypes. Canadian Journal of Microbiology 4:89–107
    [Google Scholar]
  19. Feltham R. K. A., Power A. K., Pell P. A., Sneath P. H. A. 1978; A simple method for storage of bacteria at −76 °C. Journal of Applied Bacteriology 44:313–316
    [Google Scholar]
  20. Gekker V. D., Ravitch-Birger E. D., Belaya J. A. 1965; The position of Newcastle bacteria in the classification of the shigellae. International Bulletin of Bacteriological Nomenclature and Taxonomy 15:133–137
    [Google Scholar]
  21. Goullet PH. 1980; Esterase electrophoretic pattern relatedness between Shigella species and Escherichia coli. Journal of General Microbiology 117:493–500
    [Google Scholar]
  22. Gower J. C. 1971; A general coefficient of similarity and some of its properties. Biometrics 27:857–871
    [Google Scholar]
  23. Grimont P. A. D., Dulong De Rosnay H. L. C. 1972; Numerical study of 60 strains of Serratia. Journal of General Microbiology 72:259–268
    [Google Scholar]
  24. Henriksen S. D. 1954; Studies on the Klebsiella group (Kauffmann). II. Biochemical reactions. Acta pathologica et microbiologica scandinavica 34:259–265
    [Google Scholar]
  25. Howie I. S., Old D. C. 1976; A case of severe bacillary dysentery caused by a gas-producing Shigella boydii type 14 (enterobacterium A12 (Sachs)). Medical Laboratory Sciences 33:309–315
    [Google Scholar]
  26. Hugh R., Leifson E. 1953; The taxonomic significance of the fermentative versus oxidative metabolism of carbohydrates by various Gram-negative bacteria. Journal of Bacteriology 66:24–26
    [Google Scholar]
  27. Johnson R., Colwell R. R., Sakazaki R., Tamura K. 1975; Numerical taxonomy study of the Enterobacteriaceae. International Journal of Systematic Bacteriology 25:12–37
    [Google Scholar]
  28. Katzenellenbogen E., Mulczyk M., Roman-Owska C. 1976; Structural studies on O-specific polysaccharide of S. flexneri serotype 6. European Journal of Biochemistry 61:191–197
    [Google Scholar]
  29. Kauffmann F. 1954 Enterobacteriaceae, 2nd edn. Copenhagen : Munksgaard;
    [Google Scholar]
  30. Kovacs N. 1956; Identification of Pseudomonas pyocyanea by the oxidase reaction. Nature; London: 178703
    [Google Scholar]
  31. Manolov D. G. 1959; A new type of the genus Shigella - ‘Shigella 13’. Journal of Hygiene, Epidemiology, Microbiology and Immunology 3:184–190
    [Google Scholar]
  32. Manolov D. G., Trifanova A. 1962 Works of the Research Institute of Epidemiology and Microbiology, Sofia 8:1–8
    [Google Scholar]
  33. Mckell J. 1977 A taxonomic study of the Proteus-Providence group with especial reference to the role of plasmids Ph.D. thesis University of Leicester:
    [Google Scholar]
  34. Mckell J., Jones D. 1976; A numerical taxonomic study of Proteus-Providence bacteria. Journal of Applied Bacteriology 41:143–161
    [Google Scholar]
  35. Mumford E. P., Mohr J. L. 1944; Manual on distribution of communicable diseases and their vectors in tropics. American Journal of Tropical Medicine (Supplement) 24:1–26
    [Google Scholar]
  36. Petrovskaya V. G., Bondarenko V. M. 1977; Recommended corrections to the classification of Shigella flexneri on a genetic basis. International Journal of Systematic Bacteriology 27:171–175
    [Google Scholar]
  37. Ravin A. W. 1963; Experimental approaches to the study of bacterial phylogeny. American Naturalist 97:307–318
    [Google Scholar]
  38. Reavis R. W., Ewing W. H. 1958; Fermentation of raffinose by shigellae. International Bulletin of Bacteriological Nomenclature and Taxonomy 8:75–78
    [Google Scholar]
  39. Regina M., Toledo F., Silva R. M., Trabulsi R. 1981; Sachs’ ‘Enterobacterium A12’ is an aerogenic variant of Shigella boydii 14. International Journal of Systematic Bacteriology 31:242–244
    [Google Scholar]
  40. Report 1951; Nomenclature and classification of the dysentery bacilli. Recommendations of the Shigella Commission (Enterobacteriaceae Subcommittee). International Bulletin of Bacteriological Nomenclature and Taxonomy 1150–166
    [Google Scholar]
  41. Report 1953; Minutes of the Judicial Commission on Bacteriological Nomenclature. International Bulletin of Bacteriological Nomenclature and Taxonomy 3150–151
    [Google Scholar]
  42. Report 1954; Report of the Enterobacteriaceae Sub-committee of the Nomenclature Committee of the International Association of Microbiologists. Second Report of the Shigella Commission. International Bulletin of Bacteriological Nomenclature and Taxonomy 447–94
    [Google Scholar]
  43. Report 1958; Report of the Enterobacteriaceae Sub-committee of the Nomenclature Committee of the International Association of Microbiological Soci-eties. Supplement to the third report of the Shigella group. International Bulletin of Bacteriological Nomenclature and Taxonomy 893–95
    [Google Scholar]
  44. Rowe B., Gross R. J., Woodroof D. P. 1977; Proposal to recognize serovar 145/146 (synonyms: 147, Shigella 13, Shigella Sofia and Shigella manolovii) as a new E. coli O Group, 0164;. International Journal of Systematic Bacteriology 27:15–18
    [Google Scholar]
  45. Rubinsten S., PiÉchaud D., Kirsche P. 1950; Shigella saigonensis a propos de treize souches récemment isolees. Annales d’Institut Pasteur 79:910–913
    [Google Scholar]
  46. Serény B. 1967; Identification of shigellae on the basis of selective sensitivity. Acta microbiologica Academia scientiarium hungaricae 14:375–380
    [Google Scholar]
  47. Simmons D. A. R. 1971; Immunochemistry of Shigella flexneri O-antigens: a study of structural and genetic aspects of the biosynthesis of cell-surface antigens. Bacteriological Reviews 35:117–148
    [Google Scholar]
  48. Skerman V. B. D., Mcgowan V., Sneath P. H. A. 1980; Approved lists of bacterial names. International Journal of Systematic Bacteriology 30:225–420
    [Google Scholar]
  49. Slopek S., Mulczyk M. 1967; Concerning the classification of S. flexneri 6 bacilli. Archivum Immunologiae et Therapiae Experimentalis 15:600–603
    [Google Scholar]
  50. Smith P. B., Hancock G. A., Rhoden D. L. 1969; Improved medium for detecting deoxyribonuclease-producing bacteria. Applied Microbiology 18:991–993
    [Google Scholar]
  51. Sneath P. H. A. 1968; Vigour and pattern in taxonomy. Journal of General Microbiology 54:1–11
    [Google Scholar]
  52. Sneath P. H. A. 1979; Basic program for significance test for two clusters in euclidean space as measured by their overlap. Computers and Geosciences 5:143–155
    [Google Scholar]
  53. Sneath P. H. A., Johnson R. 1972; The influence on numerical taxonomic studies of errors in micro-biological tests. Journal of General Microbiology 72:377–392
    [Google Scholar]
  54. Sneath P. H. A., Sokal R. R. 1973 Numerical Taxonomy San Francisco: Freeman & Co;
    [Google Scholar]
  55. Stevens M. 1969; Development and use of multi-inoculation test methods for a taxonomic study. Journal of Medical Laboratory Technology 26:253–263
    [Google Scholar]
  56. Szturm-Rubinsten S., PiÉchaud D., Allos G. 1962; A propos d’une varieté gazogène de Shigella boydii 14. Annales d’Institut Pasteur 103:303–305
    [Google Scholar]
  57. Tekelieva R. 1975; Immunochemical studies of the lipopolysaccharides of ‘serotype 145–146’ (Enterobacteriaceae). Zentralblatt für Bakteriologie, Parasitenkunde, Infektionskrankheiten und Hygiene (Abteilung I) 231:92–96
    [Google Scholar]
  58. Timakov V. D., Petrovskaya V. G., Bondarenko V. M., Khomenko N. A. 1972; Genetic data concerning Shigella flexneri serotypes 5 and 6. Inter-national Journal of Systematic Bacteriology 22:149–154
    [Google Scholar]
  59. Véron M. 1975; Nutrition and taxonomy of ‘Enterobacteriaceae’ and related bacteria. I. Technical procedure for auxanograms. Annales de Microbiologie 126:267–274
    [Google Scholar]
  60. Véron M., Le Minor L. 1975; Nutrition and taxonomy of ‘Enterobacteriaceae’ and related bacteria. II. General results and classification. Annales de Microbiologie 126B:111–124
    [Google Scholar]
  61. Weil A. J., De Assis A., Slafkovsky H. 1948; Shigella rio, a new type of Shigella. Journal of Immunology 58:23–26
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-128-9-1933
Loading
/content/journal/micro/10.1099/00221287-128-9-1933
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error