1887

Abstract

Under sporulation conditions, mutant SE63 secreted into the medium an inhibitor of the extracellular DNAase that is specifically associated with stage II to III of sporulation. The inhibitor was a heat-labile protein with a molecular weight of 18000–20000. A cell-bound, presumably intracellular, inhibitor with the same properties was found in extracts of wild-type cells and of the mutant. The mechanisms by which the mutation, designated , might cause the secretion of a protein that is normally intracellular are discussed. The mutation was mapped by transduction with phage PBS1 and it lies between and . It was also introduced into a variety of sporulation mutants including some blocked at the earliest known stages of the process. In all of these it caused secretion of the inhibitor. It is concluded that the secretion is therefore not integrally associated with sporulation but is, instead, part of the response of the cells to the nutritional step-down conditions in which spore formation is induced.

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-128-8-1903
1982-08-01
2021-10-16
Loading full text...

Full text loading...

/deliver/fulltext/micro/128/8/mic-128-8-1903.html?itemId=/content/journal/micro/10.1099/00221287-128-8-1903&mimeType=html&fmt=ahah

References

  1. Akrigg A. 1978; Purification and properties of a manganese-stimulated deoxyribonuclease produced during sporulation of Bacillus subtilis. Biochemical Journal 172:69–76
    [Google Scholar]
  2. Akrigg A., Mandelstam J. 1978; Extracellular manganese-stimulated deoxyribonuclease as a marker event in sporulation of Bacillus subtilis. Biochemical Journal 172:63–67
    [Google Scholar]
  3. Andrews P. 1964; Estimation of molecular weights of proteins by Sephadex gel-filtration. Biochemical Journal 91:222–233
    [Google Scholar]
  4. Bassford P., Beckwith J. 1979; Escherichia coli mutants accumulating the precursor of a secreted protein in the cytoplasm. Nature; London: 277538–541
    [Google Scholar]
  5. Chestukhin A. V., Fedchenko V. I., Shemyakin M. E. 1979; Isolation and properties of DNA-binding proteins of Bacillus subtilis inhibiting ATP-dependent deoxyribonuclease. Molecular Biology 13:656–665
    [Google Scholar]
  6. Coote J. G. 1972; Sporulation in Bacillus subtilis.Characterization of oligosporogenous mutants and comparison of their phenotypes with those of asporogenous mutants. Journal of General Microbiology 71:1–15
    [Google Scholar]
  7. Davis B. D., Tai P.-C. 1980; The mechanism of protein secretion across membranes. Nature; London: 283433–438
    [Google Scholar]
  8. Davis B. J. 1964; Disc electrophoresis. II. Method and application to human serum proteins. Annals of the New York Academy of Sciences 121:404–427
    [Google Scholar]
  9. Emr S. D., Schwartz M., Silhavy T. J. 1978; Mutations altering the cellular localization of the phage lambda receptor, an Escherichia coli outer membrane protein. Proceedings of the National Academy of Sciences of the United States of America 75:5802–5806
    [Google Scholar]
  10. Ephrati-Elizur E. 1968; Spontaneous transformation in Bacillus subtilis. Genetical Research 11:83–96
    [Google Scholar]
  11. Gay P., Cordier P., Marquet M., Delobbe A. 1973; Carbohydrate metabolism and transport in Bacillus subtilis. A study of ctr mutations. Molecular and General Genetics 121:355–368
    [Google Scholar]
  12. Glenn A. R., Mandelstam J. 1971; Sporulation in Bacillus subtilis 168. Comparison of alkaline phosphatase from sporulating and vegetative cells. Biochemical Journal 123:129–138
    [Google Scholar]
  13. Horney D. L., Webster D. A. 1971; Deoxy-ribonuclease: a sensitive assay using radial diffusion in agarose containing methyl green-DNA complex. Biochimica et biophysica acta 247:54–61
    [Google Scholar]
  14. Hranueli D., Piggot P. J., Mandelstam J. 1974; Statistical estimate of the total number of operons specific for Bacillus subtilis sporulation. Journal of Bacteriology 119:684–690
    [Google Scholar]
  15. Jenkinson H. F., Sawyer W. D., Mandelstam J. 1981; Synthesis and order of assembly of spore coat proteins in Bacillus subtilis. Journal of General Microbiology 123:1–16
    [Google Scholar]
  16. Laemmli U. K., Favre M. 1973; Maturation of the head of bacteriophage T4. I. DNA packaging events. Journal of Molecular Biology 80:575–599
    [Google Scholar]
  17. Mandelstam J. 1976; Bacterial sporulation: a problem in the biochemistry and genetics of a primitive developmental system. Proceedings of the Royal Society B193:89–106
    [Google Scholar]
  18. Millet J. 1970; Characterization of proteinases excreted by Bacillus subtilis Marburg strain during sporulation. Journal of Applied Bacteriology 33:207–219
    [Google Scholar]
  19. Oakley B. R., Kirsch D. R., Morris N. R. 1980; A simplified ultrasensitive silver stain for detecting proteins in polyacrylamide gels. Analytical Biochemistry 105:361–363
    [Google Scholar]
  20. Piggot P. J. 1973; Mapping of asporogenous mutants of Bacillus subtilis: a minimum estimate of the number of sporulation operons. Journal of Bacteriology 114:1241–1253
    [Google Scholar]
  21. Piggot P. J., Coote J. G. 1976; Genetic aspects of bacterial endospore formation. Bacteriological Reviews 40:908–962
    [Google Scholar]
  22. Porter A. C. G. 1981; Use of methyl green-DNA agarose for detecting deoxyribonuclease activity in polyacrylamide gels. Analytical Biochemistry 117:28–31
    [Google Scholar]
  23. Rosenthal A. L., Lacks S. A. 1977; Nuclease detection in SDS-polyacrylamide gel electrophoresis. Analytical Biochemistry 80:76–90
    [Google Scholar]
  24. Schaeffer P. 1969; Sporulation and the production of antibiotics, exoenzymes and exotoxins. Bacteriological Reviews 33:48–71
    [Google Scholar]
  25. Shapiro A. L., Viáuela E., Maizel J. V. 1967; Molecular weight estimation of polypeptide chains by electrophoresis in SDS-polyacrylamide gels. Biochemical and Biophysical Research Communications 28:815–820
    [Google Scholar]
  26. Staal S. P., Hoch J. A. 1972; Conditional dihydrostreptomycin resistance in Bacillus subtilis. Journal of Bacteriology 110:202–207
    [Google Scholar]
  27. Sterlini J. M., Mandelstam J. 1969; Commitment to sporulation in B. subtilis and its relationship to the development of actinomycin resistance. Biochemical Journal 113:29–37
    [Google Scholar]
  28. Waites W. M., Kay D., Dawes I. W., Wood D. A., Warren S. C., Mandelstam J. 1970; Sporulation in Bacillus subtilis. Correlation of biochemical events with morphological changes in asporogenous mutants. Biochemical Journal 118:667–676
    [Google Scholar]
  29. Yoneda Y., Yamane K., Maruo B. 1973; Membrane mutation related to the production of extracellular a-amylase and protease in Bacillus subtilis. Biochemical and Biophysical Research Communications 50:765–770
    [Google Scholar]
  30. Young M., Mandelstam J. 1979; Early events during bacterial endospore formation. Advances in Microbial Physiology 20:103–162
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-128-8-1903
Loading
/content/journal/micro/10.1099/00221287-128-8-1903
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error