1887

Abstract

SUMMARY: A plasmid (pSV1) of 110 x 10 daltons from a methylenomycin A producing strain of was detected on and isolated from agarose gels. Elimination of this plasmid by protoplasting and regeneration resulted in the simultaneous loss of methylenomycin A production and resistance. pSV1 hybridized with pBR322 containing a cloned fragment of 1.7 x 10 daltons from A3(2) which codes for methylenomycin A resistance. The pSV1 plasmid could be transferred to by conjugation and by transformation and plasmid DNA identical in size to pSV1 could be isolated from the recipient strains. These experiments show that pSV1 codes for methylenomycin A production and resistance, in close analogy to the SCP1 plasmid from A3(2).

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-128-8-1893
1982-08-01
2019-10-17
Loading full text...

Full text loading...

http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-128-8-1893
Loading
This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error