1887

Abstract

mutant of with undetectable phosphoribosylpyrophosphate (PRPP) synthetase activity and abnormally low PRPP pools was identified by screening temperature-sensitive isolates by an autoradiographic procedure. The lack of PRPP synthetase activity and temperature-sensitive growth were shown to result from separate, but closely linked mutations mapping at 47 units on the chromosome. Mutant cell extracts prepared by a variety of methods did not show any detectable PRPP synthetase activity, but material that was immunochemically cross-reactive with PRPP synthetase was detected by complement fixation analysis. A second mutant, isolated by localized mutagenesis, contained about half the PRPP synthetase and cross-reacting material of the parental strain.

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/content/journal/micro/10.1099/00221287-128-8-1863
1982-08-01
2021-08-03
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References

  1. Bagnara A. S., Finch L. R. 1974; The effects of bases and nucleosides on the intracellular contents of nucleotides and 5-phosphoribosyl-1-pyrophosphate in Escherichia coli. European Journal of Biochemistry 41:421–430
    [Google Scholar]
  2. Brenchley J., Ingraham J. L. 1973; Characterization of cold-sensitive hisW mutant of Salmonella typhimurium. Journal of Bacteriology 114:528–536
    [Google Scholar]
  3. Burton K. 1977; Transport of adenine, hypoxanthine, and uracil into Escherichia coli. Biochemical Journal 168:195–204
    [Google Scholar]
  4. Clark D. J., Maaloe O. 1967; DNA replication and the division cycle in Escherichia coli. Journal of Molecular Biology 23:99–112
    [Google Scholar]
  5. Crowle A. J. 1960 Immunodiffusion New York: Academic Press.;
    [Google Scholar]
  6. Hane M. W. 1971; Some effects of nalidixic acid on conjugation in Escherichia coli K12. Journal of Bacteriology 105:46–55
    [Google Scholar]
  7. Hochstadt J. 1974; The role of the membrane in the utilization of nucleic acid precursors. CRC Critical Reviews in Biochemistry 2:259–310
    [Google Scholar]
  8. Hochstadt J., Stadtman E. R. 1971; The regulation of purine utilization in bacteria. III. The involvement of purine phosphoribosyltransferase in the uptake of adenine and other nucleic acid precursors by intact resting cells. Journal of Biological Chemistry 246:5312–5320
    [Google Scholar]
  9. Hong J., Ames B. N. 1971; Localized mutagenesis of any specific small region of the bacterial chromosome. Proceedings of the National Academy of Sciences of the United States of America 68:3158–3162
    [Google Scholar]
  10. Jensen K. F., Houlberg I., Nygaard P. 1979; Thin-layer chromatographic methods to isolate 32P-labeled 5-phosphoribosyl-α-1-pyrophosphate (PRPP): determination of cellular PB.PP pools and assay of PRPP synthetase activity. Analytical Biochemistry 98:254–263
    [Google Scholar]
  11. Jochimsen B., Garber B., Gots J. S. 1980; Phosphoribosylpyrophosphate synthetase mutant in Salmonella typhimurium. Advances in Experimental Medicine and Biology 122B:131–136
    [Google Scholar]
  12. Laemmli U. K., Favre M. 1973; Maturation of the head of bacteriophage T4. Journal of Molecular Biology 80:575–599
    [Google Scholar]
  13. Levine L., Van Vunakis H. 1967; Microcomplement fixation. Methods in Enzymology 40:928–936
    [Google Scholar]
  14. Munch-Petersen A., Mygind B., Nicolaisen A., Phil N. J. 1979; Nucleoside transport in cells and membrane vesicles from Escherichia coli K12. Journal of Biological Chemistry 254:3730–3737
    [Google Scholar]
  15. Olszowy J., Switzer R. L. 1972; Specific repression of phosphoribosylpyrophosphate synthetase by uridine compounds in Salmonella typhimurium. Journal of Bacteriology 110:450–451
    [Google Scholar]
  16. Page M. G. P., Burton K. 1978; The location of phosphoribosyl-transferase activities in Escherichia coli. Biochemical Journal 174:717–725
    [Google Scholar]
  17. Riddle D. L., Roth J. R. 1972; Frameshift suppressors. II. Genetic mapping and dominance studies. Journal of Molecular Biology 66:483–493
    [Google Scholar]
  18. Roth J. 1970; Genetic techniques in studies of bacterial metabolism. Methods of Enzymology 17A:3–35
    [Google Scholar]
  19. Roy-Burman S., Visser D. W. 1975; Transport of purines and deoxyadenosine in Escherichia coli. Journal of Biological Chemistry 250:9270–9275
    [Google Scholar]
  20. Sadler W. C., Switzer R. L. 1977; Regulation of Salmonella phosphoribosylpyrophosphate synthetase activity in vivo. Deductions from pool measurements. Journal of Biological Chemistry 252:8504–8511
    [Google Scholar]
  21. Sanderson K. E., Hartman P. E. 1978; Linkage map of Salmonella typhimurium, edition V. Microbiological Reviews 42:471–519
    [Google Scholar]
  22. Sanderson K. E., Ross H., Zlegler L., Makela P. H. 1972; F+, Hfr, and F′ strains of Salmonella typhimurium and Salmonella abony. Bacteriological Reviews 36:608–637
    [Google Scholar]
  23. Schubert K. R., Switzer R. L. 1975; Studies of the quaternary structure and the chemical properties of phosphoribosylpyrophosphate synthetase from Salmonella typhimurium. Journal of Biological Chemistry 250:7492–7500
    [Google Scholar]
  24. Switzer R. L., Gibson K. J. 1978; Phosphoribosylpyrophosphate synthetase (ribose-5-phosphate pyrophosphokinase) from Salmonella typhimurium. Methods in Enzymology 50:3–11
    [Google Scholar]
  25. Switzer R. L., Sogin D. C. 1973; Regulation and mechanism of phosphoribosylpyrophosphatesynthetase. V. Inhibition by end products and regulation by adenosine diphosphate. Journal of Biological Chemistry 248:1063–1073
    [Google Scholar]
  26. Switzer R. L., Turnbough C.L, Brabson J. S., Waindle L. M. 1975; Enzyme inactivation of de novo nucleotide biosynthesis in sporulating Bacillus subtilis cells. In Spores VI pp. 327–334 Gerhardt P., Costilow R. N., Sadoff H. L. Edited by Washington, D.C.: American Society for Microbiology.;
    [Google Scholar]
  27. Vogel H. J., Bonner D. M. 1956; Acetyl ornithinase of Escherichia coli: partial purification and some properties. Journal of Biological Chemistry 218:97–106
    [Google Scholar]
  28. White M. N., Olszowy J., Switzer R. L. 1971; Regulation and mechanism of phosphoribosylpyrophosphate synthetase: repression by end products. Journal of Bacteriology 108:122–131
    [Google Scholar]
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