1887

Abstract

SUMMARY: A mutant of with undetectable phosphoribosylpyrophosphate (PRPP) synthetase activity and abnormally low PRPP pools was identified by screening temperature-sensitive isolates by an autoradiographic procedure. The lack of PRPP synthetase activity and temperature-sensitive growth were shown to result from separate, but closely linked mutations mapping at 47 units on the chromosome. Mutant cell extracts prepared by a variety of methods did not show any detectable PRPP synthetase activity, but material that was immunochemically cross-reactive with PRPP synthetase was detected by complement fixation analysis. A second mutant, isolated by localized mutagenesis, contained about half the PRPP synthetase and cross-reacting material of the parental strain.

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/content/journal/micro/10.1099/00221287-128-8-1863
1982-08-01
2020-11-23
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http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-128-8-1863
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