1887

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Volume 128, Issue 8

Magnesium-limited Growth of the Cyanobacterium Free

Abstract

SMg-limited growth of the cyanobacterium was investigated in batch and chemostat cultures. In batch cultures the growth rate of the organism depended on the Mg concentration up to 5 μ. Although the maximum growth rate was achieved at this concentration, the organism formed aseptate filaments of three to four times the‘normal’ cell length. About 90 min after increasing the Mg concentration from 5 μ to 1 m the cell size decreased, followed by an increase in the division rate, which lasted for about 60 min and resulted in a 66% increase in cell number. The rates of DNA, RNA and protein synthesis were not altered during these Mg shift-up experiments, showing that the control by Mg of growth had been separated from its control of cell division, In Mg-limited chemostat cultures, the mean cell volume decreased from about 2·0 to 0·6 μm when the Mg concentration was increased from 2·5 to 10 μ. This increase in Mg also resulted in an increase in the calculated intracellular Mg concentration from 27 to 78 m, and the amount of cellular Mg bound in chlorophyll increased from 17 to 22%. A comparison of Mg-and SO -limited chemostat cultures showed that the mean cell volume decreased with increasing dilution rate when Mg was the limiting factor, whereas it increased with dilution rate when SO was limiting. Only small differences in the rates of RNA and protein synthesis were found in the two cultures, although the synthesis of RNA was Mg-dependent. The ratio of total RNA to protein, which gives the amount of RNA necessary to synthesize one protein unit (RNA efficiency), was independent of the growth rate in both SO -and Mg-limited chemostat cultures showing that the efficiency of culture RNA was variable in both cases. The efficiency was higher under SO -than Mg-limited conditions.

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© Society for General Microbiology 1982
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1982-08-01
2024-04-19
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References

  1. Ahluwalia B., Duffus J. H., Paterson L. J., Walker G. M. 1978; Synchronization of cell division in the fission yeast Schizosaccharomyces pombe by ethylenediaminetetra-acetic acid. Journal of General Microbiology 106:261–264
     [Google Scholar]
  2. Allen M. M. 1968; Simple conditions for growth of blue-green algae on plates. Journal of Phycology 4:1–4
     [Google Scholar]
  3. Braun V. 1975; Covalent lipoprotein from the outer membrane of Escherichia coli. Biochimica et biophysica acta 415:335–377
     [Google Scholar]
  4. Brock T. D. 1962; Effects of magnesium ion deficiency on Escherichia coli and possible relation to the mode of action of novobiocin. Journal of Bacteriology 84:679–682
     [Google Scholar]
  5. Burton K. 1956; A study of the conditions and mechanisms of the colorimetric estimation of deoxyribonucleic acid. Biochemical Journal 62:315–322
     [Google Scholar]
  6. Carr N. G., Whitton B. A. 1973 The Biology of Blue-Green Algae. Oxford: Blackwell Scientific Publications;
     [Google Scholar]
  7. Dixon M., Webb E. C. 1958 Enzymes. London: Longmans, Green and Co.;
     [Google Scholar]
  8. Duffus J. H., Paterson L. J. 1974; Control of cell division in yeast using the ionophore A23187 with calcium and magnesium. Nature; London: 251626–627
     [Google Scholar]
  9. Finkel B. I., Appleman D. 1953; The role of magnesium concentration on the growth of Chlorella. Plant Physiology 28:652–663
     [Google Scholar]
  10. Garrett A. J. 1969; The effect of magnesium ion deprivation on the synthesis of mucopeptide and its precursors in Bacillus subtilis. Biochemical Journal 115:419–430
     [Google Scholar]
  11. Golecki J. R. 1977; Studies on ultrastructure and composition of cell walls of the cyanobacterium Anacystis nidulans. Archives of Microbiology 114:35–41
     [Google Scholar]
  12. Grunberg-Manago M., Buckingham R. H., Cooperman B. S., Hershey J. W. B. 1978; Structure and function of the translation machinery. Symposia of the Society for General Microbiology 28:26–110
     [Google Scholar]
  13. Herbert D., Elsworth R., Telling R. C. 1956; The continuous culture of bacteria; a theoretical and experimental study. Journal of General Microbiology 14:601–622
     [Google Scholar]
  14. Herbert D., Phipps P. J., Strange R. E. 1971; Chemical analysis of microbial cells. Methods in Microbiology 5B:209–344
     [Google Scholar]
  15. Jasper P., Silver S. 1977; Magnesium transport in microorganisms. In Microorganisms and Minerals pp. 7–47 Weinberg E. P. Edited by New York: Marcel Dekker;
     [Google Scholar]
  16. Kennell D. E. 1967; Nucleic acid and protein metabolism in Aerobacter aerogenes during magnesium starvation. In Microbial Physiology and Continuous Culture Proceedings of the Third International Symposium pp. 76–99 Powell E. O., Evans C. G. T., Strange R. E., Tempest D. W. Edited by London: HMSO;
     [Google Scholar]
  17. Koch A. L. 1971; The adaptive responses of Escherichia coli to a feast and famine existence. Advances in Microbial Physiology 6:147–217
     [Google Scholar]
  18. Kratz W. A., Myers J. 1955; Nutrition and growth of several blue-green algae. American Journal of Botany 42:282–287
     [Google Scholar]
  19. Maalöe O., Kjeldgaard N. O. 1966 Control of Macromolecular Synthesis. New York: W. A. Benjamin;
     [Google Scholar]
  20. Mann N., Carr N. G. 1977; Coupling between the initiation of DNA replication and cell division in the blue-green alga Anacystis nidulans. Archives of Microbiology 112:95–98
     [Google Scholar]
  21. Myers J., Kratz W. A. 1956; Relations between pigment content and photosynthetic characteristics in a blue-green alga. Journal of General Physiology 39:11–22
     [Google Scholar]
  22. Parsons T. R., Strickland J. D. H. 1963; Discussion of spectrophotometric determination of marine plant-pigments, with revised equations for ascertaining chlorophylls and carotenoids. Journal of Marine Research 21:153–163
     [Google Scholar]
  23. Retovsky R., Klasterska I. 1961; Study of the growth and development of Chlorella populations in the culture as a whole. V. The influence of MgSO4on autospore formation. Folia microbiologica 6:115–126
     [Google Scholar]
  24. Rogers H. J., Reaveley D. A., Burdett I. D. J. 1967 Protides of the Biological Fluids 15 pp. 303–313 Peeters H. Edited by Amsterdam: Elsevier;
     [Google Scholar]
  25. Strange R. E., Hunter J. R. 1967; Effect of magnesium on survival of bacteria in aqueous suspension. In Microbial Physiology and Continuous Culture Proceedings of the Third International Symposium pp. 102–123 Powell E. O., Evans C. G. T., Strange R. E., Tempest D. W. Edited by London: HMSO;
     [Google Scholar]
  26. Sykes J. 1967; Ribosomes and ribonucleic acid from bacteria grown in defined environments. In Microbial Physiology and Continuous Culture Proceedings of the Third International Symposium pp. 124–139 Powell E. O., Evans C. G. T., Strange R. E., Tempest D. W. Edited by London: HMSO;
     [Google Scholar]
  27. Tempest D. W., Dicks J. W. 1967; Interrelationships between potassium, magnesium, phosphorus and ribonucleic acid in the growth of Aerobacter aerogenes in a chemostat. In Microbial Physiology and Continuous Culture Proceedings of the Third International Symposium pp. 140–154 Powell E. O., Evans C. G. T., Strange R. E., Tempest D. W. Edited by London: HMSO;
     [Google Scholar]
  28. Tempest D. W., Hunter J. R. 1965; The influence of temperature and pH value on the macromolecular composition of magnesium-limited and glycerol-limited Aerobacter aerogenes growing in a chemostat. Journal of General Microbiology 41:267–273
     [Google Scholar]
  29. Tempest D. W., Strange R. E. 1966; Variation in content and distribution of magnesium, and its influence on survival, in Aerobacter aerogenes grown in the chemostat. Journal of General Microbiology 44:273–279
     [Google Scholar]
  30. Tempest D. W., Hunter J. R., Sykes J. 1965; Magnesium-limited growth of Aerobacter aerogenes in a chemostat. Journal of General Microbiology 39:355–366
     [Google Scholar]
  31. Tissiéres A., Watson J. D. 1958; Ribonucleoprotein particles from Escherichia coli. Nature; London: 182778–780
     [Google Scholar]
  32. Torti S., Park I. T. 1976; Lipoprotein of Gram-negative bacteria is essential for growth and division. Nature; London: 263323–326
     [Google Scholar]
  33. Walker G. M., Duffus J. H. 1980; Magnesium ions and the control of the cell cycle in yeast. Journal of Cell Science 42:329–356
     [Google Scholar]
  34. Webb M. 1949; The influence of magnesium on cell division. 2. The effect of magnesium on the growth and cell division of various bacterial species in complex media. Journal of General Microbiology 3:410–417
     [Google Scholar]
  35. Weibull C. 1956; Bacterial protoplasts; their formation and characteristics. Symposia of the Society for General Microbiology 6:111–126
     [Google Scholar]
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Magnesium-limited Growth of the Cyanobacterium Anacystis nidulans
Microbiology 128, 1849 (1982); https://doi.org/10.1099/00221287-128-8-1849
/content/journal/micro/10.1099/00221287-128-8-1849
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