1887

Abstract

Transfer of plasmid pIJ1008, a recombinant of two indigenous plasmids into strain 300 produced strain 3960, which reduced significantly more N in pea root nodules than did strain 300 itself. Strain 3960 was superior to the field isolate 128C53, from which the symbiotic determinants of pIJ1008 were derived, and transfer of plasmid pIJ1008 into two other genetic backgrounds also improved symbiotic performance compared to the introduction of other nodulation plasmids.

As plasmid pIJ1008 carries genetic determinants for an uptake hydrogenase activity (Hup) as well as nodulation capability (Nod) and other determinants for symbiotic nitrogen fixation (Fix), the increased effectiveness of strains carrying pIJ1008 may result from their capacity to conserve energy by recovering H evolved by nitrogenase.

Intact plant studies with N showed that the superior N fixation capability associated with pIJ1008 was enhanced by 2 m-NO , a common concentration of soil N. It was also shown that, as plants grew older, the hydrogenase determined by pIJ1008 was not able to recycle all the hydrogen evolved by pea root nodules.

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-128-8-1829
1982-08-01
2024-04-16
Loading full text...

Full text loading...

/deliver/fulltext/micro/128/8/mic-128-8-1829.html?itemId=/content/journal/micro/10.1099/00221287-128-8-1829&mimeType=html&fmt=ahah

References

  1. Albrecht S. L., Maier R. J., Hanus F. J., Russell S. A., Emerich D. W., Evans H. J. 1979; Hydrogenase in Rhizobium japonicum increases nitrogen fixation by nodulated soybeans. Science 203:1255–1257
    [Google Scholar]
  2. Beringer J. E. 1974; R factor transfer in Rhizobium leguminosarum. . Journal of General Microbiology 84:188–198
    [Google Scholar]
  3. Beringer J. E., Hoggan S. A., Johnston A. W. B. 1978; Linkage mapping in Rhizobium leguminosarum by means of R plasmid-mediated recombination. Journal of General Microbiology 104:201–207
    [Google Scholar]
  4. Bethlenfalvay G. J., Phillips D. A. 1977; Ontogenetic interactions between photosynthesis and symbiotic nitrogen fixation in legumes. Plant Physiology 60:419–421
    [Google Scholar]
  5. Bethlenfalvay G. J., Phillips D. A. 1979; Variation in nitrogenase and hydrogenase activity of Alaska pea root nodules. Plant Physiology 63:816–820
    [Google Scholar]
  6. Bethlenfalvay G. J., Abu-Shakra S. S., Phillips D. A. 1978; Interdependence of nitrogen nutrition and photosynthesis in Pisum sativum L. II. Host plant response to nitrogen fixation by Rhizobium strains. Plant Physiology 62:131–134
    [Google Scholar]
  7. Brewin N. J., Beringer J. E., Buchanan-Wollaston A. V., Johnston A. W. B., Hirsch P. R. 1980a; Transfer of symbiotic genes with bacteriocinogenic plasmids in Rhizobium leguminosarum. . Journal of General Microbiology 116:216–270
    [Google Scholar]
  8. Brewin N. J., Beringer J. E., Johnston A. W. B. 1980b; Plasmid mediated transfer of host-range specificity between two strains of Rhizobium leguminosarum. . Journal of General Microbiology 120:413–420
    [Google Scholar]
  9. Brewin N. J., DeJong T. M., Phillips D. A., Johnston A. W. B. 1980c; Co-transfer of determinants for hydrogenase activity and nodulation ability in Rhizobium leguminosarum. . Nature London: 288:77–79
    [Google Scholar]
  10. Brewin N. J., Wood E. A., Johnston A. W. B., Dibb N. J., Hombrecher G. 1982; Recombinant nodulation plasmids in Rhizobium leguminosarum. . Journal of General Microbiology 128:1817–1827
    [Google Scholar]
  11. Bulen W. A., Lecomte J. R. 1966; The nitrogenase system from Azotobacter: two enzyme requirements for N2 reduction, ATP-dependent H2 evolution, and ATP hydrolysis. Proceedings of the National Academy of Sciences of the United States of America 56:979–986
    [Google Scholar]
  12. Burris R. H., Wilson P. W. 1957; Methods for measurement of nitrogen fixation. Methods in Enzymology 4:355–366
    [Google Scholar]
  13. DeJong T. M., Brewin N. J., Phillips D. A. 1981; Effect of plasmid content in Rhizobium leguminosarum on symbiosis and host plant functions. Journal of General Microbiology 124:1–7
    [Google Scholar]
  14. DeJong T. M., Phillips D. A. 1981; Nitrogen stress and apparent photosynthesis in symbiotically-grown Pisum sativum L. Plant Physiology 68:309–313
    [Google Scholar]
  15. Dixon R. O. D. 1967; Hydrogen uptake and exchange by pea root nodules. Annals of Botany 31:179–188
    [Google Scholar]
  16. Emerich D. W., Ruiz-Argüeso T., Ching T. M., Evans H. J. 1979; Hydrogen-dependent nitrogenase activity and ATP formation in Rhizobium japonicum bacteroids. Journal of Bacteriology 137:153–160
    [Google Scholar]
  17. Hirsch P. R., Johnston A. W. B., Brewin N. J., VanMontagu M., Schell J. 1980; Physical identification of bacteriocinogenic, nodulation and other plasmids in strains of Rhizobium leguminosarum. . Journal of General Microbiology 120:403–412
    [Google Scholar]
  18. Hombrecher G., Brewin N. J., Johnston A. W. B. 1981; Linkage of genes for nitrogenase and nodulation ability on plasmids in Rhizobium leguminosarum and R. phaseoli. . Molecular and General Genetics 182:133–136
    [Google Scholar]
  19. Johnston A. W. B., Beringer J. E. 1975; Identification of the Rhizobium strains in pea root nodules using genetic markers. Journal of General Microbiology 87:343–350
    [Google Scholar]
  20. Johnston A. W. B., Beynon J. L., Buchanan-Wollaston A. V., Setchell S. M., Hirsch P. R., Beringer J. E. 1978; High frequency transfer of nodulating ability between strains and species of Rhizobium. . Nature London: 276:635–636
    [Google Scholar]
  21. Johnston A. W. B., Hombrecher G., Brewin N. J., Cooper M. C. 1982; Two transmissible plasmids in Rhizobium leguminosarum strain 300. Journal of General Microbiology 128:85–93
    [Google Scholar]
  22. Josey D. P., Beynon J. L., Johnston A. W. B., Beringer J. E. 1979; Strain identification in Rhizobium using intrinsic antibiotic resistance. Journal of Applied Bacteriology 46:343–350
    [Google Scholar]
  23. Leonard L. T. 1943; A simple assembly for use in testing of cultures of rhizobia. Journal of Bacteriology 45:523–527
    [Google Scholar]
  24. Maier R. J., Brill W. J. 1978; Mutant strains of Rhizobium japonicum with increased ability to fix nitrogen for soybean. Science 201:448–450
    [Google Scholar]
  25. Phelps A. S., Wilson P. W. 1941; Occurrence of hydrogen in nitrogen-fixing organisms. Proceedings of the Society of Experimental Biology and Medicine 47:473–476
    [Google Scholar]
  26. Russell E. W. 1973 Soil Conditions and Plant Growth. New York:: Longman.;
    [Google Scholar]
  27. Schubert K. R., Engelke J. A., Russell S. A., Evans H. J. 1977; Hydrogen reactions of nodulated leguminous plants. I. Effect of rhizobial strain and plant age. Plant Physiology 60:651–654
    [Google Scholar]
  28. Schubert K. R., Evans H. J. 1976; Hydrogen evolution: a major factor affecting the efficiency of nitrogen fixation in nodulated symbionts. Proceedings of the National Academy of Sciences of the United States of America 73:1207–1211
    [Google Scholar]
  29. Vincent J. M. 1974; Root-nodule symbiosis with Rhizobium. . In The Biology of Nitrogen Fixation pp. 265–341 Amsterdam:: North Holland.;
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-128-8-1829
Loading
/content/journal/micro/10.1099/00221287-128-8-1829
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error