1887

Abstract

A new class of regulatory mutants in the locus has been isolated by plating strains, under anaerobic conditions, on medium containing 1,2,4-triazole. The isolated mutants are cysteine prototrophs and triazole-resistant, although the levels of cysteine and -acetyl--serine sulphydrylase are not changed. In contrast to the constitutive mutants identified previously, the expression of the cysteine biosynthetic enzymes in the newly isolated mutants is regulated by the same factors as in wild-type strains. In the double mutant , the cysteine biosynthetic enzymes are absent with the exception of -acetyl--serine sulphydrylase.

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-128-8-1785
1982-08-01
2021-10-19
Loading full text...

Full text loading...

/deliver/fulltext/micro/128/8/mic-128-8-1785.html?itemId=/content/journal/micro/10.1099/00221287-128-8-1785&mimeType=html&fmt=ahah

References

  1. Cheney R. W., Kredich N. M. 1975; Fine-structure genetic map of the cysB locus in Salmonella typhimurium. . Journal of Bacteriology 124:1273–1281
    [Google Scholar]
  2. Filutowicz M., Hulanicka M. D. 1978; Delayed inducibility of bacterial sulphite reductase under anaerobic conditions. Abstracts of International Congress of Genetics, Moscow Cl: p 8
    [Google Scholar]
  3. Gornall A. G., Bardawill C. J., David M. M. 1949; Determination of serum protein by means of the biuret reaction. Journal of Biological Chemistry 177:751–766
    [Google Scholar]
  4. Hulanicka D., Klopotowski T. 1972; Mutants of Salmonella typhimurium resistant to triazole. Acta biochimica polonica 19:251–260
    [Google Scholar]
  5. Hulanicka M. D., Kredich N. M. 1976; A mutation affecting expression of the gene coding for serine transacetylase in Salmonella typhimurium. . Molecular and General Genetics 148:143–148
    [Google Scholar]
  6. Hulanicka M. D., Kredich N. M., Treiman D. M. 1974; The structural gene for O-acetylserine sulfhydrylase-A in Salmonella typhimurium. . Journal of Biological Chemistry 249:867–872
    [Google Scholar]
  7. Hulanicka M. D., Hallquist S. G., Kredich N. M., Mojica-A T. 1979; Regulation of O acetylserine sulfhydrylase-B by l-cysteine in Salmonella typhimurium. . Journal of Bacteriology 140:141–146
    [Google Scholar]
  8. Jagura G., Hulanicka D. 1978; Analysis of merodiploids of the cysB region in Salmonella typhimurium. . Molecular and General Genetics 165:31–38
    [Google Scholar]
  9. Jones-Mortimer M. C. 1968; Positive control of sulphate reduction in Escherichia coli. Isolation, characterization and mapping of cysteineless mutants of E. coli K12. Biochemical Journal 110:589–595
    [Google Scholar]
  10. Kredich N. M. 1971; Regulation of l-cysteine biosynthesis in Salmonella typhimurium. I. Effects of growth on varying sulfur sources and O-acetyl- l-serine on gene expression. Journal of Biological Chemistry 246:3474–3484
    [Google Scholar]
  11. Kredich N. M., Foote L. J., Hulanicka M. D. 1975; Studies on the mechanism of inhibition of Salmonella typhimurium by 1,2,4-triazole. Journal of Biological Chemistry 250:7324–7331
    [Google Scholar]
  12. Sakami W., Toennies G. 1942; The investigation of amino acid reactions by methods of non-aqueous titrimetry. Journal of Biological Chemistry 144:203–217
    [Google Scholar]
  13. Śledziewska E., Hulanicka D. 1978; Methods of isolation of cysteine constitutive mutants of the cysteine regulon in Salmonella typhimurium. . Molecular and General Genetics 165:289–293
    [Google Scholar]
  14. Smith H. O., Levine M. 1967; A phage P22 gene controlling integration of prophage. Virology 31:207–216
    [Google Scholar]
  15. Spencer H. T., Collins J., Monty K. J. 1967; Sequential regulation of cysteine biosynthesis in Salmonella typhimurium. . Federation Proceedings 26:677–679
    [Google Scholar]
  16. Tully M. D., Yudkin M. D. 1975; The nature of the product of the cysB gene of Escherichia coli. . Molecular and General Genetics 136:181–183
    [Google Scholar]
  17. Vito P. C., De, Dreyfuss J. 1964; Metabolic regulation of adenosinotriphosphate sulphydrylase in yeast. Journal of Bacteriology 88:1341–1348
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-128-8-1785
Loading
/content/journal/micro/10.1099/00221287-128-8-1785
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error